물윗수염박쥐(Myotis daubentinii ussuriensis)의 정자미세구조
Fine Structure of the Sperm in the Myotis daubentonii ussuriensis
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
본 연구는 물윗수염박쥐(Myotis daubentonii ussuriensisi)의 정자 미세구조적 특징을 알아보기 위하여 투과전자현미경(TEM)으로 관찰한 결과는 다음과 같다. 물윗수염박쥐 정자두부의 길이는 4.5 μm, 폭은 2.0 μm이었으며, 핵은 그 중 4.3 μm로 정자두부의 대부분을 차지하고 있었으며, 핵의 후방부에서 전방부쪽으로 갈수록 폭이 가늘어지며 두부의 전체적인 모양은 탄환형(bull-shaped)을 취하고 있었다. 또한,핵과 첨체는 Apical body에 의해 분리되어져 있었다. 정자경부는 basal plate, capitulum, segmented columns으로 구성되어져 있었다. 12~14개의 가는띠로 구성된 Segmented columns들의 밑부분은 중편부의 외측섬유와 연결되어져 있었다. 중편부의 미토콘드리아 초들은 나선상으로 배열되어져 있었으며, 그 수는 57개이었다. Satellite fibers는 외측섬유들 사이에 불규칙하게 관찰되어 졌으며, 중편부를 제외하고 다른 부분에서는관찰되지 않았다. 중편부의 외측섬유는 1, 5, 6, 9번이 다른 것보다 크며, 꼬리의 축사구조는 전형적인 9+2구조이었으며, 미부의미세소관들은 불규칙하게 배열되어져 있었다.
Abstract
The fine structures of the sperm morphology in the Myotis daubentonii ussuriensis were observed by transmission electron microscope. The results showed that the sperm head revealed bullet shaped, the width was showed a slender more than toward the posterior region to anterior region of nucleus. The sperm head was about 4.5 μm in length, being about 2.0 μm in width. The nuclear length was 4.3 μm, occupied most of the sperm head. The nucleus and acrosome were separated by the apical body. The neck region was composed the basal plate, capitulum and segmented columns. The segmented columns were about 12 to 14 in number and connected with the outer dense fibers of the middle piece. The mitochondria sheath were arranged like the thread of a screw, and the total number of mitochondrial gyres were 57. The satellite fibers were observed irregularly among the outer dense fibers in the middle piece. Except the middle piece they are not observed in the principal and end pieces of the tail. In general, the tail show axoneme composed of a 9+2 microtubular pattern, and microtubules of the end piece were arranged irregularly.
참고문헌 (14)
- Breed WG, "Sperm morphology of murid rodents from Malaysia and its possible phylogenetic significance", Am Mus Nat Hist, 2856, pp.1~12 (1986)
- Cho JH, "Ultrastructure study on the spermatogenesis of pale chub (Zacco platypus)", Kor J Electr Micros, 28, pp.181~191 (1998)
- Fawcett DW, "The fine structure of the bat spermatozoa", Am J Anat, 116, pp.567~610 (1965)
- Hamasaki M, "Three-dimensional structures of the neck region of the hamster spermatozoa in the caudal Epididymis", Arch Histol Cytol, 57, pp.59~65 (1994)
- Kim SS, "Morphological comparison of spermatozoa in the Korean Greater Horseshoe Bat Rhinolophus ferrumequinum korai and long-fingered bat Miniopterus Schreibersi fuliginosus", Korean J Electr Micros, 29, pp.1~10 (1999)
- 이정훈, "큰발웃수염박쥐(Myotis macrodactylus)의 정상피세포의 분화와 미세구조", 한국현미경학회지, 33(1), pp.3~39 (2003), 한국현미경학회
- M-ori T, "Comparative sperm structure in bats (Chiroptera): Some taxonomic and adaptive implications", Mém Mus natn Hist nat, 166, pp.421~429 (1995)
- Nicander L, "Fine structure of boar spermatozoa", Z Zellforsch, 57, pp.390~405 (1962)
- Oh YK, "Spermiogensis in the Japanese greater horseshoe bat, Rhinolophus ferrumquinum nippon", J Fac Agr Kyushu Univ, 29, pp.203~209 (1985)
- Son SW, "Spermtogenesis in the korean daubenton’sbat Myotis daubentonii ussuriensis", Dev Reprod, 1, pp.9~24 (1997)
- Stefanini M, "Fine structure of the connecting piece of the spermatozoon", Proc. 25th Ann. Meeting Electr. Micro. Soc.Amer, pp.196~197 (1967), Claitor’s Book Store
- Van deurs B, "Ultrastructure of the spermtozoa of the Teleost Pantodon buchholzi with particular reference to the midpiece", J Ultrastr Res, 42, pp.517~533 (1972)
- Yanagimachi R, "Fine structure of the hamster sperm head", Am J Anat, 128, pp.367~388 (1970)
- Zamboni L, "The fine structure of the neck of mammalian spermatozoa", Anat Rec, 169, pp.155~172 (1970)