6가크롬 노출에 따 른 넙치, Paralichthys olivaceus의 혈액학적 성상 및 혈장성분의 변화
Effects of Exposure to Hexavalent Chromium on Hematological Parameters and Plasma Components in Flatfish, Paralichthys olivaceus
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본 연구는 넙치 치어 (평균 체장 13.3±1.6 cm, 평균 체중 25.6±3.7 g)를 수중 6가크롬에 10일간 0, 0.5,1.0, 2.0 mg L-1의 농도로 노출시켜 독성영향 판단하기 위해 수행되었다. 넙치 hemoglobin과 hematocrit와 같은 혈액학적 성상은 수중 크롬노출에 의해 유의적으로 감소하였다. 혈장 무기성분인 calcium과 magnesium은 크롬노출에 의해 유의적인 변화는 나타나지 않았다. 혈장 무기성분인 glucose와 cholesterol과 같은 1.0 mg L-1 이상의 크롬노출에 의해 유의적으로 증가하였지만, total protein은 유의적인 변화는 나타나지 않았다. 혈장 효소성분인 AST, ALT, ALP는 크롬노출에 의해 유의적인 증가가 나타났다. 본 실험의 결과는 수중 크롬노출은 넙치의 혈액 및 혈장성분에 유의적인 변화를 유발하며, 이러한 지표의 변화는 수중 크롬노출의 독성영향을 판단하는 주요한 지표가 될 것이다.
Abstract
Paralichthys olivaceus (mean length, 13.3±1.6 cm; mean weight, 25.6±3.7 g) were exposed to waterborne hexavalent chromium at different concentrations (0, 0.5, 1.0, and 2.0 mg L-1) for 10 days. Hematological parameters such as hemoglobin and hematocrit of P. olivaceus were significantly decreased after waterborne chromium exposure. There were no significant alterations in inorganic plasma components, calcium, or magnesium after waterborne chromium exposure. Organic plasma components such as glucose and cholesterol levels were significantly increased after exposure to chromium at concentration over 1.0 mg L-1. However, significant change in total protein was not observed. Enzymatic plasma components such as aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP) levels were significantly increased after chromium exposure. Results of this study indicate that waterborne chromium exposure can cause significant alterations in hematological parameters and plasma components of P. olivaceus. Such changes in parameters could be used as reliable indicators for toxic effects of waterborne chromium exposure.
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