Induction of Primary Male in Juvenile Red Spotted Grouper Epinephelus akaara by Immersion of 17α-Methyltestosterone
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초록
We investigated the androgenic effects of 17α- methyltestosterone (MT) on gonadal sex reversal in juvenilered spotted grouper Epinephelus akaara. The fish were immersed in 17α-MT at 1 and 5 mg/L. Treatment method of 17α-MTwas once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end of the treatment period in order to histologicalanalysis. At the initiation of an experiment (70 day after hatching), juvenile red spotted grouper have the paired primordialgonads with somatic cells bellow kidney in the posterior portion of the body cavity. Formation of ovarian cavity indicates thatthe ovarian differentiation beginning at 70 DAH in red spotted grouper. At 12 months after end of the treatment period,control group, 17α-MT 1 mg/L treatment group for 4 and 8 weeks, and 17α-MT 5 mg/L treatment group for 4 weeks were allfemale. However, sex-changed males without ovarian cavity were observed in the 17α-MT 5 mg/L treatment group for 8weeks. In grouper, we firstly reported that the red spotted grouper be able to induce the primary males by hormone treatmentprior to gonadal sex differentiation.
Abstract
We investigated the androgenic effects of 17α- methyltestosterone (MT) on gonadal sex reversal in juvenilered spotted grouper Epinephelus akaara. The fish were immersed in 17α-MT at 1 and 5 mg/L. Treatment method of 17α-MTwas once weekly for 4 and 8 weeks. Fish were sampled at 12 months after end of the treatment period in order to histologicalanalysis. At the initiation of an experiment (70 day after hatching), juvenile red spotted grouper have the paired primordialgonads with somatic cells bellow kidney in the posterior portion of the body cavity. Formation of ovarian cavity indicates thatthe ovarian differentiation beginning at 70 DAH in red spotted grouper. At 12 months after end of the treatment period,control group, 17α-MT 1 mg/L treatment group for 4 and 8 weeks, and 17α-MT 5 mg/L treatment group for 4 weeks were allfemale. However, sex-changed males without ovarian cavity were observed in the 17α-MT 5 mg/L treatment group for 8weeks. In grouper, we firstly reported that the red spotted grouper be able to induce the primary males by hormone treatmentprior to gonadal sex differentiation.
참고문헌 (23)
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