맥문동(Liriope platyphylla)과 유기산 첨가 시 호흡챔버 시스템을 이용한 염소의 장내 발효 메탄가스 저감 효과 평가
Effect of Liriope platyphylla and Organic Acids on Enteric Methane Mitigation of Goats Using Respiration Chamber System
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초록
본 연구는 메탄 발생량을 측정하는 간이호흡챔버를 이용하여 염소를 대상으로 맥문동(Liriope platyphylla)과 유기산 혼합물의 메탄 저감 효과를 평가하기 위해 수행하였다. 메탄 챔버의 회수율은 3%의 메탄 표준가스를 3L/min으로 30분 동안 주입하여 측정하였으며 회수율 결과는 99±5.4%로 나타났다. 회수율 측정 후 거세 Boer종 육용염소 8두(체중 46.6±7.77kg)를 공시하여 2×2 crossover design으로 동물실험을 수행하였다. 실험사료는 1) 대조구(CON); 시판 농후사료와 tall fescue, 2) 처리구(MIX); 맥문동과 유기산 제제가 함유된 농후사료와 tall fescue로 구성하였으며 건물기준으로 체중의 2%를 일일 2회 오전 8시와 15시 30분에 동일한 양으로 급여하였다. 실험사료에 10일, 메탄 챔버에는 3일 동안 적응시켰으며 메탄 발생량은 tunable diode laser absorption spectroscopy로 염소 두당 1일씩 측정하였다. 또한, 건물섭취량, 체중을 측정하였다. 실험 결과, CON과 MIX의 메탄 발생량(g/d)은 각각 24.48g/d와 22.68g/d로 나타났으며, 건물섭취량 기준 메탄 발생량은 각각 26.81g/kg DMI와 24.83g/kg DMI로 나타났다. 일일 메탄 발생량과 건물섭취량 기준 메탄 발생량에 유의적인 차이는 없었으나 CON보다 MIX에서 수치적으로 낮게 나타났으며, 첨가 농도를 다르게 하여 추가적인 실험이 필요할 것으로 판단된다. 또한, 메탄 호흡챔버는 반추동물의 장내 발효과정에서 발생하는 온실가스인 메탄 발생량을 효율적으로 측정할 수 있는 것으로 판단되나, 이를 효율적으로 운용하고 측정의 정확도를 제고하기 위해서는 다양한 분야의 전문가들과 공동연구가 필요할 것으로 사료된다.
Abstract
This study aimed to investigate the effect of Liriope platyphylla and organic acids on enteric methane mitigation in goats using an open-circuit simplified respiration chamber system. Methane recovery was evaluated by injecting 3% standard methane gas for 30 min at 3 L/min. The percentage of methane recovery from the four chambers was 99±5.4%. Following the recovery test, an animal experiment was conducted using eight castrated Boer goats (body weight 46.6±7.77 kg) using a 2×2 crossover design. Experimental diets were as follows: 1) Control (CON), commercial concentrate and tall fescue, and 2) Treatment (MIX), concentrate supplemented with L. platyphylla and organic acids and tall fescue. Goats were offered feed at 2% of body weight (dry matter basis) in equal portions twice daily at 8:00 and 15:30. The goats were adapted to the feed and methane chamber for 10 and 3 days, respectively. Methane emission was measured one day per goat using tunable diode laser absorption spectroscopy, and temperature and airflow measurements were used to estimate methane emissions. Dry matter intake (DMI), body weight, and methane emission were measured during each period. Methane production with CON and MIX was 24.48 and 22.68 g/d, respectively, and 26.81 and 24.83 g/kg DMI, respectively. Although the differences were not significant, the use of supplements resulted in a numerical reduction in methane in MIX compared with CON. Collaboration with experts in other areas, including various engineering departments, is imperative to measure methane emissions using a chamber system accurately.
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