Effect of 11-ketotestosterone (11-KT) on Gonadal Sex Reversal and Spermatogenesis of Honeycomb Grouper Epinephelus merra
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We investigated the androgenic effects of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis in honeycomb grouper Epinephelus merra by method of gonadal biopsy. 11-KT was injected intramuscularly at a concentration of 1 and 10 ㎍/g body weight. The proportion of cross sectional area of the gonad occupied by each germ cell type was measured and compared pre- and post-injection group. During the sex change phase, the distribution ratio of oocytes was decreased in all fish of 11-KT treatment group while the distribution ratio of spermatocytes was increased than pre-injection group. In male phase, all fish of 11-KT treatment group shown the increased distribution ratio of spermatocytes, but the distribution ratio of spermatozoa was decreased than pre-injection group. The present results suggest that 11-KT can stimulate degeneration of oocytes, proliferation of spermatocytes and spermiation in honeycomb grouper.
Abstract
We investigated the androgenic effects of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis in honeycomb grouper Epinephelus merra by method of gonadal biopsy. 11-KT was injected intramuscularly at a concentration of 1 and 10 ㎍/g body weight. The proportion of cross sectional area of the gonad occupied by each germ cell type was measured and compared pre- and post-injection group. During the sex change phase, the distribution ratio of oocytes was decreased in all fish of 11-KT treatment group while the distribution ratio of spermatocytes was increased than pre-injection group. In male phase, all fish of 11-KT treatment group shown the increased distribution ratio of spermatocytes, but the distribution ratio of spermatozoa was decreased than pre-injection group. The present results suggest that 11-KT can stimulate degeneration of oocytes, proliferation of spermatocytes and spermiation in honeycomb grouper.
참고문헌 (21)
- Alam MA, "Changes in androgen- producing cell size and circulating 11-ketotestosterone level during female-male sex change of honeycomb grouper Epinephelus merra", Mol Reprod Dev, 73, pp.206~214 (2006)
- Bhandari RK, "Gonadal restructuring and correlative steroid hormone profiles during natural sex change protogynous honeycomb grouper (Epinphelus merra)", Zool Sci, 20, pp.1399~1404 (2003)
- Borg B, "Androgens in teleost fishes", Comp Biochem Physiol, 109C, pp.219~245 (1994)
- Cardwell JR, "Hormonal control of sex and color change in the spotlight parrotfish, Sparisoma viride", Gen Comp Endocrinol, 81, pp.7~20 (1991)
- Fostier A, "The gonadal steroids. in: Fish Physiology, vol. IXA", pp.277~372 (1983), Academic Press
- Fostier A, "Steroids in male reproduction. In: proc. Third International Symposium on Reproductive Physiology of Fish", pp.239~245 (1988), University of St-Johns
- Grober MS, "Gonadal steroid affects LHRH preoptic cell number in a sex", role changing fish, 1991, pp.22, 734~741
- Kobayashi Y, "Histological observation of the sex change in honeycomb grouper, Epinephelus merra", proc. 6th International Symposium on the Reproductive Physiology of Fish, pp.269~ (2000), Institute for Marine Research and University of Bergen
- Kokokiris L, "Gonadal changes and blood sex steroids levels during natural sex inversion in the protogynous Mediterranean red porgy Pagrus pagrus (Teleostei: Sparidae)", Gen Comp Endocrinol, 149, pp.42~48 (2006)
- Kroon FJ, "The role of steroid hormones in protogynous sex change in the blackeye goby, Coryphopterus nicholsii (TeleosteiL Gobiidae)", Gen Comp Endocrinol, 118, pp.273~283 (2000)
- Miura T, "Japanese eel: a model for analysis of spermatogenesis", Zool Sci, 18, pp.1055~1063 (2001)
- Miura T, "Molecular control mechanisms of fish spermatogenesis", Fish Physiol Biochem, 28, pp.181~186 (2003)
- Miura T, "Induction of spermatogenesis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gonadotropin", Zool Sci, 8, pp.63~73 (1991)
- Mohamed AA, "Involvement of sex steroid hormones in the early stages of spermatogenesis in Japanese Huchen (Hucho perryi)", Biol Reprod, 65, pp.1057~1066 (2001)
- Nakamura M, "Effects of 11-ketotestosterone on gonadal sex differentiation in Tilapia mossambica", Bulletin Japanese Society of Scientific Fisheries, 47(10), pp.1323~1327 (1981)
- Nakamura M, "Histological and ultrastructural evidence for the role of gonadal steroids hormones in sex change in the protogynous wrasse Thalassoma duperrey", Env Biol Fish, 24, pp.117~136 (1989)
- Ozon R, "Androgens in fishes, amphibians, reptiles and birds. in: Steroids in Non Mammalian Vertebrates", pp.329~389 (1972), Academic Press
- Schulz RW, "Spermatogenesis and its endocrine regulation", Fish Physiol Biochem, 26, pp.43~56 (2002)
- Takahashi H, "Masculinization of the gonad of juvenile guppy, Poecilia reticulate, induced by 11-ketotestosterone", Bull Fac Fish Hokkaido Univ, 26, pp.223~234 (1975)
- Tanaka H, "Induction of sex reversal in the sevenband grouper, Epinephelus septemfasciatus", proc. 6th International Symposium on the Reproductive Physiology of Fish, pp.423~ (2000), Institute for Marine Research and University of Bergen
- Yeh SL, "Androgens stimulate sex change in protogynous grouper, Epinephelus coioides: spawning performance in sex-changed males", Comp Biochem Physiol Part C, 135, pp.375~382 (2003)