Effects of 17β-estradiol, Interleukin-1β, and Human Chorionic Gonadotropin on Activity and mRNA Expression of Plasminogen Activators in Porcine Endometrial Cells
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This study aimed to investigate changes in the activity and mRNA expression of plasminogen activators (PAs) induced by 17β-estradiol (E2), human chorionic gonadotropin (hCG), and interleukin-1β (IL-1β) in porcine endometrial cells. Endometrial cells were isolated from the epithelium and cultured to 80% confluence. They were then treated for 24 h with E2 (0.2, 2, 20, and 200 ng/mL), IL-1β (0.1, 1, 10, and 100 ng/mL), and hCG (0.5, 1, 1.5 and 2 IU/mL). mRNA expressions of urokinase-type (uPA) and tissue-type (tPA) PAs were analyzed using reverse transcription PCR, and activities were measured using a PA activity assay. mRNA expressions of uPA and tPA increased with E2 treatment; however, this was not significant. Similarly, treatment with hCG did not influence the mRNA expressions of PAs. Interestingly, treatment with 0.1 ng/mL IL-1β significantly reduced the mRNA expression of uPA, but did not affect that of tPA. Treatment with 2, 20, and 200 ng/mL E2 increased PA activity compared with the control group; treatment with 0.1 and 1 ng/mL IL-1β significantly increased PA activity compared with the other IL-1β treatment groups, whereas treatment with 10 and 100 ng/mL IL-1β decreased. Treatment with 2 IU/mL hCG increased PA activity compared with the other treatment groups, although there were no significant differences between the hCG and control groups. In conclusion, the activity and mRNA expression of PAs were differently regulated by the hormone/cytokine and its concentration in porcine endometrial cells. Therefore, understanding PA regulatory mechanisms may help to improve the reproductive potential of domestic animals.
Abstract
This study aimed to investigate changes in the activity and mRNA expression of plasminogen activators (PAs) induced by 17β-estradiol (E2), human chorionic gonadotropin (hCG), and interleukin-1β (IL-1β) in porcine endometrial cells. Endometrial cells were isolated from the epithelium and cultured to 80% confluence. They were then treated for 24 h with E2 (0.2, 2, 20, and 200 ng/mL), IL-1β (0.1, 1, 10, and 100 ng/mL), and hCG (0.5, 1, 1.5 and 2 IU/mL). mRNA expressions of urokinase-type (uPA) and tissue-type (tPA) PAs were analyzed using reverse transcription PCR, and activities were measured using a PA activity assay. mRNA expressions of uPA and tPA increased with E2 treatment; however, this was not significant. Similarly, treatment with hCG did not influence the mRNA expressions of PAs. Interestingly, treatment with 0.1 ng/mL IL-1β significantly reduced the mRNA expression of uPA, but did not affect that of tPA. Treatment with 2, 20, and 200 ng/mL E2 increased PA activity compared with the control group; treatment with 0.1 and 1 ng/mL IL-1β significantly increased PA activity compared with the other IL-1β treatment groups, whereas treatment with 10 and 100 ng/mL IL-1β decreased. Treatment with 2 IU/mL hCG increased PA activity compared with the other treatment groups, although there were no significant differences between the hCG and control groups. In conclusion, the activity and mRNA expression of PAs were differently regulated by the hormone/cytokine and its concentration in porcine endometrial cells. Therefore, understanding PA regulatory mechanisms may help to improve the reproductive potential of domestic animals.
참고문헌 (36)
- Shin-Hye Ahn, "Relationship between Plasminogen Activity and Plasminogen Inhibitor during the Culture of Porcine Oviduct Epithelial Cells", Reproductive & Developmental biology, 33(4), pp.203~209 (2009), 사단법인 한국동물생명공학회
- Baker VL, "Reproductive endocrine and endometrial effects of raloxifene hydrochloride, a selective estrogen receptor modulator, in women with regular menstrual cycles 1", J Clin Endocrinol Metab, 83, pp.6~13 (1998)
- Bazer FW, "Pig blastocyst-uterine interactions", Differentiation, 87, pp.52~65 (2014)
- Beers WH, "Follicular plasminogen and plasminogen activator and the effect of plasmin on ovarian follicle wall", Cell, 6, pp.379~386 (1975)
- Bolzan E, "The novel effect of hCG administration on luteal function maintenance during the estrous cycle", pregnancy and early embryo development in the pig, 2013, pp.16, 323~332
- Brussow KP, "Studies on fixed-time ovulation induction in the pig", Soc Reprod Fertil Suppl, 66, pp.187~195 (2009)
- 전용필, "착상전 생쥐 자궁에서 콜라겐의 변화", 발생과 생식, 11(1), pp.1~11 (2007), 한국발생생물학회
- 전용필, "Androgen in the Uterus: A Compensator of Estrogen and Progesterone", 발생과 생식, 13(3), pp.133~143 (2009), 한국발생생물학회
