돼지 자궁내막세포에서 Plasminogen Activator mRNA와 단백질 발현에 미치는 Heat Stress의 영향
Effects of Heat Stress on Expression of Plasminogen Activators mRNA and Protein in Porcine Endometrial Cells
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The aim of this study was to investigate change of plasminogen activators (PAs) and their inhibitors (PAIs) mRNA and protein expression level by heat stress in porcine endometrial cells. The endometrial epithelial cells were isolated from endometrial epithelium in porcine uterus and cultured in different temperature conditions (38.5 and 41.5℃) for 24 h. Expression of urokinase-type PA (uPA), tissue-type PA (tPA), PA inhibitor-1 (PAI-1) and -2 (PAI-2) mRNA in epithelial cells were analyzed using reverse transcription-PCR and protein levels were measured by immunofluorescence. In result, mRNA expression of uPA, tPA, PAI-1 and PAI-2 were decreased in 41.5℃ than 38.5℃ culture condition, however, significant differences were no detected. uPA, tPA and PAI-2 protein were mainly expressed in nucleus, whereas PAI-1 was distributed in cytoplasm and nucleus. uPA and tPA protein levels were increased by heat stress treatment and significant difference was only detected in tPA level (p<0.05). In contrast, two types of PAIs protein level were decreased in 41.5℃ cultured group compared with 38.5℃ group. In present study, tPA protein expression was upregulated by heat stress in porcine endometrial cells. This result suggest that change of tPA by heat stress may be related to blood flow into uterus and intrauterine microenvironments, and could directly and indirectly influence to reproductive performance in pigs.
Abstract
The aim of this study was to investigate change of plasminogen activators (PAs) and their inhibitors (PAIs) mRNA and protein expression level by heat stress in porcine endometrial cells. The endometrial epithelial cells were isolated from endometrial epithelium in porcine uterus and cultured in different temperature conditions (38.5 and 41.5℃) for 24 h. Expression of urokinase-type PA (uPA), tissue-type PA (tPA), PA inhibitor-1 (PAI-1) and -2 (PAI-2) mRNA in epithelial cells were analyzed using reverse transcription-PCR and protein levels were measured by immunofluorescence. In result, mRNA expression of uPA, tPA, PAI-1 and PAI-2 were decreased in 41.5℃ than 38.5℃ culture condition, however, significant differences were no detected. uPA, tPA and PAI-2 protein were mainly expressed in nucleus, whereas PAI-1 was distributed in cytoplasm and nucleus. uPA and tPA protein levels were increased by heat stress treatment and significant difference was only detected in tPA level (p<0.05). In contrast, two types of PAIs protein level were decreased in 41.5℃ cultured group compared with 38.5℃ group. In present study, tPA protein expression was upregulated by heat stress in porcine endometrial cells. This result suggest that change of tPA by heat stress may be related to blood flow into uterus and intrauterine microenvironments, and could directly and indirectly influence to reproductive performance in pigs.
참고문헌 (38)
- Aflalo, E., "Expression of plasminogen activators in preimplantation rat embryos developed in vivo and in vitro", Reprod. Biol. Endocrinol, 3, pp.7~ (2005)
- Andersson, H., "Photoperiodic effects on pubertal maturation of spermatogenesis, pituitary responsiveness to exogenous GnRH, and expression of boar taint in crossbred boars", Anim. Reprod. Sci, 54, pp.121~137 (1998)
- Asuthkar, S., "Multifunctional roles of urokinase plasminogen activator (uPA) in cancer stemness and chemoresistance of pancreatic cancer", Mol. Biol. Cell, 24, pp.2620~2632 (2013)
- Bazer, F. W., "Pregnancy recognition signaling mechanisms in ruminants and pigs", J. Anim. Sci. Biotechno, 4, pp.23~ (2013)
- Baumans, W., "Recommendations for euthanasia of experimental animals: Part 2", Laboratory Animals, 31, pp.1~32 (1997)
- Beers, W. H, "Follicular plasminogen and plasminogen activator and the effect of plasmin on ovarian follicle wall", Cell, 6, pp.379~386 (1975)
- Carmeliet, P., "Gene manipulation and transfer of the plasminogen and coagulation system in mice", Semin. Thromb. Hemost, 22, pp.525~542 (1996)
- Claus, R., "Influence of light and photoperiodicity on pig prolificacy", J. Reprod. Fertil. Supplement, 33, pp.199~208 (1985)
- Dourmad, J. Y., "The effect of energy and protein intake of sows on their longevity: a review", Livest. Prod. Sci, 40, pp.87~97 (1994)
- Estreicher, A., "Characterization of the celluar binding site for the urokinase-type plasminogen activator", J. Biol. Chem, 264, pp.1180~1189 (1989)
