Expression of Membrane Fusion Related Genes in Mouse Ovary
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Granulosa cells surround the oocyte within the ovarian follicle and play an essential role in creatingconditions required for oocyte as well as follicular development. The current study was conductedto examine the gene expression profile of mouse ovaries during the primordial to primary follicletransition process. Total RNAs from mouse ovaries on day 5 and day 12 were synthesized cDNAusing annealing control primers. The DEGs were cloned and their identities were analyzed byBLAST search. The Plekha5 and Anxa11 were highly expressed in primary follicle stage. Bycontrast, their expression was increased in granulosa cells at the primary follicle stage. We havesuccessfully discovered a list of genes expressed in day 5 and day 12 ovaries and confirmed thatsome of them are differentially expressed in PMF and/or PRI. This is a spatial-temporal regulatorymechanism on the ovarian folliculogenesis through membrane fusion. The gene expression profilefrom the current study would provide insight for future study on the mechanism(s) involved inprimordial-primary follicular transition. This will provide information for identification of themechanism of ovarian dysfunction.
Abstract
Granulosa cells surround the oocyte within the ovarian follicle and play an essential role in creatingconditions required for oocyte as well as follicular development. The current study was conductedto examine the gene expression profile of mouse ovaries during the primordial to primary follicletransition process. Total RNAs from mouse ovaries on day 5 and day 12 were synthesized cDNAusing annealing control primers. The DEGs were cloned and their identities were analyzed byBLAST search. The Plekha5 and Anxa11 were highly expressed in primary follicle stage. Bycontrast, their expression was increased in granulosa cells at the primary follicle stage. We havesuccessfully discovered a list of genes expressed in day 5 and day 12 ovaries and confirmed thatsome of them are differentially expressed in PMF and/or PRI. This is a spatial-temporal regulatorymechanism on the ovarian folliculogenesis through membrane fusion. The gene expression profilefrom the current study would provide insight for future study on the mechanism(s) involved inprimordial-primary follicular transition. This will provide information for identification of themechanism of ovarian dysfunction.
참고문헌 (16)
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