자초(Lithospermum erythrorhizon) 추출물이 UVB로 조사된 생쥐 피부에 미치는 영향
The Effect of Lithospermum erythrorhizon Extracts in UVB-Irradiated Mouse Skin
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was intended to identify the effectiveness of Lithospermum erythrorhizon in the UVB-irradiated mouse skin. The C57BL mice were divided into three groups; the control group, the UVB irradiated group (UVB group), and the group treated with Lithospermum erythrorhizon extracts after UVB irradiation (UVB+Le group). 10 mouses were collected and sacrificed at 24 hrs, 48 hrs, 72 hrs, 120 hrs, and 168 hrs, respectively. In the result, the transepidermal water loss (TEWL) was decreased the UVB+Le group than UVB groups by time. At the 168 hrs group was significantly lower (p⁄0.05). In the result, the melanin value was decreased in the UVB+Le group than UVB group, but meaningless (p¤0.05). In the result of erythema index, the UVB+Le group was meaningfully lower at 24 hrs, 48 hrs, and 72 hrs group than UVB group (p⁄0.05). In the result of scanning electron micrograph observation, the UVB+Le group was allevited swelling than UVB group at the 24 hrs, formation of the scab at the 48 hrs, regular plate shap at the 72 hrs, new keratin observated at the 120 hrs partially, and fine fiber covered epidermis surface at the 168 hrs. In the result of transmission electron micrograph observation, the UVB+Le group was facilitation of increased lamellar bodies and reformation lamellar bodies than UVB group at the all groups. Almost all the structures were recovered at the 168 hrs group. In conclusion, Lithospermum erythema extracts may recovery on the UVB-irradiated mouse skin.
Abstract
This study was intended to identify the effectiveness of Lithospermum erythrorhizon in the UVB-irradiated mouse skin. The C57BL mice were divided into three groups; the control group, the UVB irradiated group (UVB group), and the group treated with Lithospermum erythrorhizon extracts after UVB irradiation (UVB+Le group). 10 mouses were collected and sacrificed at 24 hrs, 48 hrs, 72 hrs, 120 hrs, and 168 hrs, respectively. In the result, the transepidermal water loss (TEWL) was decreased the UVB+Le group than UVB groups by time. At the 168 hrs group was significantly lower (p⁄0.05). In the result, the melanin value was decreased in the UVB+Le group than UVB group, but meaningless (p¤0.05). In the result of erythema index, the UVB+Le group was meaningfully lower at 24 hrs, 48 hrs, and 72 hrs group than UVB group (p⁄0.05). In the result of scanning electron micrograph observation, the UVB+Le group was allevited swelling than UVB group at the 24 hrs, formation of the scab at the 48 hrs, regular plate shap at the 72 hrs, new keratin observated at the 120 hrs partially, and fine fiber covered epidermis surface at the 168 hrs. In the result of transmission electron micrograph observation, the UVB+Le group was facilitation of increased lamellar bodies and reformation lamellar bodies than UVB group at the all groups. Almost all the structures were recovered at the 168 hrs group. In conclusion, Lithospermum erythema extracts may recovery on the UVB-irradiated mouse skin.
