수중 및 균형 운동이 파킨슨 유발 백서모델에서 중뇌 흑질의 Tyrosine Hydroxylase 발현과 기능 회복에 미치는 영향
Influence of Aqua and Balance Exercise on Tyrosine Hydroxylase Expression in the Substantia Nigra and Functional Recovery in Hemiparkinsonian Rat Model
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이 연구는 수중 운동, 균형 훈련과 복합 운동이 파킨슨 유발 흰쥐 모델에 미치는 영향을 관찰하기 위해 수산화도파민(6-hydroxydopamine)을 흰쥐의 좌측 내측전뇌다발에 주사하여 파킨슨 모델을 제작하였다. 실험동물은 수중 운동을 적용한 실험군 Ⅰ, 균형 훈련을 적용한 실험군 Ⅱ, 복합 운동(수중 운동+균형 훈련)을 적용한 실험군 Ⅲ, 수술 후 표준사육장에서 운동을 적용하지 않은 대조군으로 배치하였다. 수술 후 7일, 14일, 21일에 실험동물의 중뇌 흑질의 도파민 세포 감소를 관찰하기 위하여 TH(tyrosine hydroxylase)의 발현 양상을 관찰하였고, 행동학적 검사로 수술 후 1일, 7일, 14일, 21일에 아포몰핀유도 회전 검사를 실시하였다. 실험 결과, 실험군들에서 대조군에 비하여 유의하게 높은 TH 발현과 아포몰핀유도 회전수를 보였다. 하지만 실험군들 사이에는 유의한 차이가 나타나지 않았다. 이상의 결과에서 파킨슨유발 흰쥐 모델이 기능 회복에 수중 운동과 균형 훈련이 효과적임을 알 수 있었으며, 흑질 치밀부의 도파민성 신경섬유의 증감을 나타내는 TH의 발현에도 수중 운동과 균형 훈련이 효과적임을 알 수 있었다.
Abstract
The objective of this study was to determine the effect of exercise on a hemiparkinsonian rat model. Nigrostriatal dopamine cell lesions were produced by injecting 6-hydroxydopamine at the left medial forebrain bundle of rats. In this study, the rats were divided into the following 4 groups: the control group without any exercise, experimental group Ⅰ with aqua-exercise (ExpⅠ), experimental group Ⅱ with balance exercise (ExpⅡ) and experimental group Ⅲ with complex exercise (aqua-exercise＋balance exercise; (ExpⅢ)). Exercises were applied to all the experimental groups after the operation. In order to observe the dopaminergic cell loss, we assessed the level of tyrosine hydroxylase (TH) expression in the midbrain of rats, and performed the apomorphine-induced rotation test at postoperative days (PDs) 7, 14, and 21. Experimental groups had significantly higher TH-immunoreactivity and behavioral performance than the control group. However, there was no difference in TH-immunoreactivity and behavioral performance across the experimental groups. These results suggest that the application of aqua-exercise and balance exercise could suppress dopaminergic cell loss in the substantia nigra of rat brains and could increase behavioral recovery in hemiparkinsonian rats.
참고문헌 (27)
- Anglade P, "Plasticity of nerve afferents to nigrostriatal neurons in Parkinson's disease", Ann Neurol, 37, pp.265~272 (1995)
- Bezard E, "Compensatory mechanisms in experimental and human Parkinsonism: towards a dynamic approach", Prog Neurobiol, 55, pp.93~116 (1998)
- Deumens R, "Review: Modeling Parkinson's disease in rats: an evaluation of 6-OHDA lesion of the nigrostriatal pathway", Exp Neurol, 175, pp.303~317 (2002)
- Ding Y, "Motor balance and coordination training enhances functional outcome in rat with transient middle cerebral artery occlusion", Neurosci, 123, pp.667~674 (2004)
- Edelman GM, "Degeneracy and complexity in biological systems", Proc Natl Acad Sci U.S.A, 98, pp.13763~13768 (2001)
- Elbaz A, "Risk tables for parkinsonism and Parkinson's disease", J Clin Epidemiol, 55, pp.25~31 (2002)
- Eriksen JL, "Parkinson's disease - molecular mechanisms of disease", J Clin Epidemiol, 55, pp.25~31 (2004)
- Foley P, "Pathogenesis and preclinical course of Parkinson's disease", J Neural Transm, 56, pp.31~74 (1999)
- Goede CJT, "The effects of physical therapy in Parkinson's disease: a research synthesis", Arch Phys Med Rehabil, 82, pp.509~515 (2001)
- Haavik J, "Tyrosine hydroxlase and Parkinson's disease", Mol Neurobiol, 16, pp.285~309 (1998)
- Helmich RC, "Cerebral compensation during motor imagery in Parkinson's disease", Neuropsychologia, 45, pp.2201~2215 (2007)
- Howells FM, "Stress reduces the neuroprotective effect of exercise in a rat model for Parkinson's disease", Behav Brain Res, 165, pp.210~220 (2005)
- Keus, SHJ, "Effectiveness of physiotherapy in Parkinson's disease: the feasibility of a randomized controlled trial", Parkinsonism Relat Disord, 13, pp.115~121 (2007)
- Mariani E, "Oxidative stress in brain aging, neurodegenerative and vascular disease: an overview", J Chromatogr, 827, pp.65~75 (2005)
- Palop JJ, "A network dysfunction perspective on neurodegenerative diseases", Nature, 443, pp.768~773 (2006)
- Poulton NP, "Treadmill training ameliorates dopamine loss but not behavioral deficits in hemiparkinsonian rats", Exp Neurol, 193, pp.181~197 (2005)
- Price CJ, "Functional imaging studies of neuropsychological patients: applications and limitations", Neurocase, 8, pp.345~354 (2002)
- Reuter I, "Therapeutic value of exercise training of Parkinson's disease", Med Sci Sports Exerc, 31, pp.1544~1549 (1999)
- Savitt JM, "Diagnosis and treatment of Parkinson disease: molecules to medicine", J Clin Invest, 116, pp.17544~11754 (2006)
- Seeman P, "Dopamine receptors and transporters in Parkinson's disease and schizophrenia", FASEBJ, 4, pp.2737~2744 (1990)
- Shimohama S, "Disease model: Parkinson's disease", Trends Mol Med, 9, pp.360~365 (2003)
- Singh N, "Advance in the treatment of Parkinson's disease", Prog Neurobiol, 81, pp.29~44 (2007)
- Smith AD, "Effect of bilateral 6-hydroxydopamine lesions of the medial forebrain bundle on reaction time", Neuropsychopharmacol, 26, pp.756~764 (2002)
- Tilerson JL, "Exercise induces behavioral recovery and attenuates neurochemical deficits in rodents models of Parkinson's disease", Neurosci, 119, pp.899~911 (2003)
- Yoon MC, "Treadmill exercise suppresses nigrostriatal dopaminergic neuronal loss in 6-hydroxydopamine-induced Parkinson's rats", Neurosci Lett, 423, pp.12~17 (2007)
- 송주민, "외상성 소뇌 손상 흰쥐에서 환경강화가 운동기능과 BDNF 발현에 미치는 영향", 박사, pp.2003
- 허현진, "수컷 흰쥐의 시상하부-뇌하수체 축 호르몬 유전자 발현에 미치는 6-Hydroxydopamine(6-OHDA)의 영향", 발생과 생식, 13(4), pp.257~264 (2009), 한국발생생물학회