하이브리드 스캐폴드의 간헐적인 기계적 자극에 대한 영향
The Effect of Intermittent Mechanical Stimulation on Hydrogel Scaffold
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Mechanical stimulation has been reported to improve cell growth in bone and cartilage regeneration. Various research groups have described their own bioreactors that stimulate cell-seeded scaffolds. We have developed a bioreactor capable of applying controlled compressive loading to a cell-encapsulated agarose hydrogel. To compare the effect of mechanical stimulation on agarose hydrogel, we used one control group (static mode) and five experimental groups. The results show that the intermittent_3sec conditions improve proliferation of cells better than the other conditions studied.
Abstract
Mechanical stimulation has been reported to improve cell growth in bone and cartilage regeneration. Various research groups have described their own bioreactors that stimulate cell-seeded scaffolds. We have developed a bioreactor capable of applying controlled compressive loading to a cell-encapsulated agarose hydrogel. To compare the effect of mechanical stimulation on agarose hydrogel, we used one control group (static mode) and five experimental groups. The results show that the intermittent_3sec conditions improve proliferation of cells better than the other conditions studied.
참고문헌 (13)
- Robert L. Mauck, "Functional Tissue Engineering of Articular Cartilage Through Dynamic Loading of Chondrocyte-Seeded Agarose Gels", Journal of Biochemical Engineering, 122(3), pp.252~260 (2000)
- John D. Kisiday, "Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffold", Journal of Biome-chanics, 37(5), pp.594~604 (2004)
- U. Meyer, "Design and performance of a bioreactor system for mechanically promoted threedimensional tissue engineering", British Journal of Oral and Maxillofacial Surgery, 44(2), pp.134~140 (2006)
- Z. Chunqiu, "Direct compression as an appropriately mechanical environment in bone tissue reconstruction in vitro", Med. Hypothese, 67(6), pp.1414~1418 (2006)
- Hunter, C. J., "Dynamic compression of chondrocyte-seeded fibrin gels : effects on matrix accumulation and mechanical stiffness", Osteo Arth-ritis and Cartilage, 12(2), pp.117~130 (2004)
- Orahn Preiss-Bloom, "Real-time monitoring of force response measured in mechanically stimulated tissue-engineered cartilage", Artificial Organs, 33(4), pp.318~327 (2009)
- Taly P. Appelman, "The differential effect of scaffold composition and architecture on chondrocyte response to mechanical stimulation", Biomaterials, 30(4), pp.518~525 (2009)
- G. E. Nugent-Derfus, "Continuous passive motion applied to whole joints stimulates chondrocyte biosynthesis of PRG4", Osteo Arthriti-sand Cartilage, 15(5), pp.556~574 (2007)
- S. -J. Lee, "Development of a hybrid scaffold and a bioreactor for cartilage regeneration", Chinese Sci. Bulletin, 54(19), pp.3608~3612 (2009)
- Awad, H. A., "Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate and gelatin scaffolds", Biomaterials, 25(16), pp.3211~3222 (2004)
- H. Towbin, "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications", Proc. Natl. Acad. Science, 76(9), pp.4350~4354 (1979)
- 이승재, "Performance Analysis of Scaffold Fabrication System Based on Micro-stereolithography Technology", 한국기계기술학회지, 14(6), pp.11~16 (2012), 한국기계기술학회
- 이승재, "광섬유를 이용한 미세 광 기계식 가속도 센서의 개발", 한국기계기술학회지, 13(4), pp.93~100 (2011), 한국기계기술학회