AFLP marker를 이용한 콩의 유전적 다양성과 유전분리 분석
Diversity and Inheritance of AFLP Markers in Wild and Cultivated Soybeans
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Abstract
Genetic variation is the basis of crop improvement. Limited genetic diversity in a cropspecies may restrict the amount of genetic improvement that can be achieved through plantbreeding. Soybean is one of the world’s most important crops. A potential source of geneticvariability for the cultivated soybean is the wild species G. soja Sieb. & Zucc. Amplifiedfragment length polymorphism (AFLP) analysis is a PCR-based technique, which can detecta 10-fold greater nubmer of loci than other DNA marker analysis. Twenty cultivatedsoybeans and two-hundred wild soybeans were used to determine genetic vatiations byAFLPs and evaluate the usefulness of AFLPs as DNA markers. Six-hundred and tenfragments were detected with an average of 56 AFLP fragments produced per primer in atotal of 11 AFLP primer pairs. The number of polymorphic loci detected per primer rangedfrom 7 to 20 and the polymorphism was greater in wild than in cultivated soybean. F2segregation analysis of four AFLP fragments in combination of Hwaeomputkong × PI417479 indicated that they segregate as stable Mendelian loci with 3 : 1. This resultsstrongly suggest that the AFLP analysis is a good technique for the detection of geneticpolymorphism in a wide plant species.
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