Improvement of the Vitrification Method Suppressing the Disturbance of Meiotic Spindle and Chromosome Systems in Mature Oocytes
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Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than thatof the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome of matureoocyte. To get the best way for keeping competence of matured oocytes, we studied to get the best conditions for vitrificationfocused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzedthe survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same betweengroups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration groupand the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimentalgroups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 minequilibration group showed best condition compared with the other groups. Based on these results, the equilibration time isone of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effectsof physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimalequilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight intointracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of humanoocyte.
Abstract
Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than thatof the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome of matureoocyte. To get the best way for keeping competence of matured oocytes, we studied to get the best conditions for vitrificationfocused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzedthe survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same betweengroups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration groupand the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimentalgroups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 minequilibration group showed best condition compared with the other groups. Based on these results, the equilibration time isone of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effectsof physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimalequilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight intointracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of humanoocyte.
참고문헌 (36)
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