마우스에서 전기자극 스트레스의 면역조절 효과

Abstract

To explore the molecular mechanisms by which electric footshock has immunomodulatory effects, the effects of electric footshock, on the humoral and cell-mediated immune responses of ICR mice and then the gene expression of cytoWnes in splenocytes, including inter!eukin-1(IL-1), IL-2, IL-6, IL-12, the migration inhibitory factor(MIF), and the granulocyte macrophage-colony stimulating factor(GM-CSF), were investigated. Mice were subjected to. electric footshock for 3 daysftwo sessions a day, 11 footshocks a 31-mm session). The humoral response was measured using ICR mice immunized with rat RBC. The cell-mediated immune response was evaluated by contact hypersensitivity to 2,4-dinitrofluorobenzene(DNFB) and by phytohemagg!utin(PHA)-stimu!ated splenocytes proliferation assay. In the stressed group, electric footshock suppressed significantly the anti-rat RBC antibody(P<0.05), but enhanced significantly T&laquo; relative to T24 in contact hypersensitivity(PO.01) and T-cell proliferation response(P<0.05) by PHA stimulation relative to the control group. The gene expression of IL-2 and IL-6 were suppressed significantly in PHA-stimulated splenocytes. but that of IL-1, IL-12, GM-CSF and MIF were not. In conclusion, suppressed IL-6 gene expression seems to account for suppressed humoral immune response. In spite of enhanced cell-mediated immune response, the result is that IL-2 gene expression was suppressed, which suggests the two putative mechanisms. Firstly, while in the stressed group production of IL-2 occurs rapidly and thereafter declines abruptly, in the control group that of IL-2 occurs slowly and thereafter declines gradually. Secondly, there may be certain detourmg circuits extending the signal conduction system by IL-2 except the usual signal conduction system.