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Introduction to Colorimetric Analysis for Assessment of Carotenoid Pigments in Squash Germplasm

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Carotenoids of squash play an important role in human health by acting as sources of provitamin A or as protective antioxidants. Among the 60 accessions of squash germplasm, fluorescent yellow and yellow types of flesh color got the highest count, followed by the orange, whitish yellow and greenish yellow. The redness and yellowness values of the flesh powder ranged from -2.45 to 86.09 and from 13.77 to 39.80, respectively. While the lightness and the total color difference values of flesh color varied from 67.64 to 86.09 and from 19.77 to 51.79, respectively. Colorimetric values of redness and yellowness showed positive correlation, and the correlation coefficient (r) was as high as 0.7386. The five accessions represented each flesh color types,IT195043 (orange), IT136696 (fluorescent yellow), IT186365 (yellow), IT137963 (whitish yellow), and IT180449 (greenish yellow). The total amount of carotenoid contents was in the order of orange color (104.64 mg/100 g), greenish yellow color (70.82 mg/100 g), fluorescent yellow color (32.41 mg/100 g), yellow color (8.73 mg/100 g), and whitish yellow color (4.73 mg/100 g). Both lutein and β-carotene were the predominant pigments of carotenoids, and lycopene was only separated and identified in the orange color flesh. According to the results, colorimetric analysis can aid breeders interested in increasing carotenoid content in squash, which could be accurately measured using a simple, reliable, and cost- and labor-efficient method for the evaluation of carotenoid pigments.

Abstract

Carotenoids of squash play an important role in human health by acting as sources of provitamin A or as protective antioxidants. Among the 60 accessions of squash germplasm, fluorescent yellow and yellow types of flesh color got the highest count, followed by the orange, whitish yellow and greenish yellow. The redness and yellowness values of the flesh powder ranged from -2.45 to 86.09 and from 13.77 to 39.80, respectively. While the lightness and the total color difference values of flesh color varied from 67.64 to 86.09 and from 19.77 to 51.79, respectively. Colorimetric values of redness and yellowness showed positive correlation, and the correlation coefficient (r) was as high as 0.7386. The five accessions represented each flesh color types,IT195043 (orange), IT136696 (fluorescent yellow), IT186365 (yellow), IT137963 (whitish yellow), and IT180449 (greenish yellow). The total amount of carotenoid contents was in the order of orange color (104.64 mg/100 g), greenish yellow color (70.82 mg/100 g), fluorescent yellow color (32.41 mg/100 g), yellow color (8.73 mg/100 g), and whitish yellow color (4.73 mg/100 g). Both lutein and β-carotene were the predominant pigments of carotenoids, and lycopene was only separated and identified in the orange color flesh. According to the results, colorimetric analysis can aid breeders interested in increasing carotenoid content in squash, which could be accurately measured using a simple, reliable, and cost- and labor-efficient method for the evaluation of carotenoid pigments.

참고문헌 (62)

