동물행동모델에서 구기자 추출물의 피로회복 효과에 대한 연구
Anti-fatigue effect of Lycii Fructus in a behavioral mouse model
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본 연구의 목적은 강제부하수영시험을 이용하여 구기자의 피로회복 효과를 알아보는 것으로 실험은 수컷 ICR 마우스를 무작위로 식염수 처리-수영 부하 테스트 그룹(대조군) 및 구기자 추출물처리(1, 10, 100 mg/kg)-수영 부하 테스트 그룹(실험군)으로 나누어 진행하였다. 강제 수영으로 피로를 유발하고 마우스의 부동 시간, 산소소모량, 생화학적 요인들을 분석하였다. 마우스에게 생리식염수 또는 구기자 추출물을 실험 전에 2주 동안 매일 1회 경구 투여하였다. 강제부하수영 후 부동시간 및 산소 소모량을 측정하였고, 생화학적 분석을 위해 혈청을 채취하였다 . 실험군은 부동시간, 산소 소모량, 젖산, 젖산탈수소효소가 대조군에 비해 감소하였다. 강제부하수영 후 실험군에서대조군에 비해 에너지원(중성지방 및 유리지방산) 소비 및 에너지 회수가 향상되었다. 이러한 결과는 구기자 추출물이 피로 회복을 위한 잠재적인 영양식품일 수 있음을 시사한다.
Abstract
Lycii fructus (LF, Lycium chinese Miller) is an economically and agriculturally significant perennial fruit tree crop of the Solanaceae family and is used as a traditional herbal medicine. The aim of this study was to examine anti-fatigue effect of LF using the forced swimming test (FST). Male ICR mice were randomly divided into two groups; Saline treatment-swimming load test group (control) and LF supplied swimming group. Fatigue was induced by forced swimming and the immobility time was analyzed in mice. The mice were orally administered before FST one daily for 2 weeks. After the FST, oxygen consumption was measured by physiological test, and serum was collected for biochemical analysis. Immobility, oxygen consumption and biochemical factors were increased that of control group. LF group was significantly decreased immobility time compared to control group. In addition, oxygen consumption was also significantly decreased by LF treatment. The increase of lactic acid and lactate dehydrogenase after FST was inhibited by LF treatment. Consumption of energy sources (free fatty acid and triglyceride) and energy recovery were improved by LF treatment after FST. All the results suggest LF might be a potentially health food for a recovery form fatigue
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