양식 방사무늬김, Porphyra yezoensis의 활성처리제 처리 효과
Effects of a Commercial Activating Treatment Agent on Cultured Porphyra yezoensis thalli
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초록
활성처리제는 40∼200배의 희석액에 최단시간 1분, 최장시간 3분 침지 후의 방사무늬김과 파래의 사세포율은 방사무늬김은 유엽 및 성엽 모두 100∼150배 희석율이 사세포율이 낮게 나타났으며, 파래는 희석배율과 침지시간에 관계없이 90%이상 파래가 사멸하였다. 현장시험에서 방사무늬김 세포의 사세포율은 처리시기에 따라 차이가 나지만 처리 전은 2.1∼17.6% 였으며, 처리 후에는 2.1∼15.0%로 처리 전후의 사세포율에는 차이가 없었다. 파래의 사세포율은 처리 전에는 0∼4.6%였지만, 처리 후에는 99.0∼100%로 활성처리제의 처리는 파래 제거에 매우 효과적이었다. 또한 활성처리제 처리는 엽체의 부착 규조류는 33.3-97.2%, 부착 생균수는 1/10∼1/100,000로 감소시킨 반면 엽체의 색택에는 영향을 미치지 않았다. 그러므로 방사무늬김 엽체의 상태와 수온 등을 고려하여 본 활성처리제를 100∼150배의 희석농도에 1∼3분간 처리함으로써 파래의 제거, 부착 규조류의 탈락, 엽체 부착 생균수의 감소 효과가 있으며, 방사무늬김의 색택에는 별다른 영향을 미치지 않아 방사무늬김 활성처리제로서 유효한 것으로 나타났다.
Abstract
The use of activating treatment agent (formerly acid treatment agent) has been an effective strategy to remove deleterious epibiont organisms such as diatoms and green seaweeds, and it has greatly contributed to increase in Porphyra production. Although many manufacturers supply many kinds of activating treatment agent with different components in these days, no report about their effects on Porphyra culture was found. In this paper, effects of a commercial activating treatment agent were evaluated for practical use in Porphyra culture. No difference was found in dead cell ratios(%) of Porphyra yezoensis thalli between treated and control groups. However, dead cell ratios of Monostroma nitidum thalli were increased from 0∼4.6% to 99.0∼100% after the treatment. Bathing Porphyra thalli in activating treatment agent resulted in a great decrease in epiphytic bacterial number attached to the thalli from 102∼1011 cells/g to 0∼105 cells/g but did not change the colour of the thalli. These results suggest that bathing Porphyra thalli in activating treatment agent could be a promising strategy to remove green algae, diatoms and bacteria.
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