벼멸구 저항성 여교잡 집단의 등숙특성 QTL 분석
QTL Analysis for Ripening Traits of BHP Resistant Backcross Inbred Lines in Rice
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본 실험은 벼멸구 저항성과 등숙 관련 형질과의 연관관계를 구명하고자 5회 여교배된 130 BIL계통들을 이용하여 QTL분석을 수행하였다. 벼멸구 저항성 계통들은 감수성 계통들에 비해 등숙율 및 현미 완전미율이 유의하게 높았으며, 수당립수는 감소하였다. QTL 분석에서 등숙율과 현미완전미율은 벼멸구 저항성 유전자가 포함된 RM3813~RM5609사이에서 QTL이 탐색되었다. 등숙관련 QTL (gfr12)와 현미 완전미율 관련 QTL (bhr12)은 각각 전체 표현형 변이의 23%와 21%를 설명 가능한 것으로 분석되었다. 등숙율과 현미 완전미율의 증가에는 삼강벼의 대립유전자가 관여하는 것으로 나타났다. 이들 결과에서 벼멸구 저항성 유전자와 등숙율 및 현미 완전미율에 관여하는 유전자들은 서로 연관되어 있거나, 동일한 유전자가 관여할 것으로 추정된다. 따라서 벼멸구 저항성을 가지면서 쌀의 외관특성이 양호한 고품질 벼 품종의 육성이 가능 할 것으로 사료되었다.
Abstract
In order to identify the relationship between a brown planthopper (Nilaparvata lugens Stal) resistance gene Bph1 andripening traits, we conducted the QTL analysis using 130 backcross inbred lines (BC5F5BIL) derived from a cross of the BPH sus-ceptible Japonica cultivar, Nagdongbyeo as the recurrent parent and Tongil-type resistant cultivar Samgangbyeo as a donor par-ingrain filling ratio, head rice ratio of brown rice and number of spikelets per panicle. Five SSR markers on chromosome 12 detectedin the introgressed chromosome segment of resistant donor, and two QTLs related with grain filling ratio (gfr12) and brown headrice ratio (bhr12) were detected in the marker between RM3813 and RM5609 explaining 23% and 21% of the total variance,respectively. Grain filling ratio and head rice ratio of brown rice were increased by Samgangbyeo allele. This result suggests thathigh quality variety carrying the BPH resistance gene could be developed by combination of MAS and backcross in rice breeding.
참고문헌 (11)
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