Adenosine Receptors Mediated Intracellular Calcium in Cumulus Cells Involved in the Maintenance of First Meiotic Arrest
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Keeping the intact germinal vesicle (GV) is essential for maintaining the capacity of mammals including human. It is maintained by very complex procedures along with folliculogenesis and is a critical step for getting competent oocyte. So far, a few mechanisms involved in folliculogenesis are known but GV arrest mechanisms are largely unrevealed. Cyclic AMP, a adenosine derived substance, have been used as inhibitor of germinal vesicle breakdown as a putative oocyte maturation inhibitor. In this study, we examined the potency of adenosine as GV maintainer and a possible signaling mediator for that. A1, A2b, and A3 were detected in cumulus cells of cumulus enclosed-oocyte (CEO). Intact of germinal vesicle was not kept like in follicle but the spontaneous maturation was inhibited by exogenous adenosine. It is inhibited with concentration dependent manners. Intracellular calcium level of cumulus was extensively increased after adenosine treatment. Based on these results it is suggested that one of the pathway for GV arrest by adenosine and its receptors is calcium mediated signaling pathway in CEO.
Abstract
Keeping the intact germinal vesicle (GV) is essential for maintaining the capacity of mammals including human. It is maintained by very complex procedures along with folliculogenesis and is a critical step for getting competent oocyte. So far, a few mechanisms involved in folliculogenesis are known but GV arrest mechanisms are largely unrevealed. Cyclic AMP, a adenosine derived substance, have been used as inhibitor of germinal vesicle breakdown as a putative oocyte maturation inhibitor. In this study, we examined the potency of adenosine as GV maintainer and a possible signaling mediator for that. A1, A2b, and A3 were detected in cumulus cells of cumulus enclosed-oocyte (CEO). Intact of germinal vesicle was not kept like in follicle but the spontaneous maturation was inhibited by exogenous adenosine. It is inhibited with concentration dependent manners. Intracellular calcium level of cumulus was extensively increased after adenosine treatment. Based on these results it is suggested that one of the pathway for GV arrest by adenosine and its receptors is calcium mediated signaling pathway in CEO.
참고문헌 (23)
- Carroll AG, "Inhibition of germinal vesicle breakdown and activation of cytoplasmic contractility in Spisula oocytes by calmodulin antagonist", Biol Bull, 170, pp.43~50 (1986)
- Downs SM, "Uptake and metabolism of adenosine mediate a meiosis-arresting action on mouse oocytes", Mol Reprod Dev, 53, pp.208~221 (1999)
- Downs SM, "Induction of meiotic maturation in mouse oocytes by adenosine analogs", Mol Reprod Dev, 73, pp.1159~1168 (2006)
- Downs SM, "Hypoxanthine is the inhibitor of murine oocyte maturation in a low molecular weight fraction of porcine follicular fluid", Proc Natl Acad Sci USA, 82, pp.454~458 (1985)
- Ekholm C, "Stimulation and inhibition of rat oocyte meiosis by forskolin", Biol Reprod, 30, pp.537~543 (1984)
- Epperson SA, "Adenosine receptors and second messenger signaling pathways in rat cardiac fibroblasts", Am J Physiol Cell Physiol, 296, pp.C1171~C1177 (2009)
- Eppig JJ, "Inhibition of oocyte maturation in the mouse: participation of cyclic AMP, steroids, and a putative maturation inhibitory factor", Dev Biol, 100, pp.39~49 (1983)
- Eppig JJ, "Hypoxanthine and adenosine in murine ovarian follicular fluid: concentrations and activity in maintaining oocyte meiotic arrest", Biol Reprod, 33, pp.1041~1049 (1985)
- Kobayashi T, "Functional characterization of an endogenous Xenopus oocyte adenosine receptor", Br J Pharmacol, 135, pp.313~322 (2002)
- Kotani S, "PKA MPFactivated Polo-like kinase regulate anatphase-promoting complex activity and mitosis progression", Mol Cell, 1, pp.371~380 (1998)
- Machaca K, "Induction of maturationpromoting factor during Xenopus oocyte maturation uncouples Ca2+ store depletion from store-operated Ca2+ entry", J Cell Biol, 156, pp.75~86 (2002)
- Marangos P, "Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1)", J Cell Biol, 176, pp.65~75 (2007)
- Miller JG, "Oocyte maturation is inhibited by adenosine in the presence of folliclestimulating hormone", Biol Reprod, 35, pp.833~837 (1986)
- Newhall KJ, "Dynamic anchoring of PKA is essential during oocyte maturation", Curr Biol, 16, pp.321~327 (2006)
- Paleos GA, "The effect of calcium on the first meiotic division of the mammalian oocyte", J Exp Zool, 217, pp.409~416 (1981)
- Powers RD, "Combined effects of calcium and dibutyryl cyclic AMP on germinal vesicle breakdown in the mouse oocyte", J Reprod Fertil, 66, pp.1~8 (1982)
- Preston SL, "Adenosine reverses calcium-dependent inhibition of follicle-stimulating hormone action and induction of maturation in cumulusenclosed rat oocytes", Endocrinology, 120, pp.1346~1353 (1987)
- Reis A, "APcdh1 activity in mouse oocytes prevents entry into the first meiotic division", Nat Cell Biol, 8, pp.539~540 (2006)
- Salustri A, "Adenosine potentiates forskolin-induced delay of meiotic resumption by mouse denuded oocytes: evidence for an oocyte surface site of adenosine action", Gamete Res, 21, pp.157~168 (1988)
- Sun L, "Kinase-dependent regulation of inositol 1,4,5- triphosphate-dependent Ca2+ release during oocyte maturation", J Biol Chem, 284, pp.20184~20196 (2009)
- Tsafriri A, "In vitro induction of meiotic division in follicle enclosed rat oocytes by LH, cyclic AMP, and prostaglandin E2", J Reprod Fertil, 31, pp.39~50 (1972)
- Xia Y, "Distinct KATP channels mediate the antihypertrophic effects of adenosine receptor activation in neonatal rat ventricular myocytes", J Pharmacol Exp Ther, 320, pp.14~21 (2007)
- Zhang M, "Hormonal control of mammalian oocyte meiosis at diplotene stage", Cell Mol Life Sci, 69, pp.1279~1288 (2012)