Genetic Distances and Variations of Three Geographic Hairtail Populations Identified by PCR Analysis
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In the present study, muscle tissues were obtained separately from individuals from Atlantic hairtailpopulation (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The seven decamerprimers were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the threehairtail populations. Here, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population,55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number ofshared loci by the three populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28loci, with an average of 4 per primer, were observed in the CHP population. The hierarchical dendrogram point out three mainbranches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals’CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantlyrelated to CHINESE no. 19 (genetic distance = 0.430). Consequently, PCR analysis generated on the genetic data displayedthat the geographic AHP population was widely separated from CHP population, while individuals of CHP population werefairly closely related to those of GHP population.
Abstract
In the present study, muscle tissues were obtained separately from individuals from Atlantic hairtailpopulation (AHP), Gunsan hairtail population (GHP) and Chinese hairtail population (CHP), respectively. The seven decamerprimers were used to generate the shared loci, specific, unique shared loci to each population and shared loci by the threehairtail populations. Here, averagely, a decamer primer generated 64.7 amplified products per primer in the AHP population,55.7 in GHP population and 56.4 in CHP population. The number of unique shared loci to each population and number ofshared loci by the three populations generated by genetic analysis using 7 decamer primers in AHP, GHP and CHP population. 119 unique shared loci to each population, with an average of 17 per primer, were observed in the AHP population, and 28loci, with an average of 4 per primer, were observed in the CHP population. The hierarchical dendrogram point out three mainbranches: cluster 1 (ATLANTIC 01 ~ ATLANTIC 07), cluster 2 (GUNSAN 08 ~ GUNSAN 14) and cluster 3 (CHINESE 15~ CHINESE 21). The shortest genetic distance displaying significant molecular difference was between individuals’CHINESE no. 16 and CHINESE no. 18 (0.045). In the long run, individual no. 01 of the AHP population was most distantlyrelated to CHINESE no. 19 (genetic distance = 0.430). Consequently, PCR analysis generated on the genetic data displayedthat the geographic AHP population was widely separated from CHP population, while individuals of CHP population werefairly closely related to those of GHP population.
참고문헌 (14)
- Huang BX, "Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers", Mar Biol, 136, pp.207~216 (2000)
- M. S. Islam, "Genetic Analysis of Three River Populations of Catla catla (HAMILTON) Using Randomly Amplified Polymorphic DNA Markers", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 18(4), pp.453~457 (2005), 아세아·태평양축산학회
- Jeffreys AJ, "DNA fingerprints of dogs and cats", Anim Genet, 18, pp.1~15 (1987)
- Kim SH, "The study on the fisheries biological feature of hairtail, Trichiurus lepturus from the Cheju Strait", J Korean Fish Soc, 31(1), pp.17~25 (1998)
- 구자근, "범게 (Orithyia sinica Linnaeus) 유생의 생존, 성장 및 탈피주기에 미치는 수온의 영향", 한국어병학회지, 17(2), pp.139~144 (2004), 한국어병학회
- McCormack GC, "Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers", Mar Biotechnol, 2, pp.100~106 (2000)
- Muchmore ME, "Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone", Mol Mar Biol Biotechnol, 7, pp.1~6 (1998)
- Park CS, "Distribution and migration of hairtail, Trichiurus lepturus in Korean waters", J Korean Soc Fish Res, 5, pp.1~11 (2002)
- Su Young Park, "Genetic Differences and Variations in Slipper Lobster (Ibacus ciliatus) and Deep Sea Lobster (Puerulus sewelli) Determined by RAPD Analysis", Genes & Genomics, 27(4), pp.307~317 (2005), 한국유전학회
- Tassanakajon A, "Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis", J Mar Biotech, 6, pp.249~254 (1998)
- Yoke-Kqueen C, "Random amplified polymorphic DNA analysis of genetically modified organisms", J Biotechnol, 127, pp.161~166 (2006)
- Jong-ManYoon, "Genetic Differences between Cultured and Wild Penaeid Shrimp (Penaeus chinensis) Populations Analysed by RAPD-PCR", Genes & Genomics, 25(1), pp.4~32 (2003), 한국유전학회
- Jong-Man Yoon, "Genetic Differences within and between Populations of Korean Catfish (S. asotus) and Bullhead (P. fulvidraco) Analysed by RAPD-PCR", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(8), pp.1053~1061 (2004), 아세아·태평양축산학회
- Zhou L, "Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Block, based on detection of RAPD molecular markers", Cytogenet Cell Genet, 88, pp.133~139 (2000)