고추의 DUS 검정을 위한 AFLP 분석의 이용
Application of Amplified Fragment Length Polymorphism (AFLP) Analysis for DUS Test in Pepper Cultivars
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Abstract
This study was carried out to assess the potential of AFLP markers for testing distinctness, uniformity and stability ofpepper (Capsicum annuum L.) cultivars. Sixty-six cultivars were analyzed by AFLP using EcoRI/MseI primer combinations.Nineteen combinations of AFLP primer led to the amplification of 125 scorable fragments ranging from 79 to 748 bp and amongrated into seven distinctive groups corresponding to varietal types and genetic distance of cluster ranging from 0.48 and 1.00. Therelationship between morphological characters and genetic similarity through AFLP analysis was analyzed. The molecular geneticdistance was consistent with the morphological traits measured for identification and distinctiveness tests.