Postnatal Ontogeny of Expression of Monocarboxylate Transporters(MCTs) and Two Regulatory Proteins, Basigin and Embigin, in The Epididymis of Male Rat
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
본 연구는 생후 발달 과정 동안 monocarboxylate transporter(MCT) isoform과 MCT의 발현 조절 단백질로 알려진 basigin(Bsg)과 embigin의 mRNA 발현을 흰쥐의 부정소에서 부위별로 real-time PCR 방법을 사용하여 알아보았으며, 에스트로젠과 에스트로젠 수용체 α의 작용에 의해 MCT1 발현이 조절되는지를 알아보기 위해 estrogen receptor α knockout(αERKO) 마우스를 이용하여 immunohistochemistry 방법을 통해 탐구하였다. 본 연구 결과는 다양한 MCT isoform(MCT1, 2, 3, 4와 8), Bsg과 embigin의 mRNA 발현이 부정소의 부위별로 연령에 따라 다르게 나타나며, 부정소에서 MCT1 단백질 발현은 corpus와 caudal 부위에서 apical 지역에 한정되어 나타나는 것을 보여 주었다. 또한 부정소에서 MCT1 단백질 발현은 에스트로젠 수용체 α의 존재 여부와 상관 없음이 보여졌다. 따라서, 본 연구는 MCT가 남성 생식기관인 부정소에서 정자 성숙과 저장을 위한 적절한 환경을 형성함으로써 남성 생식력의 유지에 관여 할 수 있음을 시사한다. (색인어 : Epididymis, Monocarboxylate transporter, Basigin, Embigin)
Abstract
In the present study, real-time PCR was performed to evaluated expression of several isoforms of monocarboxylate transporters(MCTs) and two known MCT regulatory proteins, basigin (Bsg) and embigin, in the epididymis of the male reproductive tract during postnatal development. In addition, ERα-mediated regulation of MCT1 expression in the epididymis was determined with estrogen receptor(ER) α knockout(αERKO) mice by immunohistochemistry. Results from the current study demonstrated differential expression of MCT isoform(MCT 1, 2, 3, 4, and 8), Bsg, and embigin mRNAs in rat epididymis according to postnatal age and epididymal region. In addition, immunohistochemical study of MCT1 revealed the limited localization of MCT1 at apical area of corpus and caudal epididymis. The present study also showed that expression of MCT1 was not directly regulated by ERα. The findings from the current study suggest that MCTs would involve in establishing adequate microenvironment for sperm maturation and storage in the epididymis, eventually leading to maintenance of male fertility.
참고문헌 (25)
- Boussouar, F., "Developmental and hormonal regulation of the monocarboxylate transporter 2(MCT2) expression in the mouse germ cells", Biol. Reprod, 69, pp.1069~1078 (2003)
- Branuchi, S., "Kinetics, molecular basis, and differentiation of L-lactate transport in spermatogenic cells", Am. J. Physiol. Cell. Physiol, 288, pp.C523~C534 (2005)
- Brown, D., "Role of V-ATPase-rich cells in acidification of the male reproductive tract", J. Exp. Biol, 200, pp.257~262 (1997)
- Buyse, M., "Luminal leptin enhances CD147", MCT-1-mediated uptake of butyrate in the human intestinal cell line Caco2-BBE, 2002, pp.277, 28182~28190
- Cosentino, M. J., "Structure and function of the epididymis", Urol. Res, 14, pp.229~240 (1986)
- Cyr, D. G., "Structure and turnover of junctional complexes between principal cells of the rat epididymis", Microsc. Res. Tech, 30, pp.54~66 (1995)
- DaSilva, N., "Segmental and cellular expression of aquaporins in the male excurrent duct", Biochim. Biophys. Acta, 1758, pp.1025~1033 (2006)
- Deora, A. A., "Mechanisms regulating tissue-specific polarity of monocarboxylate trans- porters and their chaperone CD147 in kidney and retinal epithelia", Proc. Natl. Acad. Sci. USA., 통권 : 102, pp.16245~16250 (2005)
- Garcia, C. K., "Molecular characterization of a membrane trans- porter for lactate, pyruvate, and other mono- carboxylates: implications for the Cori cycle", Cell, 76, pp.865~783 (1994)
- Garcia, C. K, "cDNA cloning of MCT2, a second monocarboxylate transporter expressed in different cells than MCT1", J. Biol. Chem, 270, pp.1843~1849 (1995)
- Gondos, B, "Postnatal and pubertal development in the Sertoli cell", pp.115~ (1993), Cache River Press, Clearwater, U.S.A.
- Guenette, R. S, "Embigin, a developmentally expressed member of the immunoglobulin super family, is also expressed during regression of prostate and mammary gland", Dev. Biol, 155, pp.307~314 (1998)
- Halestrap, A. P, "The proton-linked monocarboxylate transporter(MCT) family: structure, function, and regulation", Biochem. J, 343, pp.281~299 (1999)
- Halestrap, A. P, "The SLC16 gene family-from monocarboxylate trans- porters(MCTs) to aromatic amino acid transporters and beyond", Eur. J. Physiol, 447, pp.619~628 (2004)
- Jervis, K. M, "Dynamic changes in gene expression along the rat epididymis", Biol. Reprod, 65, pp.696~703 (2001)
- 이기호, "생후 발달과정동안 남성 생식기의 Efferent Ductules에서 Monocarboxylate Transporters(MCTs)와 Basigin의 발현 양상과 에스트로젠 수용체 α에 의한 MCT1 발현 조절 연구", 한국동물자원과학회지, 49(3), pp.309~320 (2007), 한국동물자원과학회
- Nakai, M., "Tissue distribution of basigin and monocarboxylate transporter 1 in the adult male mouse: a study using the wild-type and basigin gene knockout mice", Ana. Rec. A. Discov. Mol. Cell. Evol. Biol, 288, pp.527~535 (2006)
- Philp, N. J., "0Mouse MCT3 gene is expressed preferentially in retinal pigment and choroids plexus epithelia", Am. J. Physiol, 280, pp.C1319~C1326 (2004)
- Philp, N. J., "Loss of MCT1, MCT3, and MCT4 expression in the retinal pigment epithelium and neural retina of the 5A11", basigin-null mouse, 2003, pp.44, 1305~1311
- Rodríguez, C., "Regulation of gene transcription in the epididymis", Reproduction, 122, pp.41~48 (2001)
- Toyama, Y., "Histological characterization of defective spermato- genesis in mice lacking the basigin g", Anat. Histol. Embryol, 28, pp.205~213 (1999)
- Turner, T. T, "On the epididymis and its function", Invest. Urol, 16, pp.311~321 (1979)
- Vavaiya, K. V., "Testicular regulation of neuronal glucose and monocarboxylate transporter gene expression profiles in CNS metabolic sensing sites during acute and recurrent insulin-induced hypoglycemia", J. Mol. Neurosci, 31, pp.37~46 (2007)
- Wilson, M. C., "Basigin(CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4", J. Biol. Chem, 280, pp.27213~27221 (2005)
- Yuan, Q., "Age-dependent expression of the cystatin-related epididymal spermatogenic (Cres) gene in mouse testis and epididymis", Asian J. Androl, 9, pp.305~311 (2007)