A report of 9 unrecorded radiation resistant bacterial species in Korea
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Five bacterial strains, ES10-3-3-1, KKM10-2-2-1, Ant11, JM10-4-1-3, and KMS4-11 assigned to the genus Deinococcus were isolated from soil samples collected from Namyangju-si in Gyeonggi-do, Gangnam-gu and Dongdaemun-gu in Seoul, Korea. In addition, four bacterial strains, KKM10-2-7-2, JM10-2-5, JM10-2-6-2, and KKM10-2-3 assigned to the genus Hymenobacter were isolated from soil samples collected from Gangnam-gu and Dongdaemun-gu in Seoul, in South Korea. The five Deinococcus species were Gram-stain positive, pink-pigmented, and short-rod or coccus shaped. The four Hymenobacter species were Gram-stain negative, red-pigmented, and short-rod shaped. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ES10-3-3-1, KKM10-2-2-1, Ant11, JM10-4-1-3, and KMS4-11 were most closely related to Deinococcus citri NCCP-154T (with 99.8% similarity), Deinococcus grandis DSM 12784T (99.0%), Deinococcus marmoris DSM 12784T (98.8%), Deinococcus claudionis PO-04-19-125T (98.7%), and Deinococcus radioresistens 8AT (99.8%), respectively. KKM10-2-7-2, JM10-2-5, JM10-2-6-2, and KKM10-2-3 were most closely related to Hymenobacter algoricola VUG-A23aT (99.1% similarity), Hymenobacter elongatus VUG-A112T (99.1% similarity), Hymenobacter gelipurpurascens Txg1T (99.1% similarity), and Hymenobacter psychrotolerans Tibet-IIU11T (99.3% similarity), respectively. These nine species have never been reported in Korea; thus, five Deinococcus species are reported in the family Deinococcaceae, order Deinococcales, class Deinococci, phylum Deinococcus-Thermus and four Hymenobacter species are reported in the family Cytophagaceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes.
Abstract
Five bacterial strains, ES10-3-3-1, KKM10-2-2-1, Ant11, JM10-4-1-3, and KMS4-11 assigned to the genus Deinococcus were isolated from soil samples collected from Namyangju-si in Gyeonggi-do, Gangnam-gu and Dongdaemun-gu in Seoul, Korea. In addition, four bacterial strains, KKM10-2-7-2, JM10-2-5, JM10-2-6-2, and KKM10-2-3 assigned to the genus Hymenobacter were isolated from soil samples collected from Gangnam-gu and Dongdaemun-gu in Seoul, in South Korea. The five Deinococcus species were Gram-stain positive, pink-pigmented, and short-rod or coccus shaped. The four Hymenobacter species were Gram-stain negative, red-pigmented, and short-rod shaped. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ES10-3-3-1, KKM10-2-2-1, Ant11, JM10-4-1-3, and KMS4-11 were most closely related to Deinococcus citri NCCP-154T (with 99.8% similarity), Deinococcus grandis DSM 12784T (99.0%), Deinococcus marmoris DSM 12784T (98.8%), Deinococcus claudionis PO-04-19-125T (98.7%), and Deinococcus radioresistens 8AT (99.8%), respectively. KKM10-2-7-2, JM10-2-5, JM10-2-6-2, and KKM10-2-3 were most closely related to Hymenobacter algoricola VUG-A23aT (99.1% similarity), Hymenobacter elongatus VUG-A112T (99.1% similarity), Hymenobacter gelipurpurascens Txg1T (99.1% similarity), and Hymenobacter psychrotolerans Tibet-IIU11T (99.3% similarity), respectively. These nine species have never been reported in Korea; thus, five Deinococcus species are reported in the family Deinococcaceae, order Deinococcales, class Deinococci, phylum Deinococcus-Thermus and four Hymenobacter species are reported in the family Cytophagaceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes.
