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생쥐 생식소 및 배아의 프로케스테론 수용체 막성분 1과 2의 발현에 관한 연구

Expression of progesterone receptor membrane component 1 and 2 in the mouse gonads and embryos

이경아|김경화
한국발생생물학회 (The Korea Society Of Developmental Biology)|2007.06|발생과 생식 (DEVELOPMENT & REPRODUCTION)|제11권 제1호|pp. 21-29

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참고문헌

주제분류생물학

초록

본 연구진은 선행 연구에서 progesterone receptor membrane component 2 (pgrmc2) 유전자가 미성숙 난자에서 높게 발현하는 것을 발견하였다. 본 연구는 pgrmc2와 pgrmc1 유전자가 쥐의 생식소와 배아의 발달 단계에 따라 어떻게 발현하는지 알아 보기 위해 수행하였다. 이들 유전자는 난소, 정소, 배아, 그리고 난자의 다양한 발달 단계에서 발현하는 것을 알 수 있었다. 쥐 난자 세포막에 프로게스테론 수용체가 존재하는 것은 progesterone-3-O-carboxymethyl oxime-BSA-fluorescein isothiocyanate (P4-BSA-FITC)의 결합을 이용하여 확인하였다. 본 결과는 프로게스테론 수용체의 세포막 구성 요소들이 생쥐의 난자, 배아, 그리고 생식소에서 발현함을 최초로 확인한 연구결과다. 스테로이드 수용체의 세포막 구성 요소들의 기능, 특히 프로게스테론 수용체의 세포막 구성 요소들의 기능을 알아 보기 위해 추후 연구가 계속되어야 할 것이다.

Abstract

Previously, we found progesterone receptor membrane component 2 (pgrmc2) was highly expressed in germinal vesicle (GV) stage oocytes. The present study was conducted to characterize the expression of pgrmc2, as well as pgrmc1, in the mouse gonads and embryos according to their developmental stages. We found that these membrane components were expressed in ovaries, testes, and embryos at various developmental stages in addition to oocytes. Progesterone-3-O-carboxymethyl oxime-BSA-fluorescein isothiocyanate (P4-BSA-FITC) was applied to visualize the presence of the progesterone receptor on mouse oocyte membrane, and we confirmed that immobilized progesterone is localized at surface of the oocyte. This is, at our knowledge, the first report regarding the expression of membrane component of progesterone receptor in the mouse oocytes, embryos, and gonads. The function and signal transduction pathway of progesterone receptor membrane components in oocytes requires further studies.

참고문헌 (31)

  1. "Polyadenylated RNA of mouse ova and loss of maternal RNA in early development. Dev Bio 74", pp.1~8 (1980)
  2. "Plasma membrane mediated action of progesterone in amphibian", 109, pp.293~301 (1998)
  3. "Dynamic organization of DNA replication in one-cell mouse embryos relationship to transcriptional activation", 236, pp.201~211 (1997)
  4. "The initiation of follicle growth the oocyte or the somatic cells?", 187, pp.11~18 (2002)
  5. "Non-genomic progesterone receptors in the mammalian ovary", 125, pp.~15 (2003)
  6. "Expression and regulation of progestin membrane receptors in the rat corpus luteum.", 146, pp.5522~5532 (2005)
  7. "Biology: Concepts & Connections", pp.519~ (2003), Benjamin Cummings
  8. "Selective progesterone receptor modulators and progesterone antagonists mechanisms of action and clinical applications", 11, pp.293~307 (2005)
  9. "The mammalian oocyte orchestrates the rate of ovarian follicular development.", 99, pp.2890~2894 (2002)
  10. "Multiple actions of steroid hormones a focus on rapid, nongenomic effects", 52, pp.513~556 (2000)
  11. "Expression of progesterone receptors A and B in the mouse ovary during the estrous cycle", 145, pp.3487~2494 (2004)
  12. "In situ photolinked nuclear progesterone receptors of human breast cancer cells subunit molecular weights after transformation and translocation. Endocrinology 113" (1983)
  13. "Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B. EMBO J 9", pp.1603~1614 (1990)
  14. "A progesterone dependent step in ovulation induced by human chorionic gonadotrophin in immature rats primed with pregnant mare serum gonadotrophin. J Endocrinol 87" (1980)
  15. "Steroid action at the plasma membrane progesterone stimulation of phosphatidylcholine-specific phospholipase C following release of the prophase block in amphibian oocytes. Mol Cell Endocrinol. 92", pp.33~44 (1993)
  16. "Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes. J Exp Zool 177", pp.129~45 (1971)
  17. "Changes in mouse granulose cell gene expression during early luteinization", 146, pp.309~317 (2005)
  18. "Inhibition of ovulation in immature rats by anti-progesterone antiserum. J Endocrinol 73" (1977)
  19. "and functional activity of the progesterone receptor in granulosa cells of rat preovulatory follicles. Endocrinology 133" (1993)
  20. "Transient expression of progesterone receptor messenger RNA in ovarian granulosa cells after the preovulatory luteinizing hormone surge. Mol Endocrinol 5" (1991)
  21. "Expression and function of PAIRBP1 within gonadotropin-primed immature rat ovaries PAIRBP1 regulation of granulosa and luteal cell viability", 73, pp.261~270 (2005)
  22. "Multiplicity of progesterone's actions and receptors in the mammalian ovary", 75, pp.2~8 (2006)
  23. "Progesterone membrane receptor component 1 expression in the immature rat ovary and its role in mediating progesterone's antiapoptotic action", 147, pp.3133~3140 (2006)
  24. "Nongenomic actions of steroid hormones in reproductive tissues", 19, pp.3~17 (1998)
  25. "Role of steroid synthesis in the process of ovulation. Biol Reprod 10"
  26. "Gonadotrophin-induced gene regulation in human granulosa cells obtained from IVF patients modulation of steroidogenic genes cytoskeletal genes and genes coding for apoptotic signaling and protein kinases", 10, pp.299~311 (2004)
  27. "Molecular action of progesterone. Int J Biochem 22" (1990)
  28. "Isolation and characterization of a novel gene induced by 2 8-tetrachlorodibenzo-p-dioxin in rat liver", 17, pp.2609~2615 (1996)
  29. "The interaction of steroids with Rana pipiens oocytes in the induction of maturation. Dev Biol 25", pp.232~47 (1971)
  30. "Membrane-initiated steroid actions and the proteins that mediate them.", 220, pp.9~19 (1999)
  31. "Specific nongenomic actions of steroid hormones.", 59, pp.365~393 (1997)

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