- Coy P, "Oocytes use the plasminogen- plasmin system to remove supernumerary spermatozoa", Hum Reprod, 27, pp.1985~1993 (2012)
- Demir R, "Vasculogenesis and angiogenesis in the endometrium during menstrual cycle and implantation", Acta Histochem, 112, pp.203~214 (2010)
- Ebisch IMW, "Review of the role of the plasminogen activators system and vascular endothelial growth factor in subfertility", Fertil Steril, 90, pp.2340~2350 (2008)
- Finlay TH, "Estrogen-stimulated uptake of plasminogen by the mouse uterus", Endocrinology, 112, pp.856~861 (1983)
- Franczak A, "Transcriptomic analysis of the porcine endometrium during early pregnancy and the estrous cycle", Reprod Biol, 13, pp.229~237 (2013)
- Giordano JO, "Effect of presynchronization with human chorionic gonadotropin or gonadotropin-releasing hormone 7 days before resynchronization of ovulation on fertility in lactating dairy cows", J Dairy Sci, 95, pp.5612~5625 (2012)
- 황보용, "돼지의 배란 전 자궁내막 상피세포 내 Plasminogen Activators의 발현", 한국동물생명공학회지, 28(3), pp.257~263 (2013), 사단법인 한국동물생명공학회
- Jeong W, "Stimulatory effects of interleukin-1 beta on development of porcine uterine epithelial cell are mediated by activation of the ERK1", 2 MAPK cell signaling cascade, 2016, pp.419, 225~234
- Kang-Hyun Kim, "Changes in Plasminogen Activity in Uterus Tissue during the Estrous Cycle in the Pigs", Reproductive & Developmental biology, 35(4), pp.463~468 (2011), 사단법인 한국동물생명공학회
- Kobayashi T, "Trypsin-like arginine amidases including plasminogen and plasmin in human seminal plasma by affinity adsorption and elution", Arch Androl, 28, pp.165~170 (1992)
- Kouba AJ, "Oviductal plasminogen activator inhibitor (PAI-1): mRNA, protein, and hormonal regulation during the estrous cycle and early pregnancy in the pig", Mol Reprod Dev, 56, pp.378~386 (2000)
- Krania F, "Effects of addition of tissuetype plasminogen activator in in vitro fertilization medium on bovine embryo development and quality", Reprod Domest Anim, 50, pp.112~120 (2015)
- Lai MD, "Expression of proliferating cell nuclear antigen in luminal epithelium during the growth and regression of rat uterus", J Endocrinol, 166, pp.87~93 (2000)
- Liu YX, "Gonadotropin regulation of tissue-type and urokinase- type plasminogen activators in rat granulosa and theca-interstitial cells during the periovulatory period", Mol Cell Endocrinol, 54, pp.221~229 (1987)
- Malysz-Cymborska I, "Effect of hCG and eCG treatments on prostaglandins synthesis in the porcine oviduct", Reprod Domest Anim, 48, pp.1034~1042 (2013)
- Martin O, "Plasminogen activator production by trophoblast cells in vitro: Effect of steroid hormones and protein synthesis inhibitors", Am J Obstet Gynecol, 142, pp.402~409 (1982)
- Menshikov M, "Urokinase plasminogen activator stimulates vascular smooth muscle cell proliferation via redox-dependent pathways", Arterioscler Thromb Vasc Biol, 26, pp.801~807 (2006)
- Ny T, "Cultured granulosa cells produce two plasminogen activators and an ant iactivator, each regulated differently by gonadotropins", Endocrinology, 166, pp.1666~1668 (1985)
- Olofsson B, "Vascular endothelial growth factor B (VEGF-B) binds to VEGF receptor-1 and regulates plasminogen activator activity in endothelial cells", Proc Natl Acad Sci USA, 95, pp.11709~11714 (1998)
- Ploplis VA, "Plasminogen deficiency differentially affects recruitment of inflammatory cell populations in mice", Blood, 91, pp.2005~2009 (1998)
- Ross JW, "Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization", Reprod Biol Endocrinol, 1, pp.23~ (2003)
- 사수진, "Effects of Reactive Oxygen Species (ROS) on Sperm Function and Plasminogen Activator Activity in Porcine Spermatozoa", Reproductive & Developmental biology, 34(3), pp.185~191 (2010), 사단법인 한국동물생명공학회
- Shemesh M, "Expression of functional luteinizing hormone (LH) receptor and its messenger ribonucleic acid in bovine endometrium: LH augmentation of cAMP and inositol phosphate in vitro and human chorionic gonadotropin (hCG) augmentation of peripheral prostaglandin in vivo", Reprod Biol, 1, pp.13~32 (2001)
- Stormshak F, "Board-invited review: Estrogen and progesterone signaling: Genomic and nongenomic actions in domestic ruminants", J Anim Sci, 86, pp.299~315 (2008)
- Stroband HWJ, "The ultrastructure of the uterine epithelium of the pig during the estrous cycle and early pregnancy", Cell Tissue Res, 246, pp.81~89 (1986)
- Subramaniam S, "The interleukin 1 receptor family", Dev Comp Immunol, 28, pp.415~428 (2004)
- Norihiro Sugino, "Molecular mechanisms of luteinization", Obstetrics & Gynecology Science, 57(02), pp.93~101 (2014), 대한산부인과학회[DOI]
- Wongkaweewit K, "Effect of the administration of GnRH or hCG on time of ovulation and the onset of estrus-toovulation interval in sows in Thailand", Trop Anim Health Pro, 44, pp.467~470 (2012)