  1. Ameny, MA, "Relationship between Hunter color values and β-carotene contents in whitefleshed African sweetpotatoes (Ipomoea batats Lam)", J. Sci. Food Agr, 73, pp.301~306 (1997)
  2. An, KB, "Research on characteristic factors of 63 native accessions Cucurbita moschata Duch. at central districts in Korea", Kyung-gi Agri. Research (KGARR), 7, pp.107~113 (1994)
  3. Andres, TC, "Biology and Utilization of the Cucurbitaceae", pp.102~119 (1990), Cornell Univ. Press
  4. Arias, R, "Correlation of lycopene measured by HPLC with the L*, a*, b* color reading of a hydroponic tomato and the relationship of maturity with color and lycopene content", J. Agr. Food Chem, 48, pp.1697~1702 (2000)
  5. Arima, HK, "Carotenoid composition and vitamin A value of commercial Brazilian squashes and pumpkins", J. Micronutrient Anal, 4, pp.177~191 (1988)
  6. Arvayo-Oetiz, RM, "Postharvest response of winter squash to hot-water treatment, temperature, and length of storage", Hort. Tech, 4, pp.253~255 (1994)
  7. Azevedo-Meleiro, CH, "Qualtative and quantitative differences in carotenoid composition among Cucurbita moschata, Cucurbita maxima, and Cucurbita pepo", J. Agric. Food Chem, 55, pp.4027~4033 (2007)
  8. Azizah, AH, "Effect of boiling and stir frying on total phenolics, carotenoids and radical scavenging activity of pumpkin (Cucurbita moschata)", Int. Food Res. J, 16, pp.45~51 (2009)
  9. Ben-amotz, A, "Analysis of carotenoids with emphasis on 9-cis β-carotene in vegetables and fruits commoly consumed in Israel", Food Chemi, 62, pp.515~520 (1998)
  10. Bognar, A, "Nutritive value of some varieties of pumpkin and winter squash growth in Germany", Ernahrungs Umschau, 53, pp.298~308 (2006)
  11. Borenslein, B, "Carotenoids, properties and utilization in food", Advance in food Research, 15, pp.195~ (1996)
  12. Bruce, L, "Heat treatments increase sweetness and flesh colour of buttercup squash", New Zealand J. of Crop and Hort. Sci, 27, pp.265~271 (1999)
  13. Chavasit, V, "Changes in β-carotene and vitamin A content of vitamin A-rich foods in Thailand during preservation and storage", J. of Food Sci, 67, pp.375~379 (2002)
  14. Chung, HD, "Ecological and morphological characteristics of Korean native Squash (Cucurbit mosachata)", J. Kor. Soc. Hort. Sci, 39, pp.377~384 (1998)
  15. Commission International de L’Eclairage (CIE), "Recommendations on uniform color spaces-color-difference equations, psychometric color terms, Publ. 15, Suppl. 2" (1976), CIE
  16. D’Souza, MC, "Lycopene concentration of tomato fruit can be estimated from chromaticity values", HortScience, 27, pp.465~466 (1992)
  17. Di Mascio, P, "Lycopene as the most efficient biological carotenoid singlet oxygen quancher", Arch. Biochem. Biophys, 274, pp.532~538 (1989)
  18. Duncan, DB, "Multiple range and multiple F-test", Biometrics, 11, pp.1~42 (1955)
  19. Emenhiser, C, "Capability of polymeric C30 stationary phase to resolve cis-trans carotenoid isomers in reversed-phase liquid chromatography", J. Chromatogr, 707, pp.205~216 (1995)
  20. "Crop production stats" (2008)[URL]
  21. ias, FJ, "Relationship between flesh color and pigment content in squash", Amer. Soc. Hort. Sci, 81, pp.408~414 (1962)
  22. Fraser, PD, "Carotenoid biosynthesis during tomato fruit development", Plant Physiol, 105, pp.405~413 (1994)
  23. Gajc-Wolska, J, "The fruit quality assessment of the chosen hybrids and cultivars of pumpkin (Cucurbita maxiam Duch)", Vegetable Crops Res. Bull, 63, pp.33~43 (2005)
  24. Gross, J, "Pigments in vegetables: Chlorophylls and carotenoids" (1991), Van Nostrand Reinhold
  25. Ha, JL, "Quantitative analysis of carotenoids in Carrot cultivars produced in Korea", J. of the Envi. Sci, 18, pp.1135~1141 (2009)
  26. Halliwell B, "Free Radicals in Biology and Medicine. Third editions" (1999), Oxford University Press
  27. Hidaka, T, "The composition and vitamin A value of the carotenoids of pumpkins of different colors", J. Food Biochemistry, 11, pp.59~68 (1987)
  28. Jeffrey, C, "Systematic of the Cucurbitaceae: an overview, In Biology and utilization of the Cucurbitaceae", pp.3~9 (1990), Cornell University Press
