어류병원성 Edwardsiella spp.의 2010년 이전 분리균의 재분류와 특성
Reclassification of fish pathogenic Edwardsiella spp. isolated before 2010 and their characteristics
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Edwardsiella piscicida previously identified as E. tarda is an important pathogen of numerous fish species. In this study, reclassification was conducted on 17 strains isolated from diseased cultured olive flounder or eel and identified as E. tarda between 1996 and 2010 in Korea. For this, the detection of a polymerase chain reaction (PCR) amplicon by species-specific primers and phylogenetic analysis based on gyrB sequences were performed. And, nucleotide sequences of three amplicons detected in RAPD profile of the isolates originated from eel and the antimicrobial susceptibility were compared. Only 17 isolates formed an amplicon in PCR using E. piscicida primer. The gyrB of the 17 isolates diverged with those of the E. piscicida reference strains in a phylogenetic tree constructed with MEGA11 software. Regions matching the nucleotide sequences of the RAPD amplicons were identified in Edwardsiella phage GF-2, E. piscicida plasmid pEPMS-18199 and E. anguilllarum ET080813 plasmid 1. The antimicrobial susceptibility of the isolates originated from flounder showed non-wild type (NWT) for doxycycline, oxytetracycline, and tetracycline, and only isolates derived from eel were NWT for oxolinic acid and flumequine. These results confirmed that all 17 isolates of this study classified as E. piscicida, and that the characteristics of the isolates showed differences depending on the isolate’s host.
Abstract
Edwardsiella piscicida previously identified as E. tarda is an important pathogen of numerous fish species. In this study, reclassification was conducted on 17 strains isolated from diseased cultured olive flounder or eel and identified as E. tarda between 1996 and 2010 in Korea. For this, the detection of a polymerase chain reaction (PCR) amplicon by species-specific primers and phylogenetic analysis based on gyrB sequences were performed. And, nucleotide sequences of three amplicons detected in RAPD profile of the isolates originated from eel and the antimicrobial susceptibility were compared. Only 17 isolates formed an amplicon in PCR using E. piscicida primer. The gyrB of the 17 isolates diverged with those of the E. piscicida reference strains in a phylogenetic tree constructed with MEGA11 software. Regions matching the nucleotide sequences of the RAPD amplicons were identified in Edwardsiella phage GF-2, E. piscicida plasmid pEPMS-18199 and E. anguilllarum ET080813 plasmid 1. The antimicrobial susceptibility of the isolates originated from flounder showed non-wild type (NWT) for doxycycline, oxytetracycline, and tetracycline, and only isolates derived from eel were NWT for oxolinic acid and flumequine. These results confirmed that all 17 isolates of this study classified as E. piscicida, and that the characteristics of the isolates showed differences depending on the isolate’s host.
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