실내배양에서 방사무늬김의 황백화 회복에 대한 연구
A study on the recovery of discoloration of Pyropia yezoensis in laboratory culture
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초록
빈영양해수에서 배양하여 황백화를 유도한 김 엽체는 NO3- (300 μM)와 PO4 3- (30 μM)를 공급하여 시간의 경과에 따른 회복 정도를 분석하였다. NO3- 공급 조건에서는 배양 2일 후부터 김 엽체의 회복이 나타났으며, PO4 3- 공급 조건에서는 배양 5일 후부터 김 엽체의 회복이 관찰되었다. 색차계의 수치화 된 값들은 황백화 김 엽체의 회복을 판단하는데 유용하였다. 황백화 김은 무기영양분의 공급으로 비대한 액포가 축소되고, 색택이 회복되는 것으로 나타났다.
Abstract
To recover the discoloration of Pyropia yezoensis, additional NO3- and PO4 3- nutrients were supplied to the discolored blades. As a result, the efficiency of photosynthesis was increased from two days after incubation with NO3- and five days after incubation with PO4 3-. In addition, the chromaticity analysis results showed significant changes. Furthermore, the cell vacuoles bloated due to discoloration were reduced again when they were restored from discoloration, suggesting that the control of discoloration is feasible by nutrient supply.
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