일품벼/모로베레칸 이입계통을 이용한 농업형질 관련 QTL 분석
Mapping QTLs for Agronomic Traits Using an Introgressin Line Population from a Cross between Ilpumbyeo and Moroberekan in Rice
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We conducted a QTL analysis of agronomic traits using 117 BC3F5 and BC3F6 lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 BC3F5 lines were determined using 134 simple sequence repeat (SSR) markers. A total of 832 Moroberekan chromosome segments with 410 homozygous and 422 heterozygous, respectively, were detected, and the genetic distance of introgression segments ranged from 0.5 cm to 112.1 cm. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 BC3F5 and BC3F6 lines were evaluated for seven agronomic traits at two locations in 2006 and 2007 and at one location in 2007. A total of 26 QTLs were identified for seven traits including days to heading, and the phenotypic variance explained by each QTL ranged from 9.2% to 24.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at eleven QTL loci including panicle length and spikelets per panicle. Five QTLs, two for days to heading and one each for culm length, panicle length and spikelets per panicle were consistently detected in every occasions indicating that these QTLs are stable. Among them, two QTLs, spp6 for spikelets per panicle and pl6 for paniclel length were localized in the similar region. Increase in spikelets per panicle at this locus might be due to the increase in panicle length, because both traits were associated with increase in spikelets per panicle and panicle length due to the presence of the Moroberekan allele. These Moroberekan QTLs might be useful in breeding programs to develop high-yielding cultivars.
Abstract
We conducted a QTL analysis of agronomic traits using 117 BC3F5 and BC3F6 lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 BC3F5 lines were determined using 134 simple sequence repeat (SSR) markers. A total of 832 Moroberekan chromosome segments with 410 homozygous and 422 heterozygous, respectively, were detected, and the genetic distance of introgression segments ranged from 0.5 cm to 112.1 cm. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 BC3F5 and BC3F6 lines were evaluated for seven agronomic traits at two locations in 2006 and 2007 and at one location in 2007. A total of 26 QTLs were identified for seven traits including days to heading, and the phenotypic variance explained by each QTL ranged from 9.2% to 24.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at eleven QTL loci including panicle length and spikelets per panicle. Five QTLs, two for days to heading and one each for culm length, panicle length and spikelets per panicle were consistently detected in every occasions indicating that these QTLs are stable. Among them, two QTLs, spp6 for spikelets per panicle and pl6 for paniclel length were localized in the similar region. Increase in spikelets per panicle at this locus might be due to the increase in panicle length, because both traits were associated with increase in spikelets per panicle and panicle length due to the presence of the Moroberekan allele. These Moroberekan QTLs might be useful in breeding programs to develop high-yielding cultivars.
참고문헌 (20)
- Causse MA, "Saturated molecular map of the rice genome based on an interspecific backcross population", Genetics, 138, pp.1251~1274 (1994)
- Gur A, "Unused natural variation can lift yield barriers in plant breeding", PLoS Biology, 2(10), pp.1610~1615 (2004)
- 구홍광, "일품벼 X 모로베레칸 조합 여교잡 BC3F2 계통 특성 평가", 한국육종학회지, 36(5), pp.338~344 (2004), 한국육종학회
- Lanceras JC, "Quantitative trait Loci associated with drought tolerance at reproductive stage in rice", Plant Physiology, 135, pp.384~399 (2004)
- Li ZK, "Overdominant epistatic loci are the primary geneticbasis of inbreeding depression and heterosis in rice", I. Biomass and grain yield. Genetics, 158, pp.1737~1753 (2001)
- McCouch SR, "Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)", DNA Res, 9, pp.199~207 (2002)
- Moncada P, "Quantitative trait loci yield and yield components in an Oryza sativa X Oryza rufipogon BC2F2 population evaluated in an upland environment", Theor. Appl. Genet, 102, pp.41~52 (2001)
- Mu P, "QTL analysis for lodging resistance in rice using a DH population under lowland and upland ecosystems", Acta Genetica Sinica, 31, pp.717~723 (2004)
- Nelson JC, "Qgene manual. Electronic address: greengenes. Cit. cornell. Edu port 70; directory “Software for genetics", qGene.”, 1997
- Panaud O, "Development of microsatellite markers and characterization of simple sequence length polymorphism in rice (Oryza sativa)", Mol. Gen. Genet, 252, pp.597~607 (1996)
- Septimingsih EM, "Identification of quantitative trait loci for yield and yield components in an advaced backcross population derived from the Oryza sativa variety IR64 and the wild relative O. rufipogon", Theor. Appl. Genet, 107, pp.1419~1432 (2003)
- Tanksley SD, , Mapping polygenes. Ann. Rev. Genet, 27, pp.205~233 (1993)
- Temnykh S, "Computational and experimental analysis of microsatellite in rice (Oryza sativa L.): frequency length cariation, transposon associations, and genetic marker potential", Genome Res, 11, pp.1441~1452 (2001)
- Thomson MJ, "Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson", Theor. Appl. Genet, 107, pp.479~493 (2003)
- Tian F, "Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yield-related traits", Theor. Appl. Genet, 112, pp.570~580 (2005)
- Xiao JH, "Identification of trait- improving quantitative trait loci alleles from a wild rice relative, Oryza rufipogon", Genetics, 150, pp.899~909 (1998)
- Yamamoto T, "Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading of rice, as single mendelian factors", Theor. Appl. Genet, 97, pp.37~44 (1998)
- Yano M, "Genetic and moilecular dissection of naturally occurring varition", Curr. Opinion in Plant Biol, 4, pp.130~135 (2001)
- Yano M, "Genetic and molecular dissection of quantitative trait in rice", Plant Mol. Biol, 4, pp.130~135 (1997)
- Yano M, "Genetic control of flowering time in rice, a short-day plant", Plant Physiology, 117, pp.1425~1429 (2001)