흰쥐에서 한쪽 고환 거세 후 고환간질세포의 형태계측학적 연구
Morphometric Studies in the Leydig Cell of Hemicastrated Rats
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수컷 흰쥐에서 각기 다른 일령에 한쪽 고환을 제거하고 일정한 시간경과 후 남아 있는 고환 간질세포의 형태계측학적인 변화를 살펴보기 위하여 본 연구를 수행하였다. 출생 후 20, 30, 40, 50 및 60일령(n=18마리/일령)의 흰쥐 한쪽 고환을 거세한 후 100일령에 희생시켜 실험에 이용하였다. 흰쥐를 전신관류고정하고 고환을 적출 한 후 조직 처리과정을 거쳐 초박절편한 다음 methylene-blue 염색을 하여 일반적인 조직학적인 변화를 관찰하고 형태계측을 시행하였다. 황체형성호르몬 자극에 대한 고환내 테스토스테론 생성과 혈청내 테스토스테론 농도는 방사면역측정법을 이용하여 측정하였다. 실험군과 대조군의 몸무게는 차이가 없었지만 대조군이 실험군보다 그 값이 높았으며 고환 무게도 유의성 있 는 차이는 없었으나 30, 40 및 60일령에 거세한 실험군에서 대조군에 비해 약간 높았다. 곱슬정세관, 간질조직 및 간질세포의 용적치밀도는 영향이 없었으나 절대용적은 20, 30 및 40일령에 거세한 실험군에서 유의성 있게 증가 하였다 (P⁄0.05). 고환조직 당 간질세포의 총 숫자는 60일령을 제외한 전 실험군에서 유의성 있게 증가하였다 (P⁄0.05). 간질세포의 평균용적, 혈청내 테스토스테론농도 및 황체형성호르몬자극에 대한 고환내 테스토스테론 분비는 30일과 40일령에 거세한 실험군에서 대조군에 비하여 유의성 있게 증가하였다(P⁄0.05). 이상을 종합하면 흰쥐에서 30일령과 40일령에 한쪽 고환을 거세하면 성 성숙후에 남아 있는 고환의 보상작용 으로 간질세포의 크기와 분비가 증가함을 확인하였고 흰쥐에서 성 성숙이 이루어지는 즈음에 거세를 하면 간질세 포의 보상작용이 이루어지는 것을 알 수 있었다.
Abstract
The present study was conducted to investigate the influence of hemicastration and age at hemicastraion on the subsequent Leydig cell morphology and function of male rats. Sprague Dawley rats were left intact or hemicastrated at 20, 30, 40, 50, or 60 days of age (n=18 rats per group). At 100 days of age, all rats were sacrificed. Testes were fixed by whole body perfusion using a fixative containing 2.5% glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 μm sections stained with methylene blue, qualitative and quantitative morphological studies were performed. Testis incubations were used to determine lutenizing hormone (LH; 100 ng/mL) stimulated testosterone secretory capacity per testis in vitro. Testosterone levels in the incubation medium, and testosterone and LH levels in serum of these six groups of rats were determined by radioimmunoassay. Body and testis weights were not changed by hemicastration between experimental and control groups. Volume density of seminiferous tubules, interstitium, and Leydig cells was not significantly affected by hemicastration. Absolute volume of seminiferous and interstitium was significantly increased in unilaterally castrated rats at 20, 30 and 40 days of age compared to control. Significant increases in the total number of Leydig cells per testis occurred in rats hemicastrated at 20, 30, 40 and 50 days of age compared to control. A significant increase in average volume of a Leydig cell was noted in the hemicastrated rats at 30 and 40 days compared to intact rats of the same age but was significantly decreased at 60 days of age. Serum testosterone levels and LH-stimulated testosterone production per testis were significantly (P⁄0.05) increased in the hemicastrated rats at 30 and 40 days. In summary, when rats were unilaterally castrated at 20, 30, 40, 50, and 60 days of age, those rats hemicastrated at 30 and 40 days showed compensatory hypertrophy/hypersecretion of Leydig cells when killed at 100 days of age. Especially, these data suggested that compensatory hypertrophy/hypersecretion of Leydig cells in rats hemicastrated around the time of puberty occurs in the remaining testis.
