형질전환생쥐의 제조 수단으로서 유전자 적중법 및 함정법의 개발 현황
A Current Advance of Gene Targeting and Gene Trapping Methods As Tools of Making Transgenic Mice
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The construction of transgenic mouse using embryonic stem (ES) cells has been crucial in the functional studies of gene on mouse genome. Gene knockout mice have been powerful for elucidating the function of genes as well as a research model for human diseases. Gene targeting and gene trapping mathods have been the representative technologies for making the knockout mice by using ES cells. Since the gene targeting and the gene trapping methods were independently developed about 20 years ago, it's efficiency and productivity has been improved with a advance of molecular biology. Conventional gene targeting method has been changes to high throughput conditional gene targeting. The combination of the advantage of gene targeting and gene tapping elements allows to extend a spectrum of gene trapping and to improve the efficiency of gene targeting. These advance should be able to produce the mutant with various phenotype to target a certain gene, and in postgenome era they have served as crucial research tools in understanding the functional study of whole genome in mouse.
Abstract
The construction of transgenic mouse using embryonic stem (ES) cells has been crucial in the functional studies of gene on mouse genome. Gene knockout mice have been powerful for elucidating the function of genes as well as a research model for human diseases. Gene targeting and gene trapping mathods have been the representative technologies for making the knockout mice by using ES cells. Since the gene targeting and the gene trapping methods were independently developed about 20 years ago, it's efficiency and productivity has been improved with a advance of molecular biology. Conventional gene targeting method has been changes to high throughput conditional gene targeting. The combination of the advantage of gene targeting and gene tapping elements allows to extend a spectrum of gene trapping and to improve the efficiency of gene targeting. These advance should be able to produce the mutant with various phenotype to target a certain gene, and in postgenome era they have served as crucial research tools in understanding the functional study of whole genome in mouse.
참고문헌 (46)
- Abuin A, "Gene trap mutagenesis", Handb Exp Pharmacol, 178, pp.129~147 (2007)
- Araki K, "Exchangeable gene trap using the Cre", mutated lox system, 1999, pp.45, 737~750
- Austin CP, "The knockout mouse project", Nat Genet, 36, pp.921~924 (2004)
- Bestor TH, "Transposons reanimated in mice", Cell, 122, pp.322~325 (2005)
- Brown SD, "The mouse genome", Genome Dyn, 2, pp.33~45 (2006)
- Capecchi MR, "Altering the genome by homologous recombination", Science, 244, pp.1288~1292 (1989)
- Capecchi MR, "Generating mice with targeted mutations", Nat Med, 7, pp.1086~1090 (2001)
- Carlson CM, "Somatic integration of an oncogene-harboring Sleeping Beauty transposon models liver tumor development in the mouse", Proc Natl Acad Sci USA, 통권 : 102, pp.17059~17064 (2005)
- Copeland NG, "Recombineering: A powerful new tool for mouse functional genomics", Nat Rev Genet, 2, pp.769~779 (2001)
- Ding S, "Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice", Cell, 122, pp.473~483 (2005)
- Dupuy AJ, "Transposition and gene disruption in the male germline of the mouse", Genesis, 30, pp.82~88 (2001)
- Friedel RH, "Gene targeting using a promoterless gene trap vector ("targeted trapping") is an efficient method to mutate a large fraction of genes", Proc Natl Acad Sci USA, 통권 : 102, pp.13188~13193 (2005)
- Friedrich G, "Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice", Genes & Development, 5, pp.1513~1523 (1991)
- Gossler A, "Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes", Science, 244, pp.463~465 (1989)
- Glaser S, "Current issues in mouse genome engineering", Nat Genet, 37, pp.1187~1193 (2005)
- Hacking DF, ""Knock, and it shall be opened": Knocking out and knocking in to reveal mechanisms of disease and novel therapies", Early Hum Dev, 84, pp.821~827 (2008)
