The Synergistic Effects of Agarose Scaffold Supplemented with Low-molecular-weight Silk Fibroin in Bone Tissue Regeneration
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초록
Silk protein and agarose are widely known as biocompatible materials in the human body. A three-dimensional (3D) scaffold composed of agarose and lowmolecular-weight silk fibroin (LSF) was fabricated and examined in terms of structural characteristics and cellular responses in bone tissue engineering. This study showed that mouse pluripotent precursor cells attached to and proliferated uniformly on and within the LSF-containing 3D scaffold. Interestingly, cell proliferation and attachment was shown to be higher in a 3D scaffold containing 0.02% LSF, as compared to other LSF concentrations. The results of this study suggest that agarose-LSF scaffolds may be useful materials for tissue engineering.
Abstract
Silk protein and agarose are widely known as biocompatible materials in the human body. A three-dimensional (3D) scaffold composed of agarose and lowmolecular-weight silk fibroin (LSF) was fabricated and examined in terms of structural characteristics and cellular responses in bone tissue engineering. This study showed that mouse pluripotent precursor cells attached to and proliferated uniformly on and within the LSF-containing 3D scaffold. Interestingly, cell proliferation and attachment was shown to be higher in a 3D scaffold containing 0.02% LSF, as compared to other LSF concentrations. The results of this study suggest that agarose-LSF scaffolds may be useful materials for tissue engineering.
참고문헌 (24)
- Aymard P, "Influence of thermal history on the structural and mechanical properties of agarose gels", Biopolymer, 59, pp.131~144 (2001)
- Cao Y, "Biodegradation of Silk Biomaterials", Int. J. Mol. Sc, 10, pp.1514~1524 (2009)
- Chun C, "The use of injectable, thermosensitive poly(organophosphazene)-RGD conjugates for the enhancement of mesenchymal stem cell osteogenic differentiation", BIOMATERIALS, 30, pp.6295~6308 (2009), ELSEVIER SCI LTD
- Edelman ER, "Perivascular and intravenous administration of basic fibroblast growth factor: vascular and solid organ deposition", Proc Natl Acad Sci USA, 90, pp.1513~1517 (1993)
- Engelhardt E, "A collagen-poly(lactic acid-co-3- caprolactone) hybrid scaffold for bladder tissue regeneration", Biomaterials, 32, pp.3969~3976 (2011)
- Huang G, "Proteomic analysis of 3T3-L1 preadipocytes having a higher cell proliferation rate after treatment with low-molecularweight silk fibroin peptides", Cell Prolif, 43, pp.515~527 (2010)
- Kang SW, "The effect of conjugating RGD into 3D alginate hydrogels on adipogenic differentiation of human adipose-derived stromal cells", Macromol Biosci, 11, pp.673~679 (2011)
- Kearns V, "Silk based Biomaterials for Tissue Engineering", Topics in Tissue Engineering, 4, pp.1~19 (2008)
- Jwa-Young Kim, "Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells", BMB reports, 43(1), pp.52~56 (2010), 한국생화학분자생물학회
- Koch MA, "Perfusion cell seeding on large porous PLA", calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters, 2010, pp.95, 1011~1018
- Lai Y, "Design and synthesis of a potent peptide containing both specific and nonspecific cell-adhesion motifs", Biomaterials, 31, pp.4809~4817 (2010)
- Lee EH, "A combination graft of low-molecularweight silk fibroin with Choukroun platelet-rich fibrin for rabbit calvarial defect", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 109, pp.e33~e38 (2010)
- Meinel L, "The inflammatory responses to silk films in vitro and in vivo", Biomaterials, 26, pp.147~155 (2005)
- Park JH, "Microporous Cell-Laden Hydrogels for Engineered Tissue Constructs", BIOTECHNOLOGY AND BIOENGINEERING, 106(1), pp.138~148 (2010), JOHN WILEY SONS INC
- Rahfoth B, "Transplantation of allograft chondrocytes embedded in agarose gel into cartilage defects of rabbits", Osteoarthritis Cartilage, 6, pp.50~65 (1998)
- Santin M, "In vitro evaluation of the inflammatory potential of the silk fibroin", J Biomed Mater Res, 46, pp.382~389 (1999)
- Shin HS, "Osteogenic differentiation of rat bone marrow stromal cells cultured on Arg-Gly-Asp modified hydrogels without dexamethasone and β-glycerolphosphate", Biomaterials, 26, pp.3645~3654 (2005)
- Stokolsa S, "Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury", Biomaterials, 27, pp.443~451 (2006)
- Sun L, "A 3D cartilage e Inflammatory cell culture system for the modeling of human Osteoarthritis", Biomaterials, 32, pp.5581~5589 (2011)
- Tai BC, "Modified polyelectrolyte complex fibrous scaffold as a matrix for 3D cell culture", Biomaterials, 31, pp.5927~5935 (2010)
- Taylor MS, "Six bioabsorbable polymers: in vitro acute toxicity of accumulated degradation products", J Appl Biomater, 5, pp.151~157 (1994)
- Uludag H, "Technology of mammalian cell encapsulation", Adv Drug Deliv Rev, 42, pp.29~64 (2000)
- Wang X, "Growth Factor Gradients via Microsphere Delivery in Biopolymer Scaffolds for Osteochondral Tissue Engineering", J Control Release, 134, pp.81~90 (2009)
- Xie D, "A novel route for the production of chitosan", poly (lactide-co-glycolide) graft copolymers for electrospinning, 2010, pp.5, 1~9
