홍차추출물 함유 누드팩의 Hairless 마우스 피부주름 형성 억제효과
Inhibitory Effects of Nude Pack Containing Black Tea Water Extract on Skin Wrinkle Formation in Hairless Mice
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홍차함유 누드팩의 피부주름 형성 억제효과를 알아보기 위해SKH-1 hairless 마우스 등 부위에 UVB를 5주간 조사하여 주름을 유발시키고 이와 병행하여 홍차함유 누드팩을 도포한 실험에서 다음과 같은 결과를 얻었다. (1) 육안적인 피부주름 양상 비교에서, 대조군은 정상군에 비해 주름 능선의 두께가 굵고 간격이넓으며 주름이 깊게 형성된 반면, retinoic acid를 도포한 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 주름 능선의 두께가 얇고 간격이 좁은 양상을 띠었으며 주름 총넓이 수치가 유의하게(p⁄0.001) 낮았다. (2) 피부의 조직학적 관찰에서, 대조군은 정상군에 비해 진피층내 교원섬유의 배열이 불규칙하고 양이 많이 줄어들었으며 변성된 탄력섬유가 군데군데엉겨 있는 탄력섬유증이 관찰된 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 상대적으로 교원섬유의 배열이 규칙적이고 밀도가 조밀하며 변성된 탄력섬유의 양이 줄어 있었다. (3) 피부조직의 MMP-3 mRNA 발현량 측정 결과, 정상군에비해 대조군은 유의하게(p⁄0.001) 높았으며 양성대조군과 홍차함유 누드팩 도포군은 대조군에 비해 유의하게(p⁄0.001) 낮았다. MMP-2와 MMP-9 단백질 활성 측정 결과, 대조군은 정상군에 비해 현저하게 높은 반면, 양성대조군과 홍차함유 누드팩 도포군은 대조군보다 현저하게 낮게 나타났다. 본 연구를 통하여,홍차함유 누드팩 도포군은 hairless 마우스에서 세포외 기질단백질의 분해 억제와 피부주름 형성 억제효과를 보였음을 확인하였다.
Abstract
The aim of this study was to evaluate the inhibitory effect of nude pack containing black tea water extract (NPBT) on skin wrinkle formation in hairless mice. Skin wrinkles were induced by UVB irradiation to the backs of hairless mice for 5 weeks. And at the same time, NPBT was applied topically. Wrinkle formation, histological changes, expression of matrix metalloproteinase-3 (MMP-3) and protein activities of MMP-2 and MMP-9 were observed or analyzed. Wrinkles for the control group were formed as a pattern of deep furrows and thick crests. Whereas wrinkles for the NPBT treated group were formed as a pattern of shallow furrows and thin crests, and their wrinkle areas were significantly (p⁄0.001)lower than the control group. Collagen fibers were arranged irregularly and sparse in density and some elastic fibers were degenerated in the control group, while they were almost intact in the NPBT treated group. MMP-3 mRNA expression in the control group was significantly (p⁄0.001) higher than the normal group, and that of NPBT treated group was significantly (p⁄0.001) lower than the control group. The NPBT treated group showed remarkably lower protein activities of MMP-2 and MMP-9 than the control group. NPBT could have a considerable inhibitory effect on skin wrinkle formation in hairless mice.