참고문헌 (24)
- Ahmed, I., "Deinococcus citri sp. nov., isolated from citrus leaf canker lesions", Int J Syst Evol Microbiol, 64, pp.4134~4140 (2014)
- Brooks, B.W., "Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species", Int J Syst Bacteriol, 19, pp.353~360 (1981)
- Buczolits, S., "Proposal of Hymenobacter norwichensis sp. nov., classification of ‘Taxeobacter ocellatus’, ‘Taxeobacter gelupurpurascens’ and ‘Taxeobacter chitinovorans’ as Hymenobacter ocellatus sp. nov., Hymenobacter gelipurpurascens sp. nov. and Hymenobacter chitinivorans sp. nov., respectively, and emended description of the genus Hymenobacter Hirsch", Int J Syst Evol Microbiol, 56, pp.2071~2078 (2006)
- Callegan, R. P., "Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments", Int J Syst Evol Microbiol, 58, pp.1252~1258 (2008)
- Doetsch, R.N., "Determinative methods of light microscopy. Manual of Methods for General Bacteriology", pp.21~33 (1981), American Society for Microbiology
- Edgar, R.C., "MUSCLE: multiple sequence alignment with high accuracy and high throughput", Nucleic Acids Res, 32, pp.1792~1797 (2004)
- Felsenstein, J., "Confidence limit on phylogenies : an approach using the bootstrap", Evolution, 39, pp.783~791 (1985)
- Hall, T.A., "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95", 98, Nucleic Acids Symp Ser, pp.1999, 41[DOI]
- Han, L., "Hymenobacter qilianensis sp. nov., isolated from a subsurface sandstone sediment in the permafrost region of Qilian Mountains, China and emended description of the genus Hymenobacter", Antonie van Leeuwenhoek, 105, pp.971~978 (2014)
- Hirsch P., "Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica", Syst Appl Microbiol, 27, pp.636~645 (2004)
- Hirsch, P., "Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctic soils and sandstone: bacteria of the Cytophaga", Flavobacterium, Syst Appl Microbiol, pp.1998, 21[DOI]
- Kampfer, P., "Deinococcus aquatilis sp. nov., isolated from water", Int J Syst Evol Microbiol, 58, pp.2803~2806 (2008)
- Kim, O. S., "Introducing EzTaxon-e : a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species", Int J Syst Evol Microbiol, 62, pp.716~721 (2012)
- Klassen, J.L., "Characterization of Hymenobacter isolates from Victoria Upper Glacier, Antarctica reveals five new species and substantial nonvertical evolution within this genus", Extremophiles, 15, pp.45~57 (2011)
- Oyaizu, H., "A radiation-resistant rod-shaped bacterium, Deinobacter grandis gen. nov., sp. nov., with peptidoglycan containing ornithine", Int J Syst Bacteriol, 37, pp.62~67 (1987)
- Rainey, F. A., "Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison", Int J Syst Bacteriol, 47, pp.510~514 (1997)
- Saitou, N., "The neighbor-joining method : a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Srinivasan, S., "Deinococcus radioresistens sp. nov., a UV and gamma radiation-resistant bacterium isolated from mountain soil", Antonie Van Leeuwenhoek, 107, pp.539~545 (2015)
- Srinivasan, S., "Deinococcus daejeonensis sp. nov., isolated from sludge in a sewage disposal plant", Int J Syst Evol Microbiol, 62, pp.1265~1270 (2012)
- Srinivasan, S., "Deinococcus humi sp. nov., isolated from soil", Int J Syst Evol Microbiol, 62, pp.2844~2850 (2012)
- Tamura, K., "Mega5 : Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol Biol Evol, 28, pp.2731~2739 (2011)
- Weisburg, W. G., "16S ribosomal DNA amplification for phylogenetic study", J Bacteriol, 173, pp.697~703 (1991)
- Wragg, P., "Comparison of Biolog GEN III MicroStation semi-automated bacterial identification system with matrix-assisted laser desorption ionization-time of flightmass spectrometry and 16S ribosomal RNA gene sequencing for the identification of bacteria of veterinary interest", J Microbiol Methods, 105, pp.16~21 (2014)
- Zhang, G., "Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai-Tibet Plateau permafrost region", Int J Syst Evol Microbiol, 58, pp.1215~1220 (2008)