Genetic Distances and Variations of Three Clupeid Species Determined by PCR Technique
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In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, uniqueshared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean geneticdistances within- and between-species were also calculated by complete linkage method with the sustenance of thehierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Koreananchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified severaltimes by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individualPALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11(genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than didindividuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the geneticdata displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely,individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi)species.
Abstract
In this study, seven oligonucleotides primers were shown to generate the shared loci, specific loci, uniqueshared loci to each species and shared loci by the three species which could be obviously calculated. Euclidean geneticdistances within- and between-species were also calculated by complete linkage method with the sustenance of thehierarchical dendrogram program Systat version 13. The genomic DNA isolated from herring (Clupea pallasii), Koreananchovy (Coilia nasus) and large-eyed herring (Harengula zunashi), respectively, in the Yellow Sea, were amplified severaltimes by PCR reaction. The hierarchical dendrogram shows three chief branches: cluster 1 (PALLASII 01, 02, 03, 04, 06 and07), cluster 2 (NASUS 08, 09, 10, 11, 12, 13 and 14), and cluster 3 (ZUNASHI 15, 16, 17, 18, 19, 20, 21 and PALLASII 05). In three clupeid species, the shortest genetic distance displaying significant molecular difference was between individualPALLASII no. 03 and PALLASII no. 02 (0.018). Individual no. 06 of PALLASII was most distantly related to NASUS no. 11(genetic distance = 0.318). Individuals from herring (C. pallasii) species (0.920) exhibited higher bandsharing values than didindividuals from Korean anchovy (C. nasus) species (0.872) (P<0.05). As a result, this PCR analysis generated on the geneticdata displayed that the herring (C. pallasii) species was widely separated from Korean anchovy (C. nasus) species. Reversely,individuals of Korean anchovy (C. nasus) species were a little closely related to those of large-eyed herring (H. zunashi)species.
참고문헌 (14)
- 배재현, "생분해성 청어 (Clupea pallasii) 자망의 어획성능", 한국어업기술학회지, 49(4), pp.341~351 (2013), 한국어업기술학회[DOI]
- Diaz-Jaimes P, "Allozyme and RAPD variation in the eastern Pacific yellowfin tuna (Thunnus albacares)", Fish Bull, 101, pp.769~777 (2003)
- 허민수, "시판 청어(Clupea pallasii ) 과메기의 위생 및 영양학적 특성", 한국수산과학회지, 45(1), pp.1~10 (2012), 한국수산과학회
- M. S. Islam, "Genetic Analysis of Three River Populations of Catla catla (HAMILTON) Using Randomly Amplified Polymorphic DNA Markers", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 18(4), pp.453~457 (2005), 아세아·태평양축산학회
- Jeffreys AJ, "DNA fingerprints of dogs and cats", Anim Genet, 18, pp.1~15 (1987)
- Kim JK, "A new record of the herring, Sardinella lemuru (Pisces: Clupeidae) from Korea", Korean J Ichthyol, 13, pp.190~194 (2001)
- 김종래, "삼척과 원산의 지리적 민들조개(Gomphina aequilatera, Sowerby) 집단의 유전적 변이", 발생과 생식, 10(4), pp.227~238 (2006), 한국발생생물학회
- Mamuris Z, "Taxonomic relationships between four species of the Mullidae family revealed by three genetic methods:allozymes, random amplified polymorphic DNA and mitochondrial DNA", J Fish Biol, 55, pp.572~587 (1999)
- McCormack GC, "Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers", Mar Biotechnol, 2, pp.100~106 (2000)
- Su Young Park, "Genetic Differences and Variations in Slipper Lobster (Ibacus ciliatus) and Deep Sea Lobster (Puerulus sewelli) Determined by RAPD Analysis", Genes & Genomics, 27(4), pp.307~317 (2005), 한국유전학회
- Partis L, "Identification of fish species using random amplified polymorphic DNA (RAPD)", Mol Cell Probes, 10, pp.435~441 (1996)
- Young-Jae Song, "Genetic Differences of Three Pollicipes mitella Populations Identified by PCR Analysis", 발생과 생식, 17(3), pp.199~205 (2013), 한국발생생물학회
- Yoke-Kqueen C, "Random amplified polymorphic DNA analysis of genetically modified organisms", J Biotechnol, 127, pp.161~166 (2006)
- Jong-Man Yoon, "Genetic Differences within and between Populations of Korean Catfish (S. asotus) and Bullhead (P. fulvidraco) Analysed by RAPD-PCR", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 17(8), pp.1053~1061 (2004), 아세아·태평양축산학회