수컷 생쥐 부속 생식샘의 형태에 미치는 에스트로겐 수용체 α의 선택적 효능제 propyl pyrazole triol의 영향
Effects of Estrogen Receptor α-Selective Agonist Propyl Pyrazole Triol on the Morphology of Accessory Genital Glands of Male Mouse
2
조회
0
다운로드
4
인용
0
참고문헌
초록
본 연구에서는 에스트로겐 수용체 알파 효능제인 propyl pyrazole triol (PPT)을 수컷 생쥐에 투여하였을 때 부속 생식샘의 조직학적 변화가 유발되는지를 조사하였다. 광학현미경으로 조사하기 위해 수컷 생식기관을 고정, 탈수, 포매, 절편 과정을 거쳐 프레파라트를 완성하였다. PPT에 의해 복부쪽 전립샘, 정낭, 포피샘의 각 무게는 실험기간에 따라 감소되었다. 투여군의 경우, 복부쪽 전립샘의 샘 조직 내강은 위축되었다. 전립샘 상피조직의 형태가 단층 원주상피에서 중층 입방 상피 혹은 편평상피로 변화되었다. 투여군 전립샘과 정낭의 상피조직 아래에 있는 결합조직은 증가되었다. 특히, 투여군 정낭의 내강이 대조군에 비해 위축되었다. 포피샘의 투여군 8주에서 상피세포 높이가 감소되었 다. 수컷 내에서 에스트로겐 수용체 효능제의 이런 영향을 파악함으로서 생식기관 내 에스트로겐의 생리학적 기능을 이해하는데에 도움을 줄 것으로 사료된다.
Abstract
This study investigated that exposure of male mice to estrogen receptor α-selective agonist, 4,4′,4′′-(4-propyl-[1H]-pyrazole- 1,3,5-triyl)tris-phenol (PPT) induce morphological changes of accessory genital glands. The male reproductive organs were fixed and processed for light microscopy. The PPT induced decreases of ventral prostates, seminal vesicles and preputial glands weights with experimental time. The glandular lumen of ventral prostate was atrophied compared with control group. Type of epithelial tissues in the prostate was changed from simple columnar epithelium to stratified cuboidal or squamous epithelium. Treated group with the agonist showed that increased connective tissue underlying epithelium in the prostate and seminal vesicle. Especially, the glandular lumen of the seminal vesicle was contracted when PPT-treated animals were compared with control group. Secretion cells of preputial gland were smaller than that of control group. On week 8, PPT treatment caused decrease of epithelial cell height lining the lumen of preputial gland. These results provide information useful in researching the physiological function of estrogen mediated by estrogen receptor α in male accessory genital gland.
참고문헌 (38)
- Ago A, "Morphological studies on preputial gland of mice. 1. Light and transmission electron microscopic observations of preputial gland in male mice", Exp Anim, 43, pp.645~649 (1995)
- Aleem M, "Estradiol affects androgen-binding protein expression and fertilizing ability of spermatozoa in adult male rats", Mol Cell Endocrinol, 253, pp.1~13 (2006)
- Atanassova N, "Permanent effects of neonatal estrogen exposure in rats on reproductive hormone levels, Sertoli cell number, and the efficiency of spermatogenesis in adulthood", Endocrinol, 140, pp.5364~5373 (1999)
- Cho HW, "The antiestrogen ICI 182,780 induces early effects on the adult male mouse reproductive tract and long-term decreased fertility without testicular atrophy", Reprod Biol Endocrinol, 1(57) (2003)
- Couse JF, "Estrogen receptor null mice: What have we learned and where will they lead us?", Endocrine Rev, 20, pp.358~417 (1999)
- Couse JF, "Estrogen receptor-α mediates the detrimental effects of neonatal diethylstilbestrol (DES) exposure in the murine reproductive tract", Toxicol, 205, pp.55~63 (2004)
- Couse JF, "Tissue distribution and quantitative analysis of estrogen receptor-α (ERα) and estrogen receptor-β (ERβ) messenger ribonucleic acid in the wild-type and ERα-knockout mouse", Endocrinol, 138, pp.4613~4621 (1997)
- Cunha GR, "Estrogenic effects on prostatic differentiation and carcinogenesis", Reprod Fertil Dev, 13, pp.285~296 (2001)
- Gonzales GF, "Red maca (Lepidium meyenii) reduced prostate size in rats", Reprod Biol Endocrinol, 3(5) (2005)
- Goyal HO, "Diethylstilbestrol-treated adult rats with altered epididymal sperm numbers and sperm motility parameters, but without alterations in sperm production and sperm morphology", Biol Reprod, 64, pp.927~934 (2001)
- Goyal HO, "Exposure of neonatal male rats to estrogen induces abnormal morphology of the penis and loss of fertility", Reprod Toxicol, 18, pp.265~274 (2004)
- Goyal HO, "Neonatal estrogen exposure of male rats alters reproductive functions at adulthood", Biol Reprod, 68, pp.2081~2091 (2003)