참고문헌 (51)
- Ahn SK, "Barrier rcovery after topically applied desoxymethasone ontment, vaseline and hydrobase on benzalkonium chloride-irritated hairless mice skin", Korean J Dermatol, 36(5), pp.820~826 (1998)
- Alan N, "Stratum corneum moisturization at the molecular level", J Invest Deramatol, 103, pp.731~774 (1994)
- Bae JH, "long-term comparative study of erythema and pigmentation induced by UVA-1, broad band UVA, narrow band UVB, and broad band UVB" (2002), Kosin University
- Bouwstra JA, "The lipid oranganisation in the skin barrier", Acta Derm Venereol supp, 208, pp.23~30 (2000)
- 조광현, "광노화에 따른 피부의 조직학적 변화", 대한피부과학회지, 41(6), pp.754~760 (2003), 대한피부과학회
- Cho MH, "Physical stability of shikonin derivates from the roots of lithospermum erythrorhizon cultivated in Korea", J Aeric Food Chem, 47, pp.4117~4120 (1999)
- Cho YH, "Viability of cultured human keratinocyte and melanocyte after UVB exposure", Korean J Dermatol, 35(2), pp.258~265 (1997)
- Choi TJ, "The effect of solvents polarity on the skin barrier in hairless mice", Korean J Dermatol, 35(4), pp.660~666 (1997)
- Elias PM, "Mammalian epidermal barrier layer lipids: Composition and influence on structure", J Invest Deramatol, 69, pp.355~546 (1977)
- Fartasch M, "Structural relationship between epidermal lilid lamellae, lamellar bodies and desmosomes in human epidermis: an ultrastructural study", Brit J Dermatol, 128, pp.1~9 (1993)
- Feingold KR, "Permeability barrier homeostasis: Its biochemical basis and regulation", Cosmetic & Toiletries, 112, pp.49~59 (1997)
- Hahm JH, "A study on the morphological changes of stratum corneum in psoriatic patient", Kor Res Inst Better Living, 50, pp.165~172 (1992)
- Hisa T, "Shikonin, an ingredient of Lithospermum erythrorhizon, inhibits angiogenesis in vivo and in vitro", Anticancer Res, 18(2A), pp.783~790 (1998)
- Hou Y, "Effect of shikonin on human breast cancer cells proliferation and apoptosis in vitro", Yakugaku Zasshi, 12, pp.1383~1386 (2006)
- Hwang SY, "Isolation and quantitative analysis of acetylshikonin from Lithospermi Radix", Kor J Pharmacogn, 31(3), pp.259~299 (2000)
- Imokawa G, "Importance of intracellular lipids in water-retention properties of the stratum corneum: induction and recovery study of surfactant dry skin", Arch Dermatol Res, 281, pp.45~51 (1989)
- Johanna THM, "Laser Surg Med", 34, pp.141~145 (2004)
- Kang JH, "The anti-inflammatory effect of Lithosperimi Radix and suppression of iNOS and TNF-alpha production" (2005), Kyunghee University
- Kim AN, "Anticancer effect of Lithospermum erythrorhizon against L1210 cell in relation with Catalse, Catalse, SOD & GPx" (1999), Sangmeong University
- Kim H, "Antitumor effects of acetylshikonine and some synthesized naphthazarins on L1210 and S-180 systems", Yakhak Hoeji, 34(4), pp.262~266 (1990)
- Nga mice, "Dietary effect of Lithospermum erythrorhizon Siebold & Zuccarini on ceramides and natural moisturizing factor in the skin of NC", pp.2006, Kim JM
- 김진숙, "야생 및 재배 지치뿌리의 Shikonin 화합물 확인", 한국식품영양과학회지, 35(2), pp.177~181 (2006), 한국식품영양과학회
- Kim MN, "Studies on the constituents of Lithospermum erythrorhizon", J Sun Moon University Natural Science, 4, pp.25~39 (2001)
- Kim SH, "Effects of the Lithospermum erythrorhizon on the atopic dermatitis", Association Korean Oriental Pediatrics, 18, pp.63~75 (2004)
- Kim SY, "Effect of Lithospermi Radix on the allergic inflammatory response" (2006), Kyunghee Universsity
