Pancreatic Lipase의 활성 조절을 위한 약용식물 탐색
Study of Medicinal Plants for Regulation of Pancreatic Lipase Activity
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초록
천연물로부터 비만 치료에 효능이 있는 물질을 선별 하고자, 우리나라에서 민간의약으로 사용되어 온 한국전통약용식물로 부터 췌장의 지질분해효소인 리파제(lipase)의 활성을 저해시키는 약용식물을 탐색하였다. 131종의 전통 약용식물로 부터 33종의 약용식물 추출물들이 in vitro 상에서 유의적으로 췌장 리파제 활성을 저해시키는 효과를 나타내었다. 4종의 약용식물, 빈랑, 대황, 와송과 금모구척의 메탄올 추출물들이 in vitro 상에서 100㎍/ml의 농도로 처리했을시 약 60% 이상의 높은 효소활성 저해효과를 보였다. 실제로 대황은 72%의 가장 높은 활성을 나타내었고, 금모구척이 70.2%로 그 뒤를 따랐다. 이 활성은 양성대조군으로 사용된 Orlistat를 0.495 ㎍/ml의 농도로 처리한 결과보다 높은 활성이다. 다른 6종의 약용식물(봉출, 천초, 지유, 율금, 인진, 뇌환) 메탄올 추출물들 또한 40% 이상의 췌장 리파제 저해능을 보여주었다. 이미 많은 약용식물 추출물들의 췌장 리파제 저해활성들이 선행된 연구자들에 의해보고 되었다. 하지만 전체적인 약용식물의 종류와 규모에 비해서 연구된 부분은 아직 미미하다. 그러므로 본 연구의 결과들은 약용식물로부터 항비만 제재를 연구하기 위한 기본 자료로서 중요한 정보를 제공하게 될 것이다.
Abstract
For the development of the anti-obesity compounds from natural sources, the tests was carried out with Korean Traditional Medicinal Plants (KTMP) by inhibitory activity of pancreatic lipase enzyme assay with porcine pancreatic lipase (type VI-S, porcine pancreas from Sigma). Among the 131 Korean traditional medicinal plants, 33 KTMP extracts exhibited a significant inhibitory activity against pancreatic lipase in in vitro study. The methanol extracts of four KTMP namely, Areca catechu, Rheum undulatum, Orostachys japonicus, and Cibotium barometz, exhibited strong in vitro anti-lipase activity above 60% at the 100㎍/ml concentration. Particularly, the methanol extract of Rheum undulatum showed highest pancreatic lipase inhibitory activity as 72.0% followed by Cibotium barometz (70.2%) which were higher than orlistat at the 0.495㎍/ml concentration as positive control. The other 6 kinds of KTMP (Curcuma zedoaria, Rubia akane, Sanguisorba officinalis, Curcuma longa Linn, Artemisia scoparia and Omphalia lapidescens) also showed the over 40% of pancreatic lipase inhibitory activity. To the best of our knowledge, many KTMP have been screened for their pancreatic lipase inhibitory activity in the previously report. However, it’s too small study compared to the entire KTMP. Therefore, our results provide the more valuable information for studied the anti-obesity compounds from Korean traditional medicinal plants.
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