일품벼 X 모로베레칸 조합 여교잡 BC3F2 계통 특성 평가
Evaluation of Agronomic Traits of an Ilpumbyeo ´ Moroberekan BC3F2 Population
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본 실험은 양질이지만 도열병에 이병성인 일품벼와 지속적 저항성인 모로베레칸과의 3차례의 여교잡과 DNA 표지를 이용하여 도열병 저항성인 일품벼 근동질 유전자계통을 육성하기 위해 실시되었다. 육성된 BC3F2 계통들은 일부 계통을 제외하고는 출수기, 간장 등 주요 농업형질에서 일품벼와 유사하였다. 이입된 모로베레칸의 유전자와 조사된 형질과의 관련성 여부를 파악하기 위해 QTL 분석을 실시하였다. 일품벼로 이전된 모로베레칸의 염색체 단편들이 몇몇 형질에 영향을 미쳤는데 QTL 분석 결과 출수기 관련 QTLs은 1, 4 및 6번 염색체에서, 간장 관련 QTLs은 각각 1, 7 및 8번 염색체에서 1개씩 탐지되었다. 수장 및 수수와 관련된 QTL이 모두 1번과 6번 염색체에서 각각 2개씩, 천립중에 관련된 QTL은 2번 염색체에서 1개 탐지되었고, 영색에 관여하는 유전자(gh-2)는 2번 염색체에서 탐지되었다. 육성된 여교잡계통은 일품벼의 유전적 배경에 모로베레칸의 서로 다른 염색체단편이 이입된 근동질 유전자계통으로 앞으로 도열병저항성, 미질 특성 등에 대한 정밀한 검정이 요구되며, 검정 결과 미질이 양호하고 도열병에 대해 고도의 저항성을 보이는 계통들은 중간모본, 품종 및 다계품종 등으로 직접 이용하거나, 도열병 저항성 유전자 분석을 위한 판별품종 및 내구저항성 유전자의 정확한 평가 및 특성 검정에 활용할 것이다.
Abstract
During the progress of introgressive backcrossing between Ilpumbyeo as a recurrent parent and Moroberekan as a donor to develop promising lines resistant to rice blast, 279 BC3F1 plants which were phenotypically similar to Ilpumbyeo and resistant to blast were selected. These 279 BC3F2 lines were evaluated for several agronomic traits in the field and also were genotyped with 99 SSR markers evenly distributed on the rice chromosome to detect Moroberekan chromosomal segments. These lines displayed similar phenotypes compared to Ilpumbyeo in evaluated traits including days to heading. Several lines showed differences compared to Ilpumbyeo and these differences might be due to the Moroberekan chromosome segments introgressed into Ilpumbyeo. To know the effects of the Moroberekan chromosomal segments on the traits, QTL analysis was carried out using one-way ANOVA.. Three QTLs related to days to heading were detected on chromosomes 1, 4 and 6. QTLs related to culm length were detected on chromosomes 1,7 and 8. Two QTLs each for panicle length and panicle number were detected on chromosomes 1 and 6, respectively. One QTL for 1,000-grain weight and the gene for gold hull were mapped on chromosome 2, respectively. The number of Moroberekan fragments in the 279 NILs was in the range of 3 - 16 and the length of introgressed fragments was in the range of 3.9 - 112.1 cM. These NILs developed here will be useful for breeding resistant cultivars and characterization of blast resistance.
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