순종 랜드레이스의 10주령 체중 형질에 영향을 미치는 후보유전자 탐색을 위한 전장유전체 분석
Genome-wide Association Study Analysis for Explore of Candidate Genes Affecting Weight of 10 Week Old Traits in Purebred Landrace
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초록
가축검정기준(Livestock Inspection Standards)에 따르면 약 30 kg에 입식을 하여 예비사육기간 이후 검정을 시작한다. 이는 10주령 시기까지 체내 기관의 온전한 성장과 질병에 문제가 없는 검정자돈을 선발하기 위함이며, 또한 10주령 시기는 근섬유의 크기가 최대치로 증가하고 이후 비육시기의 지방 침착을 위해 준비하는 시기이다. 이에 본 논문은 순종 랜드레이스 집단 703두와 genome-wide complex trait analysis(GCTA) 프로그램의 mlma command를 통한 전장유전체 연관성 분석(genome-wide association study, GWAS)을 수행하여 10주령 체중과 연관된 후보유전자를 탐색하였다. 전장유전체 연관성 분석 결과 genome-wide suggestive level의 유의성을 가지는 single nucleotide polymorphism(SNP) marker가 6번 염색체에서 3개 동정되었다(DIAS0002615; p value=1.62×10-6, MARC 0083933; p value=4.94×10-6, ASGA0028717; p value=5.40× 10-6). 이 3개의 SNP marker가 위치한 2개의 유전자(Ubiquitin protein ligase E3 component n-recognin 4; UBR4, WD and tetratricopeptide repeats 1; WDTC1)들은 순종 랜드레이스 집단의 10주령 체중의 위치상 후보 유전자이며, 두 개의 후보유전자는 비육과 연관된 유전자로 보고되어 있다. 따라서 본 연구의 위치상 후보유전자인 UBR4, WDTC1은 타 집단과 추가적인 실험 연구를 통해 10주령 체중과 비육 연관 형질의 유전자로서 이용이 가능할 것으로 생각된다.
Abstract
According to Livestock Inspection Standards, the piglets enter the feedlot at approximately 30 kg, and the inspection starts after the preliminary feeding period. The reason for applying the preliminary feeding period is to select inspection piglets with no diseases after the complete growth of the internal organs until 10 weeks of age. Furthermore, the age of 10 week is the time when the muscle fibers grow to their maximum size and the piglets are prepared for fat deposition at the later fattening period. In the study, a genome-wide association study (GWAS) was performed through the mlma command of the genome-wide complex trait analysis (GCTA) program with 703 purebred Landrace population, and the candidate genes associated with the weight of 10 week were searched. The GWAS identified 3 single nucleotide polymorphism (SNP) markers, which have a significant genome-wide suggestive level, on chromosome 6 (DIAS0002615; p value=1.62×10-6, MARC0083933; p value=4.94×10-6, ASGA0028717; p value=5.40×10-6). The 2 genes (Ubiquitin protein ligase E3 component n-recognin 4; UBR4, WD and tetratricopeptide repeats 1; WDTC1) in which these 3 SNP markers are located are positional candidate genes of the weight of 10 week of the purebred Landrace population. 2 candidate genes have been reported to be associated with fattening. Therefore, the positional candidate genes in this study, UBR4 and WDTC1, are expected to be usable as genes for traits associated with the weight of 10 week weight and fattening through additional experimental research with other population
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