- Henikoff S, "TILLING. Traditional mutagenesis meets functional genomics", Plant Physiol, 135, pp.630~636 (2004)
- Ishida Y, "RET: A poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells", Nucleic Acids Res, 27, pp.e35~ (1999)
- Ivics Z, "Transposable elements for transgenesis and insertional mutagenesis in vertebrates: A contemporary review of experimental strategies", Methods Mol Biol, 260, pp.255~276 (2004)
- Kang HM, "Dicistronic tagging of genes active in embryonic stem cells of mice", Mol Cells, 7, pp.502~508 (1997)
- Kothary R, "A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube", Nature, 335, pp.435~437 (1988)
- Lee T, "Gene trap mutagenesis: A functional genomics approach towards reproductive research", Mol Hum Reprod, 13, pp.771~779 (2007)
- Liu P, "A highly efficient recombineering-based method for generating conditional knockout mutations", Genome Res, 13, pp.476~484 (2003)
- Mansour SL, "Disruption of the protooncogene int-2 in mouse embryo- derived stem cells: A general strategy for targeting mutations to non-selectable genes", Nature, 336, pp.348~352 (1988)
- McCarrick JW III, "Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells", Transgenic Res, 2, pp.183~190 (1993)
- Michael SK, "Efficient gene-specific expression of cre recombinase in the mouse embryo by targeted insertion of a novel IRES-Cre cassette into endogenous loci", Mech Dev, 85, pp.35~37 (1999)
- Nagy A, "Creation and use of a cre recombinase transgenic database", Methods Mol Biol, 530, pp.1~4 (2009)
- Niwa H, "Mouse ES cell cuture system as a model of development", Develop Growth Differ, 52, pp.275~283 (2010)
- Niwa H, "An efficient gene-trap method using poly A trap vectors and characterization of gene-trap events", J Biochem, 113, pp.343~349 (1993)
- O’Kane CJ, "Detection in situ of genomic regulatory elements in Drosophila", Proc Natl Acad Sci USA, 통권 : 84, pp.9123~9127 (1987)
- Schnutgen F, "Adopting the good reFLEXes when generating conditional alterations in the mouse genome", Transgenic Res, 16, pp.405~413 (2007)
- Schnutgen F, "Enhanced gene trapping in mouse embryonic stem cells", Nucleic Acids Res, 36, pp.e133~ (2008)
- Shigeoka T, "Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells", Nucleic Acids Res, 33, pp.e20~ (2005)
- Skarnes WC, "A gene trap approach in mouse embryonic stem cells: The lacZ reporter is activated by splicing, reflects endogenous gene expression, and is mutagenic in mice", Genes & Development, 6, pp.903~918 (1992)
- Sun LV, "PBmice: An integrated database system of piggyBac (PB) insertional mutations and their characterizations in mice", Nucleic Acids Res, 36, pp.D729~D734 (2008)
- Szybalski W, "Use of the HPRT gene and the HAT selection technique in DNA-mediated transformation of mammalian cells: First steps toward developing hybridoma techniques and gene therapy", Bioessays, 14, pp.495~500 (1992)
- Testa G, "A reliable lacZ expression reporter cassette for multipurpose, knockout-first alleles", Genesis, 38, pp.151~158 (2004)
- Thomas KR, "Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells", Cell, 51, pp.503~512 (1987)
- Vivian JL, "An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl- N-nitrosourea-mutagenized mouse embryonic stem cells", Proc Natl Acad Sci USA, 통권 : 99, pp.15542~15547 (2002)
- Wang W, "A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells", Genome Res., 19, pp.667~673 (2009)
- Wu S, "Toward simpler and faster genome-wide mutagenesis in mice", Nat Genet, 39, pp.922~930 (2007)
- Yagi T, "A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene", Anal Biochem, 214, pp.77~86 (1993)
- Zambrowicz BP, "Comprehensive mammalian genetics: History and future prospects of gene trapping in the mouse", Int J Dev Biol, 42, pp.1025~1036 (1998)
- Zambrowicz BP, "Disruption and sequence identification of 2,000 genes in mouse embryonic stem cells", Nature, 392, pp.608~611 (1998)
- Zheng B, "A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice", Nucleic Acids Res, 27, pp.2354~2360 (1999)
- Zijlstra M, "β2-microglobulin deficient mice lack CD4-8+ cytotoxic T cells", Nature, 344, pp.742~746 (1990)