AFLP 분석을 이용한 무와 배추의 품종식별

Abstract

In this study, we have adopted AFLP (Amplified Fragment Length Polymorphism) analysis procedure for discrimina-tion of cultivars of radish (Raphanus sativus. L) and Chinese cabbage (Brassica campestris ssp. pekinensis). In radish, 123 polymo-combinations. The number of average polymorphism obtained per each primer combination was 5.5 and 7.6 in radish and Chinesecabbage, respectively. In addition, standard varieties were provided to all markers for both crops to secure availability in futurework. By cluster analysis, radish cultivars were classified by root shape and cultivation season, while those of Chinese cabbagewere grouped in part by wintering. Moreover, the results showed considerable differences between recently applied varieties andcurrent existing varieties, which meant that recently bred new cultivars have distinguished phenotypes from current existing variet-ies. Practically, the number of primers required for variety discrimination was determined as 12~18 and 6~10 for radish and Chi-nese cabbage, respectively. Collectively, our results of AFLP analysis on radish and Chinese cabbage were proved to be useful indefining and identification of varieties.