치자 추출물의 염증 및 피부장벽 손상 완화효과
Alleviating Effect of Gardeniae fructus Water Extract on Inflammation and Skin-Barrier Damage
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초록
To investigate the inhibitory effects of Gardeniae fructus water extract on the inflammation and skin barrier damage, both the irradiation of UVB and the application of squalene monohydroperoxide (Sq-OOH) were applied to the backs of experimental animals for 4 weeks. And at the same time experimental materials were applied topically. Six to seven weeks female SKH-1 hairless mice were divided into five groups including normal (N; saline), control (C; UVB+Sq-OOH+ saline), vehicle control (VC; UVB+Sq-OOH+vehicle), positive control (PC; UVB+Sq-OOH+0.01% retinoic acid) and experimental (E; UVB+Sq-OOH+Gardeniae fructus water extract) groups, five animals each group. The skin erythema indices for PC and E groups were lower than that of C group. Lipid and water capacities for PC and E groups were higher than those of C group. Epidermis and dermis of C group were remarkably thickened and many inflammatory cells including lymphocytes, neutrophils and macrophages were found compared to N group. Whereas a few inflammatory cells were found in PC and E groups compared to C group. In comparison with C group, PC and E groups showed a relatively well preserved lipid lamellae in epidermis and a relatively much less infiltration of mast cells in dermis or hypodermis. As for the both absolute and relative weight of the spleen, PC group were significantly higher than the other groups. Therefore, Gardeniae fructus water extract could have a considerable inhibitory efficacy on the inflammation and skin barrier damage induced by UVB irradiation and Sq-OOH application.
Abstract
To investigate the inhibitory effects of Gardeniae fructus water extract on the inflammation and skin barrier damage, both the irradiation of UVB and the application of squalene monohydroperoxide (Sq-OOH) were applied to the backs of experimental animals for 4 weeks. And at the same time experimental materials were applied topically. Six to seven weeks female SKH-1 hairless mice were divided into five groups including normal (N; saline), control (C; UVB+Sq-OOH+ saline), vehicle control (VC; UVB+Sq-OOH+vehicle), positive control (PC; UVB+Sq-OOH+0.01% retinoic acid) and experimental (E; UVB+Sq-OOH+Gardeniae fructus water extract) groups, five animals each group. The skin erythema indices for PC and E groups were lower than that of C group. Lipid and water capacities for PC and E groups were higher than those of C group. Epidermis and dermis of C group were remarkably thickened and many inflammatory cells including lymphocytes, neutrophils and macrophages were found compared to N group. Whereas a few inflammatory cells were found in PC and E groups compared to C group. In comparison with C group, PC and E groups showed a relatively well preserved lipid lamellae in epidermis and a relatively much less infiltration of mast cells in dermis or hypodermis. As for the both absolute and relative weight of the spleen, PC group were significantly higher than the other groups. Therefore, Gardeniae fructus water extract could have a considerable inhibitory efficacy on the inflammation and skin barrier damage induced by UVB irradiation and Sq-OOH application.
참고문헌 (20)
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