- Fazleabas, A. T., "Relationship between release of plasminogen activator and estrogen by blastocysts and secretion of plasmin inhibitor by uterine endometrium in pregnant pig", Bio. Reprod, 29, pp.222~238 (1983)
- Finlay, T. H., "Estrogen-stimulated uptake of plasminogen by the mouse uterus", Endocrinology, 112, pp.856~861 (1983)
- Hu, Z. Y., "Expression of tissuetype and urokinase-type plasminogen activators as well as plasminogen activator inhibitor type-1 and type-2 in human and rhesus monkey placenta", J. Anat, 194, pp.183~195 (1999)
- Huarte, J., "Involvement of the plasminogen activator", plasmin proteolytic cascade in fertilization, 1993, pp.157, 539~546
- Jeong, W., "Lysophosphatidic acid (LPA) receptor 3-mediated LPA signal transduction pathway: A possible relationship with early development of peri-implantation porcine conceptus", Biol. Reprod, 115, pp.137~174 (2016)
- Kobayashi, T., "Trypsin-like arginine amidases including plasminogen and plasmin in human seminal plasma by affinity adsorption and elution", Arch. Androl, 28, pp.165~170 (1992)
- Kobayashi, Y., "Summer heat stress affects prostaglandin synthesis in the bovine oviduct", Reproduction, 146, pp.103~110 (2013)
- Krania, F., "Effects of addition of tissue-type plasminogen activator in in vitro fertilization medium on bovine embryo development and quality", Reprod. Dom. Anim, 50, pp.112~120 (2015)
- Li, Y., "Melatonin protects porcine oocyte in vitro maturation from heat stress", J. Pineal Res, 59, pp.365~375 (2015)
- Liu, K., "Coordinated expression of tissuetype plasminogen activator and plasminogen activator inhibitor type-1 during corpus luteum formation and luteolysis in the adult pseudopregnant rat", Endocrinology, 137, pp.2126~2132 (1996)
- Liu, Y. X., "Localisation and distribution of tissue-type and urokinase-type plasminogen activators and their inhibitors type-1 and type-2 in human and rhesus monkey fetal membranes", J. Anat, 194, pp.183~195 (1998)
- Liu, Y. X., "Tissue-specific and time-coordinated hormone regulation of plasminogen-activator-inhibitor type-1 and tissue-type plasminogen activator during gonadotrophin induced ovulation", Eur. J. Biochem, 195, pp.549~555 (1991)
- Love, R. J., "Seasonal effects on fertility in gilts and sows", J. Reprod. Fertil. Supplement, 48, pp.191~206 (1993)
- Love, R. J., "An interaction between feeding rate and season affects fertility of sows", Anim. Reprod. Sci, 39, pp.275~284 (1995)
- Malayer, J., "Regulation of heat shock-induced alterations in the release of prostaglandins by the uterine endometrium of cows", Theriogenology, 34, pp.219~230 (1990)
- Mekkawy, A. H., "Involvement of urokinase-type plasminogen activator system in cancer: an overview", Med. Res. Rev, 34(5), pp.918~956 (2014)
- Mignatti, P., "Biology and biochemistry of proteinase in tumor invasion", Physiol. Rev, 73, pp.161~195 (1993)
- Myohanen, H, "Regulation and interactions in the activation of cell-associated plasminogen", Cell. Mol. Life. Sci, 61, pp.2840~2858 (2004)
- Peltoniemi, O. A. T., "Seasonal and management effects on fertility of the sow: a descriptive study", Anim. Reprod. Sci, 55, pp.47~61 (1999)
- Perltoniemi, O. A. T., "Factors effecting reproduction in the pig: seasonal effects and restricted feeding of the pregnant gilt and sow", Anim. Reprod. Sci, 60, pp.173~184 (2000)
- Prunier, A., "Influence of high temperature on performance of reproductive sows", Livest. Prod. Sci, 52, pp.123~133 (1997)
- Prunier, A., "Environmental and seasonal influences on the return-to-oestrus after weaning in primiparous sows: a review", Livest. Prod. Sci, 45, pp.103~110 (1996)
- Rensis, F. D., "Heat stress and seasonal effects on reproduction in the dairy cow-a review", Theriogenology, 60, pp.1139~1151 (2003)
- Roth, Z., "Delayed effect of heat stress on steroid production in medium-sized and preovulatory bovine follicles", Reproduction, 121, pp.745~751 (2001)
- Sartori, R., "Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in winter", J. Dairy Sci, 85, pp.2803~2812 (2002)
- Schoenherr, W. D., "The effects of dietary fat or fiber addition on yield and composition of milk from sows housed in a warm or hot environment", J. Anim. Sci, 67, pp.482~495 (1989)
- Teesalu, T., "Embryo implantation in mouse: fetomaternal coordination in the pattern of expression of uPA, uPAR, PAI-1 and _2MR", LRP genes, 1996, pp.56, 103~116
- Zhang, Y., "Urokinase plasminogen activator system targeted delivery of nanobins as a novel ovarian cancer therapeutics", Mol. Cancer Ther, 12(12), pp.2628~2639 (2013)