참고문헌 (26)
- Adler RR, "Expression of extracellular matrix-degrading metalloproteinase and metalloproteinases inhibitor is developmentally regulated during endoderm differentiation of embryonal carcinoma cells", Development, 110, pp.221~230 (1990)
- Boyd CD, "Mammalian tropoelastin: multiple domains of the protein define an evolutionary divergent amino acid sequence", Matrix, 11, pp.235~241 (1991)
- Fisher GJ, "Molecular basis of sun-induced premature ageing and retinoid antagonism", Nature, 379, pp.335~339 (1996)
- Gary JF, "Pathophysiology of premature skin aging induced by ultraviolet light", New Engl J Med, 337, pp.1419~1428 (1997)
- Cho KH, "Histological changes in the skin with innate ageing", Kor J Dermatol, 36(6), pp.971~980 (1998)
- Herron GS, "Secretion of metalloproteinases by stimulated capillary endothelial cells I: production of procollagenase and prostromelysin exceeds expression of proteolytic activity", J Biol Chem, 261, pp.2810~2813 (1986)
- 형순희, "Hairless 마우스에서 치자 열수추출물의 피부 주름형성 억제효과", 대한피부미용학회지, 7(2), pp.123~136 (2009), 대한피부미용학회
- Indik X, "Alternative splicing of human elastin mRNA indicated by sequence analysis of cloned genomic and complementary DNA", Proc Nat Acad Sci USA, 84, pp.5680~5684 (1987)
- Kähäri VM, "Deletion analyses of 5′-flanking region of the human elastin gene. Delineation of functional promoter and regulatory cis-elements", J Biol Chem, 265, pp.9485~9490 (1990)
- Karin SK, "Photoaging of the skin form phenotype to mechanisms. Exp Gerontol", 35, pp.307~316 (2000)
- Kim MH, "The antioxidative effects of the water-soluble extracts of plants used as tea materials", Kor J Food Sci Technol, 33(1), pp.12~18 (2001)
- 김영철, "Hairless 마우스에서 제라늄 오일의 피부노화 억제 효과(Ⅱ)", 한국미용학회지, 14(2), pp.504~513 (2008), 한국미용학회
- Kligman LH, "The hairless mouse model for photoaging", Clin Exp Dermatol, 14, pp.183~195 (1996)
- Leung LK, "Theaflavins in black tea and catechins in green tea are equally effective antioxidants", J Nutr, 131, pp.2248~2251 (2001)
- MacNaul KL, "Discoordinate expression of stromerylsin collagenase, and tissue inhibitor of metalloproteinases-1 in rheumatoid human synovial fibroblasts: synergistic effects of interleukin-1 and tumor necrosis factor-alpha on stromelysin expression", J Biol Chem, 265(28), pp.17238~17245 (1990)
- Obanda PM, "Changes in the chemical and sensory quality parameters of black tea due to variations of fermentation time and temperature", J Agri Food Chem, 75, pp.395~404 (2001)
- Obanda PM, "Changes in thearubigin fractions and theaflavin levels due to variations in processing conditions and their influence on black tea liquor brightness and total colour", J Agri Food Chem, 85, pp.163~173 (2004)
- 박숙경, "참당귀(Angelica gigas Nakai) 뿌리 추출물의 화장품소재 특성", Korean Chemical Engineering Research(HWAHAK KONGHAK), 47(5), pp.553~557 (2009), 한국화학공학회
- 유연승, "Scanning Electron Microscopic Observation of Human Skin Replica", 한국현미경학회지, 40(4), pp.267~270 (2010), 한국현미경학회
- Seite S, "Elastin change during chronological and photo-ageing: the important role of lysozyme", J Eur Acad Dermatol Venereol, 20(8), pp.980~987 (2006)
- Seo JY, "Skin aging from phenotype to mechanisms", Kor J Invest Dermatol, 8(4), pp.187~194 (2001)
- Shinji I, "Possible involvement of gelatinase in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse", J Invest Dermatol, 120, pp.1~7 (2003)
- Steinhoff M, "Inflammation, In Rook’s Textbook of Dermatology" (2004), Blackwell Science
- Svobodova A, "Natural phenolica in the prevention of UV-induced skin damage", Biom Papers, 147(2), pp.137~145 (2003)
- Varani J, "Inhibition of type 1 procollagen synthesis by damaged collagen in photoaged skin and by collagenase-degraded collagen in vitro", Am J Pathol, 158, pp.931~942 (2001)
- Wlaschek M, "Chronological ageing and photoageing of the fibroblasts and the dermal connective tissue", Clin Exp Dermatol, 26, pp.592~599 (2001)