- Han HJ, "Response of primary rabbit kidney proximal tubule cells to estrogens", J Cell Physiol, 178, pp.35~43 (1999)
- Hess RA, "Estrogen in the adult male reproductive tract", Reprod Biol Endocrinol, 1, pp.52~ (2003)
- Hess RA, "A role for oestrogens in the male reproductive system", Nature, 390, pp.509~512 (1997)
- Imamov O, "Estrogen receptor β regulates epithelial cellular differentiation in the mouse ventral prostate", PNAS, 101, pp.9375~9380 (2004)
- Jarred RA, "Evidence that estrogens directly alter androgen-regulated prostate development", Endocrinol, 141, pp.3471~3477 (2000)
- Jarred RA, "Prostate phenotypes in estrogen-modulated transgenic mice", Trends in Endocrinol & Metabolism, 13, pp.163~168 (2002)
- Kaneto M, "Epidermal sperm motion as a parameter of male reproductive toxicity: sperm motion, fertility, and histopathology in ethinylestradiol-treated rats", Reprod Toxicol, 13, pp.279~289 (1999)
- Kilpeläinen PT, "Abnormal ornithine decarboxylase activity in transgenic mice increases tumor formation and infertility", IJBCB, 33, pp.507~520 (2001)
- Kuiper GGJM, "Cloning of a novel estrogen receptor expressed in rat prostate and ovary", PNAS, 93, pp.5925~5930 (1996)
- Lau KM, "Rat estrogen receptor-α and -β, and progesterone receptor mRNA expression in various prostatic lobes and microdissected normal and dysplastic epithelial tissues of the Noble rats", Endocrinol, 139, pp.424~427 (1998)
- LaValle TM, "2-methoxyestradiol inhibits proliferation and induces apoptosis independently of estrogen receptors α and β", Cancer Res, 62, pp.3691~3697 (2002)
- Mäkelä S, "Differential expression of estrogen receptors α and β in adult rat accessory sex glands and lower urinary tract", Mol Cell Endocrinol, 164, pp.109~116 (2000)
- Nilsson S, "Mechanism of estrogen action", Physiol Rev, 81, pp.1535~1565 (2001)
- O’Donnell L, "Estrogen and spermatogenesis", Endocrine Rev, 22, pp.289~318 (2001)
- Oliveira CA, "The antiestrogen ICI 182,780 decreases the expression of estrogen receptor-alpha but has no effect on estrogen receptor-beta and androgen receptor in rat efferent ductules", Rep-rod Biol Endocrinol, 1, pp.75~ (2003)
- Pelletier G, "Localization of oestrogen receptor α, oestrogen receptor β and androgen receptors in the rat reproductive organs", J Endocrinol, 165, pp.359~370 (2000)
- Prins GS, "Estrogen imprinting of the developing prostate gland is mediated through stromal estrogen receptor α: Studies with αERKO and βERKO mice", Cancer Res, 61, pp.6089~6097 (2001)
- Risbridger GP, "The metaplastic effects of estrogen on mouse prostate epithelium: Proliferation of cells with basal cell phenotype", Endocrinol, 142, pp.2443~2450 (2001)
- Saunders PT, "Expression of oestrogen receptor beta (ER beta) in multiple rat tissues visualized by immunohistochemistry", J Endocrinol, 154, pp.R13~16 (1997)
- Stauffer SR, "Pyrazole ligands: Structure-affinity", activity relationships and estrogen receptor-α-selective agonists, 2000, pp.43, 4934~4947
- Sun J, "Antagonists selective for estrogen receptor α", Endocrinol, 143, pp.941~947 (2002)
- Taylor RA, "17β- estradiol induces apoptosis in the developing rodent prostate independently of ERα or ERβ", Endocrinol, 147, pp.191~200 (2006)
- Toft D, "A receptor molecule for estrogens: Isolation from the rat uterus and preliminary characterization", PNAS, 55, pp.1574~1581 (1966)
- Tsuji M, "Later onset of apoptosis in the bulbourethral glands after castration compared to that in the seminal vesicles", J Steroid Biochem Mol Biol, 67, pp.113~118 (1998)
- Williams K, "Relationship between expression of sex steroid receptors and structure of the seminal vesicles after neonatal treatment of rats with potent or weak estrogens", Environ Health Perspect, 109, pp.1227~1235 (2001)
- Yamashita S, "Localization of estrogen and androgen receptors in male reproductive tissues of mice and rats", Anat Rec, 279A, pp.768~778 (2004)