참고문헌 (33)
- Ariyaratne HB, "Changes in the testis interstitium of Sprague Dawley rats from birth to sexual maturity", Biol Reprod, 62, pp.680~690 (2000)
- Boockfor FR, "Effects of unilateral castration and unilateral cryptorchidism of the holstein bull on plasma gonadotropin, testosterone and testis anatomy", J Anim Sci, 56, pp.1376~1385 (1983)
- Bortolussi M, "Sertoli and Leydig cell numbers and gonadotropin receptors in rat testis from birth to puberty", Cell Tissue Res, 260, pp.185~191 (1990)
- Cunningham GR, "Mechanisms for the testicular hypertrophy which follows hemicastration", Endocrinology, 102, pp.16~23 (1978)
- Ewing, LL, "Leydig cell structure and steroidogenic function", Recent Prog Horm Res, 39, pp.599~635 (1983)
- Fawke L, "Effects of hemicastration and the subsequent administration of drugs in the mouse", J Reprod Fertil, 28, pp.177~183 (1972)
- Furuya T, "Onset of compensatory hypertrophy of interstitial tissue and Leydig cells in rats hemicastrated around the time of puberty", Biol Reprod, 42, pp.491~498 (1990)
- Howard HJ, "Relationships among concentrations of steroids, inhibin, insulin-like growth factor-l (IGF-1), and IGF-binding proteins during follicular development in weaned sows", Biol Reprod, 47, pp.193~201 (1992)
- Ichihara I, "Ultrastructure and morphometry of testicular Leydig cells and the interstitial components correlated with testosterone in aging rats", Cell Tissue Res, 27, pp.241~255 (1993)
- Kerr JB, "Alterations of the fine structure and androgen secretion of the interstitial cells in the experimentally cryptorchid rat testis", Biol Reprod, 20, pp.409~422 (1979)
- Kim IS, "Effects of continuous and intermittent exposure of lactating mother to Aroclor 1242 on testicular steroidogenic function in the adult male offspring", Tissue Cell, 33, pp.169~177 (2001)
- Kim IS, "Changes in the testis interstitium of Brown Norway rats with aging and effects of luteinizing and thyroid hormones on the aged testes in enhancing the steroidogenic potential", Biol Reprod, 66, pp.1359~1366 (2002)
- Kittok RJ, "Effect of castration on plasma luteinizing hormone concentrations in prepubertal boars", J Anim Sci, 58, pp.1271~1277 (1984)
- Kosco MS, "Endocrine responses in relation to compensatory testicular growth after neonatal hemicastration in boars", Biol Reprod, 36, pp.1177~1185 (1987)
- Kosco MS, "Development of the seminiferous tubules after neonatal hemicastration in the boar", J Reprod Fertil, 87, pp.1~11 (1989)
- Land RB, "Testis growth and plasma LH concentration following hemicastration and its relation with female prolificacy in sheep", J Reprod Fertil, 45, pp.495~501 (1975)
- Lee MH, "Studies on reappearance of thymus after orchidectomy in ageing rats.", Korean J Vet Res, 29, pp.223~229 (1989)
- Liang DS, "Testicular hypertrophy in rats", J Reprod Fertil, 21, pp.537~540 (1970)
- Lipsett MB, "Testosterone synthesis by the fetal rabbit gonad", Endocrinology, 77, pp.273~277 (1965)
- Lunstra DD, "Sertoli cells in the boar testis: changes during development and compensatory hypertrophy after hemicastration at different ages", Biol Reprod, 68, pp.140~150 (2003)
- Mendis-Handagama SM, "Signs of aging are apparent in the testis interstitium of Sprague Dawley rats at 6 months age", Tissue Cell, 27, pp.689~699 (1995)
- Mendis-Handagama SM, "Morphometric analysis of the components of the neonatal and adult rat testis interstitium", Int J Androl, 10, pp.525~534 (1987)
- Mendis-Handagama SM, "Comparison of components of the testis interstitium with testosterone secretion hamster, rat, and guinea pig testes perfused in vitro", Am J Anat, 188, pp.12~22 (1988)
- Mirando MA, "The influence of unilateral castration on testicular morphology and function in adult rams", Biol Reprod, 41, pp.798~806 (1989)
- Moger WH, "Endocrine responses of the prepubertal male rat to hemiorchidectomy", Biol Reprod, 17, pp.661~667 (1977)
- Ojeda SR, "Plasma level of LH and FSH in maturing rats: response to hemigonadectomy", Endocrinology, 90, pp.466~472 (1972)
- Ott KM, "Effects of unilateral castration on the physiological and morphological characteristics of stallion testes", J Anim Sci, 59, pp.355~356 (1984)
- Agee J, "Morphologic response of rat Leydig cells to hemicastration", Arch Androl, 20, pp.1~9 (1988)
- Russell LD, "Building a testis", Tissue Cell, 27, pp.129~147 (1995)
- Saez JM, "Leydig cell : endocrine, paracrine, and autocrine regulation", Endrocrine Rev, 15, pp.574~626 (1994)
- Umapathy E, "Effect of castration on serum concentrations of gonadal hormones, insulin-like growth factor-I and its binding proteins in male pigs", Reprod Nutr Dev, 37, pp.541~549 (1997)
- Voglmayr JK, "Compensatory hypertrophy in the remaining testis following unilateral orchidectomy in the adult ram", J Reprod Fertil, 17, pp.179~181 (1968)
- Wilson JD, "Developmental pattern of testosterone synthesis in the fetal gonad of the rabbit", Endocrinology, 92, pp.1182~1191 (1973)