트레드밀 운동이 6-OHDA로 유발된 파킨슨병 흰쥐 흑색질 내 염증지표에 미치는 영향
Effect of treadmill exercise on inflammatory markers in the substantia nigra in 6-OHDA-induced Parkinson's rats.
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본 연구에서는 트레드밀 운동이 파킨슨병 쥐의 흑색질에서 염증반응에 미치는 영향을 실험하였다. 6주령의 수컷 쥐를 사용하였으며, 파킨슨병은 뇌정위 수술(stereotaxic surgery) 후 6-hydroxydopamine(6-OHDA)를 주입하여 유발하였다. 각각 가성수술군(sham-operation group; n=10), 가성수술 운동군(sham-operation and exercise group; n=10), 파킨슨병군(6-OHDA-injection group; n=10), 파킨슨병 운동군(6-OHDA-injection and exercise group; n=10)으로 나누어 파킨슨병 유발 4주후부터 운동군은 하루 30분 총 14일간 트레드밀 달리기를 하였다. 흑색질의 염증반응은 cyclooxygenase-2(COX-2) 및 inducible nitric oxide synthase (iNOS) 면역조직화학법으로 분석하였다. 흑색질의 COX-2 양성세포수는 가성수술군이 54.19±3.68, 운동군이 42.94±3.01, 파킨슨병 유발군이 98.22±4.73, 파킨슨병 유발 운동군이 61.78±3.86을 나타내었으며. iNOS 양성세포수는 가성수술군이 57.93±3.38, 운동군이 51.64±5.17, 파킨슨병 유발군이 126.56±7.04, 파킨슨병 유발 운동군이 92.69±8.28을 나타내었다. 본 실험을 통하여 6-OHDA 처치로 인해 염증반응이 증가하였으며, 트레드밀 운동이 COX-2 및 iNOS의 발현을 유의하게 감소시켜. 운동이 파킨슨병으로 인한 뇌신경세포의 염증반응을 완화시키는 긍정적인 효과를 보이는 것을 알 수 있었다.
Abstract
In the present study, we investigated the effects of treadmill exercise on inflammatory reaction in the substantia nigra of the Parkinson's rats. Parkinson's disease was made by injection of 6-hydroxydopamine (6-OHDA) into the striatum using 6-week-old male rats. The rats were divided into 4 groups: sham-operation group (n=10), sham-operation and exercise group (n=10), 6-OHDA-injection group (n=10), and 6-OHDA-injection and exercise group (n=10). The animals in the exercise groups were put on the treadmill to run for 30 minutes once a day for 14 consecutive days, starting 4 weeks after 6-OHDA injection. For the detection of inflammatory reaction in the substantia nigra, immunohistochemistry for cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was performed. The number of COX-2-positive cells in substantia nigra was 54.19±3.68 in the sham-operation group, 42.94±3.01 in the sham-operation and exercise group, 98.22±4.73 in the 6-OHDA-injection group, and 61.78±3.86 in the 6-OHDA-injection and exercise group. The number of iNOS-positive cells in substantia nigra was 57.93±3.38 in the sham-operation group, 51.64±5.17 in the sham-operation and exercise group, 126.56±7.04 in the 6-OHDA-injection group, and 92.69±8.28 in the 6-OHDA-injection and exercise group. In the present results, 6-OHDA injection increased inflammatory reaction in the substantia nigra, and treadmill exercise alleviated the expression of COX-2 and iNOS in the substantia nigra. These results suggest that treadmill exercise may ameliorate inflammatory reaction in the Parkinson's disease patients.
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