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Ethane 1,2-Dimethane Sulfonate (EDS)가 성체 흰쥐의 부속생식기관에 미치는 효과 : 조직학적 연구

Effect of ethane 1,2-dimethane sulfonate(EDS) on the accessory sex organs in adult rats : a histological study

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한국발생생물학회 (The Korea Society Of Developmental Biology)|2009.06|발생과 생식 (DEVELOPMENT & REPRODUCTION)|제13권 제2호|pp. 105-114

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참고문헌

주제분류생물학

초록

알킬화제로 잘 알려진 ethane 1,2-dimethane sulfonate(EDS)는 여러 종에서 선택적인 Leydig 세포(LC) 독성과 정소 기능장애에 연구모델로 널리 사용된다. EDS 투여에 의해 유도된 LC 녹아웃 흰쥐의 경우 부정소와 저정낭과 같은 테스토스테론 의존성 부속생식기관들의 급격한 무게 감소가 초래됨이 이전의 연구들에서 보고되었다. 본 연구는 EDS 투여가 흰쥐 부속생식기관에 미치는 영향에 대해 조사한 것이다. 성체 수컷 흰쥐에 EDS를 1회 주사(75mg/kg, i.p.) 한 후 0, 1, 2, 3, 4, 5, 6, 7주 후에 각각 희생시켰다. 정소와 부정소, 저정낭 그리고 전립선의 무게를 각각 측정하였다. 조직의 변화는 hematoxylin & eosin 염색과 광학현미경을 통하여 분석하였다. 생식소와 그 부속기관의 무게는 EDS 처리 후 3주까지 점진적으로 감소하였고, 이후 서서히 정상으로 회복되었다. 부정소의 무게는 정상 수준의 60%까지 회복되는 양상을 보였다. 조직학적 결과에서 부정소의 상피세포층은 주사 후 1주에서 3주까지 비대해지는 것이 관찰되었다. 저정낭에서는 주사후 1주부터 3주까지 상피세포층의 두께가 감소하였다. 전립선 역시 주사후 1주에서 3주까지 상피세포층의 두께가 감소되고 4주부터 회복되었다. 본 연구는 EDS 주사에 의해 일시적으로 안드로겐 결핍이 일어난 흰쥐에서 부속생식기관에 뚜렷한 조직학적 변화가 일어남을 보였다. EDS 모델을 사용하여 이들 안드로겐 의존적인 부속생식기관의 생리적, 분자적인 조절 기작에 대해 심층 연구할 경우 이들 조직의 정상 및 병리학적 발생과 분화를 이해하는데 도움이 될 것이다.

Abstract

Ethane 1,2-dimethane sulfonate(EDS) is a well-known alkylating agent used as selective Leydig cell(LC) toxicant to create a testicular dysfunction model. Previous studies including our own clearly demonstrated the dramatic weight loss of the androgen dependent accessory sex organs such as epididymis, seminal vesicle and prostate gland in this 'LC knock-out' rats. The present study was performed to evaluate the effect of EDS administration on histological changes of the epididymis, seminal vesicle and prostate in adult rats. Adult male Sprague-Dawley rats(350～400g B.W.) were injected with a single dose of EDS(75mg/kg, i.p.) and sacrificed on weeks 0, 1, 2, 3, 4, 5, 6 and 7. Tissue weights(testis, epididymis, seminal vesicle and prostate gland) were measured. The histological changes of tissue were observed by a light microscopy using hematoxylin & eosin staining. Weights of the reproductive and accessory organs progressively declined after the EDS treatments(weeks 1, 2 and 3). After this, the decrease was stopped, then gradually returned to the norma levels. There was a partial(about 60%) recovery of the epididymis weight during weeks 6-7. The cross section of epididymis revealed an increase in thickness of the epithelium during weeks 1-3. In contrast, considerable reduction of epithelial thickness in seminal vesicle was observed during same period. Similarly, a reduction in thickness of prostate epithelial layer was found during weeks 1-3, then it was back to normal thickness after week 4. Taken together, the present study demonstrated that the temporally induced androgen-deficiency by EDS treatment could result the prominent alterations in histology of the accessory sex organs. Further studies on the physiological and molecular regulation of these androgen-sensitive organs using EDS model will be helpful to understand the normal and pathological development and differentiation mechanism of these organs.

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관련 논문AI추천

수컷 흰쥐 뇌하수체의 생식소자극호르몬 발현에 미치는 Ethane 1,2-Dimethane Sulfonate(EDS)의 효과김수웅, 백재승, 손혁준, 이성호한국발생생물학회 · 발생과 생식2007 · 제11권 제1호흰쥐 부정소에서의 세포자연사에 미치는 Ethan 1,2-Dimethane Sulfonate(EDS)의 효과손혁준, 이성호한국발생생물학회 · 발생과 생식2006 · 제10권 제3호흰쥐 부정소 내의 스테로이드 호르몬 수용체, 5α-reductase 그리고 aromatase 발현에 미치는 EDS의 영향손혁준, 이성호한국발생생물학회 · 발생과 생식2007 · 제11권 제3호흰쥐에서 한쪽 고환 거세 후 고환간질세포의 형태계측학적 연구강형섭, 김인식, 나성진, 박상열, 안동춘, 양홍현, 이기창, 이석원, 이영덕, 태현진대한체질인류학회 · 해부·생물인류학2008 · 제21권 제4호고장성 식염수 주사에 의한 흰쥐 정소의 해부학적 변화곽병국, 이성호, 이철상한국발생생물학회 · 발생과 생식2010 · 제14권 제4호수컷 생쥐 부속 생식샘의 형태에 미치는 에스트로겐 수용체 α의 선택적 효능제 propyl pyrazole triol의 영향조현욱, 한지연한국현미경학회 · Applied Microscopy2009 · 제39권 제1호

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