경골어류 시클리드과 Cichlasoma managuensis의 수정란 난막 미세구조
Ultrastructure of the Fertilized Egg Envelope in Cichlasoma managuensis, Cichlidae, Teleost
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초록
경골어류 시클리드과에 속하는 Cichlasoma managuense의 수 정란 난막구조를 광학현미경과 전자현미경을 이용하여 관찰하였 다. 수정란은 약황색의 불투명한 부착성 및 침란성란으로 장타원 형이었으며 크기는 장축 1.43±0.04 mm, 단축 1.85±0.03mm였 다. 수정란의 동물극쪽에서 수정을 위한 정자의 통로로 생각되는 난문이 위치하고 있었고 위란강은 잘 발달되어 있지 않았다. 수 정란 난막의 표면에는 부착성 망상구조물들이 덮고 있었고 난문 의 형태는 깔때기 형태였다. 수정란 난막의 단면을 관찰한 결과 2층으로 구성되어 있었으며 외층은 부착성 층, 내층은 전자밀도 가 서로 다른 13~15층의 층상구조를 하고 있었다. 수정란의 외형은 전형적인 시클리드과에 속하는 다른 어류와 동일한 형태를 하고 있어 과의 특성을 보이며 이들 수정란 난막 에서 표면과 단면의 미세구조는 Cichlasoma managuense만이 가 지고 있는 종특이성이 될 수 있기 때문에 경골어류의 종을 분류 하는데 형태학적 형질로 사용될 수 있을 것으로 사료된다.
Abstract
The ultrastructure of the fertilized egg envelope in Cichlasoma managuensis belonging to Cichlidae were investigated by routine light and electron microscopes. The fertilized eggs of Cichlasoma managuensis was of the light yellow, non-transparent, ellipsoidal, adhesive and nonfloted type. The size of fertilized egg was the major axis 1.92±0.08 mm, the minor axis 1.43±0.04 mm. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. An outer surface of fertilized egg envelope was covered by a adhesive reticular structures and the fertilized egg envelopes consisted of two distinct layers; an outer adhesive layer and an inner layer of 13~15 horizontal lower electron density lamellae alternating with interlamellae of higher electron density. The external shape of fertilized egg is common trait of fishes belonging to Cichlidae and these ultrastructural characters of fertilized egg envelope can be utilized in taxonomy of teleost.
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