- Kim YC, "The effect of repeated ultraviolet irradiation on epidermal melanocyte of the expossed and shielded ear in C57BL mice", Korean J Dermatology, 26(3), pp.283~291 (1988)
- Kim YK, "The effect of UV-B radiation on epidermal melanocytes of C57BL mouse", Korean J Dermatol, 26(2), pp.139~144 (1988)
- Landmann L, "Epidermal permeability barrier: transformation of lamellar granule-disks into intercellular sheets by a freezefracture study", J Invest Deramato, 87(2), pp.202~209 (1986)
- 진종언, "지치(Lithospermum erythrorhizon) 추출물의 멜라닌 생합성 억제효과", 한국식품영양과학회지, 34(9), pp.1325~1329 (2005), 한국식품영양과학회
- 이해준, "자외선 B 조사 마우스에서 피부손상에 대한 홍삼의 효과", 고려인삼학회지, 30(4), pp.194~198 (2006), 고려인삼학회
- Lee HJ, "The morphological observation on the cutaneous wound healing of mouse", Chungnam Medical Journal, 16(1), pp.29~37 (1989)
- 이진욱, "장기간 및 고용량의 전신 스테로이드 투여가 hairless mice의 피부장벽에 미치는 영향", 대한피부과학회지, 42(3), pp.281~289 (2004), 대한피부과학회
- Lee SH, "The effects of a single exposure of UVB on morphologic changes and proliferation in cultured normal melancocytes" (1992), Kyunghee University
- Lee SH, "Skin Barrier" (2004), Ryomoongak
- Lee SH, "Skin barrier", Korean J Dermatol, 37, pp.825~837 (1999)
- 임상희, "저용량 UVA1 요법으로 치료한 국한성 경피증 1예", 대한피부과학회지, 44(4), pp.474~478 (2006), 대한피부과학회
- 양재헌, "화상 및 창상에 대한 자근·치자 복합제제의 경피 흡수 및 치료효과", Journal of Pharmaceutical Investigation, 35(4), pp.255~263 (2005), 한국약제학회
- Ministry for Health welfare, "Development of Gromwell as a functional health food for skin mositurizing improvement and treatment of atopic dermatitis" (2006)
- Park HJ, "Effect of ginseng Alkaloid on epidermal melanocytes in UVB-Irradiated black mice" (1998), Ehwa Womens University
- Park SB, "Erythema and melanin index according to UVB radiation doses by use of a reflectance spectrophotometer in Korean young adults", Korean J Dermatol, 35(4), pp.729~735 (1997)
- Pathak MA, "Free radical in human skin before and after exposure to light", Arch Biochem Biophys, 123, pp.468~476 (1968)
- Rawlings AV, "Stratum corneum moisturization at the molecular level", J Invest Deramatol, 103, pp.731~740 (1994)
- Rhodes AR, "Sun-induced freckles in children and young adults", Cancer, 67, pp.1990~2001 (1991)
- Singh F, "Shikonin modulates cell proliferation by inhibiting epidermal growth factor receptor signaling in human epidermoid carcinoma cells", Cancer Lett, 200, pp.115~121 (2003)
- Staniforth V, "Shikonins, phytocompounds from Lithospermum erythrorhizon, inhibit the transcriptional activation of human tumor necrosis factor α promoter in vivo", J Bio Chem, 279, pp.5877~5855 (2004)
- Van der Valk PGM, "A functional study of the skin barrier to evaporative water loss by means of repeated cellophane-tape stripping", Clinical and Experimental Dermatol, 15, pp.180~182 (1990)
- Wertz PW, "The role of the corneocyts lipid envelopes in cohesion of the stratum corneum", J Invest Deramatol, 93, pp.169~172 (1989)
- Yoon DH, "The effect of ultraviolet B irradiation on the skin barrier function in hairless mice-the change of transepidermal water loss and stratum corneum lipids compostion", Kor J Dermatol, 33(4), pp.669~678 (1995)
- Yoon KJ, "Studies on the constitutements and their antibacterial effect of the root of Lithospermum erythrorhizon Sieb. et Zucc", Bul K H Pharm Sci, 16, pp.155~161 (1988)
- Youn JI, "Photomedicine", pp.110~116 (1994), Ryomoongak
- Zhai H, "Occlusion vs. skin barrier function", Skin Research and Technology, 8, pp.1~6 (2002)