cyclic GMP Mediated Inhibition of Spontaneous Germinal Vesicle Breakdown Both with and without Cumulus in Mouse Oocyte
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Intact germinal vesicle (GV) arrest and release are essential for maintaining the fertility of mammals inducing human. Intact germinal vesicle release, maturation of oocytes is maintained by very complex procedures along with folliculogenesis and is a critical step for embryonic development. Cyclic guanosine monophosphate (cGMP) has been suggested a key factor for meiotic arrest but so far its mechanisms are controversy. In this study we examine the effects of cGMP on germinal vesicle breakdown in cumulus-enclosed oocytes and denuded oocytes. Spontaneous maturation was inhibited by a cGMP agonist, 8-Br-cGMP with concentration dependent manners both in cumulus-enclosed oocytes and denuded oocytes. The inhibitory effect was more severe in denuded oocytes than cumulus-enclosed oocytes. The Rp-8-Br-cGMP and Rp-pCPT- 8-Br-cGMP did not severely block GVB compared to 8-Br-cGMP. The spontaneous GVB inhibitory effects were different by the existence of cumulus. Based on them it is suggested that the cumulus modulates the role of cGMP in GV arrest.
Abstract
Intact germinal vesicle (GV) arrest and release are essential for maintaining the fertility of mammals inducing human. Intact germinal vesicle release, maturation of oocytes is maintained by very complex procedures along with folliculogenesis and is a critical step for embryonic development. Cyclic guanosine monophosphate (cGMP) has been suggested a key factor for meiotic arrest but so far its mechanisms are controversy. In this study we examine the effects of cGMP on germinal vesicle breakdown in cumulus-enclosed oocytes and denuded oocytes. Spontaneous maturation was inhibited by a cGMP agonist, 8-Br-cGMP with concentration dependent manners both in cumulus-enclosed oocytes and denuded oocytes. The inhibitory effect was more severe in denuded oocytes than cumulus-enclosed oocytes. The Rp-8-Br-cGMP and Rp-pCPT- 8-Br-cGMP did not severely block GVB compared to 8-Br-cGMP. The spontaneous GVB inhibitory effects were different by the existence of cumulus. Based on them it is suggested that the cumulus modulates the role of cGMP in GV arrest.
참고문헌 (20)
- Y. P. Cheon, "Guanosine Regulates Germinal Vesicle Breakdown (GVBD) in Mouse Oocytes", Reproductive & Developmental biology, 28(4), pp.267~273 (2004), 한국동물번식학회
- Dutta S, "Arrst at the diplotene stage of meiotic prophase I is delayed by grogesterone but is not required for primodial follicle formation in mice", Reprod Biol Endocrinol, 14, pp.82~ (2016)
- Egbert JR, "Dephosphorylation and inactivation of NPR2 guanylyl cyclase in granulosa cells contributes to the LH-induced decrease in cGMP that causes resumption of meiosis in rat oocytes", Development, 141, pp.3594~3604 (2014)
- Eppig JJ, "Hypoxanthine and adenosine in murine ovarian follicular fluid: concentrations and activity in maintaining oocyte meiotic arrest", Biol Reprod, 33, pp.1041~1049 (1985)
- Erickson GF, "In vitro matruation of mouse oocytes isolated from late, middle, and preantral Graafian follicles", J Exp Zool, 190, pp.123~127 (1974)
- Hambleton R, "Isoforms of cyclic nucleotide phosphodiesterase PDE3 and their contribution to cAMP hydrolytic activity in subcellular fractions of human myocardium", J Biol Chem, 280, pp.39168~39174 (2005)
- Hubbard CJ, "Ovarian cAMP and cGMP flucturations in the hamster during the oestrous cycle", J Reprod Fertil, 59, pp.351~355 (1980)
- Hubbard CJ, "Inhibitory action of cyclic guanosine 5’-phosphoric acid (GMP) on oocyte maturation: dependence on an intact cumulus", Biol Reprod, 26, pp.628~632 (1982)
- 황희경, "Adenosine Receptors Mediated Intracellular Calcium in Cumulus Cells Involved in the Maintenance of First Meiotic Arrest", 발생과 생식, 17(2), pp.141~147 (2013), 한국발생생물학회
- Jankowski M, "C-type natriuretic peptide and the guanylyl cyclase receptors in the rat ovary are modulated by the estrous cycle", Biol Reprod, 56, pp.59~66 (1997)
- Jones KT, "Meiosis in oocytes: predisposition to aneuploidy and its increased incidence with age", Hum Reprod update, 14, pp.143~158 (2008)
- Mehlmann LM, "Oocyte-specific expression of Gpr3 is required for the maintenance of meiotic arrest in mouse oocytes", Dev Biol, 288, pp.397~404 (2005)
- Norris RP, "Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte", Development, 136, pp.1869~1878 (2009)
- Pincus G, "The comparative behavior of mammalian eggs in vivo and in vitro", J Exp Med, 62, pp.665~675 (1935)
- Powers RD, "Combined effects of calcium and dibutyryl cyclic AMP on germinal vesicle breakdown in the mouse oocyte", J Reprod Fertil, 66, pp.1~8 (1982)
- Preston SL, "Adenosine reverses calcium-dependent inhibition of follicle-stimulating hormone action and induction of maturation in cumulusenclosed rat oocytes", Endocrinology, 120, pp.1346~1353 (1987)
- Robinson JW, "Luteinizing hormone reduces the activity of the NPR2 guanylyl cyclase in mouse ovarian follicles, contributing to the cyclic GMP decrease that promotes resumption of meiosis in oocytes", Dev Biol, 366, pp.308~316 (2012)
- Shuhaibar LC, "Intracellular signaling via cyclic GMP diffusion through gap junctions restarts meiosis in mouse ovarian follicles", Proc Natl Acad Sci U S A, 112, pp.5527~5532 (2015)
- Vaccari S, "Cyclic GMP signalings is involved in the luteinizing hormone-dependent meiotic maturation of mouse oocytes", Biol Reprod, 81, pp.595~604 (2009)
- Zhang M, "Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes", Science, 330, pp.366~369 (2010)