  29. Kato, M, "Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit", Plant Physiol, 134, pp.824~837 (2004)
  30. 김동석, "조리 방법에 따른 호박 중의 클로로필과 카로틴 함량 변화", 동아시아식생활학회지, 14(6), pp.618~624 (2004), 동아시아식생활학회
  31. Kim, JB, "Biological activities and analysis of Carotenoids in plants", Korean J. Crop Sci, 48, pp.72~78 (2003)
  32. 김정순, "Agronomic Characteristics of Squash Germplasm with a low viability in National Agrobiodiversity Center, Korea", 한국작물학회지, 55(1), pp.47~57 (2010), 한국작물학회
  33. 김성란, "단호박과 늙은 호박의 영양성분 및 항산화활성 비교", 한국식품과학회지, 37(2), pp.171~177 (2005), 한국식품과학회
  34. Konopacka, D, "Studies on the usefulness of Cucurbita maxima for the production of ready to eat dried vegetable snacks with a high carotenoid content", LWT- Food Sci. and Tech, 43, pp.302~309 (2010)
  35. Lee, CY, "Carotenoids and vitamin A value of flesh and canned squashes", Nutrition Reports International, 29, pp.129~ (1984)
  36. Lee, HD, "Studies on the quality evaluation of Korean red pepper by color measurement", Kor. J. Dietary Culture, 7, pp.105~112 (1992)
  37. Murkovic, M, "Development of an Austrian carotenoid database", J. Food Comp. Anal, 13, pp.435~440 (2000)
  38. Murkovic, M, "Carotenoid content in different vatietis of pumpkins", J. of Food Composition and Analysis, 15, pp.633~638 (2002)
  39. Nakkanong, K, "Carotenoid accumulation and carotenogenic in gene expression during fruit development in novel interspecific inbred Squash lines and their parents", J. Agric. Chem, 60, pp.5936~5944
  40. Nishino, H, "Cancer prevention by carotenoids", Matat. Res, 402, pp.159~ (1998)
  41. "Dietary supplement fact sheet: Vitamin A and carotenoids" (2006)[URL]
  42. Olson, JA, "The colorful, fascinating world of the carotenoids: important physiologic modulators", FASEB J, 9, pp.1547~1550 (1995)
  43. Paris, HS, "History of the cultivar-groups of Cucurbita pepo", Hort. Rev, 25, pp.71~170 (2001)
  44. Paris, HS, "The genes of pumpkin and squash", Hortscience, 40, pp.1620~1630 (2005)
  45. Park YK, "Changes of carotenoids of the Pumpkin powder during storage", J. Korean Soc. Food Sci. Nutr, 26, pp.32~36 (1997)
  46. Park, SW, "Quality evaluation by color determination in Carrot (Daucus carota L.) -Estimation of total carotenoid content by Colorimeter", J. Kor. Soc. Hort. Sci, 36, pp.481~485 (1995)
  47. Park, YK, "Changes of carotenoids of the pumpkin powder during storage", J. Korean Soc. Food Sci. Nutr, 26, pp.32~36 (1997)
  48. Pedrosa, JF, "Changes in composition of squashes and pumpkin during storage", Res. Agr. Braz, 18, pp.29~32 (1983)
  49. Peirce, LC, "Vegetables Chacateristics, Production, and Marketing", pp.374~380 (1987), John Wiley & Sons Inc.
  50. Rachel, A, "Correlation between L* a* b* color space values and carotenoid content in pumpkins and squash (Cucurbita spp.)", HortScience, 44, pp.633~637 (2009)
  51. Ramos, DRM, "Determination of the vitamin A value of common Brazilian leafy vegetables", J. Micronutr. Anal, 3, pp.147~155 (1987)
  52. Reeves, M.J., "Re-evlauation of Capsicum color data", J. Food Sci, 52, pp.1047~1049 (1987)
  53. Robinson, RW, "Cucurbits" (1997), CAB International
  54. STAT User’s Guide: Version 8.2, "SAS", pp.2002, SAS Institute
  55. Seroczynska, A, "Relationship between carotenoid content and flower or fruit flesh colour of winter squash (Cucurbita maxiam Duch)", Horticulturae, 18, pp.51~61 (2006)
  56. Simonme, AH, "Assessment of beta-carotene content in sweet potato (Ipomoea batatas Lam.) breeding lines in relation to dietary requirements", J. Food Compost. Anal, 6, pp.336~345 (1993)
  57. Sztangret, J, "Assessment of yield, dry matter and carotenoids content in the new hybids of winter squash (Cucurbita maxima Duch.)", Folia Hortculturae Ann, 13, pp.437~443 (2001)
  58. Tadmor, Y, "Dual role of the pigmentation gene B in affecting carotenoid and vitamin E content in squash (Cucurbitapepo) mesocarp", J. Agr. Food Chem, 53, pp.9759~9763 (2005)
  59. Takeda, K, "Chemistry of flower pigments: a review", Kagaku-no Ryoiki, 36, pp.69~ (1982)
  60. Thomas, CA, "A Preliminary Survey of Oilseeds in the Cucurbitaceae", Cucurbit Genetics Cooperative Report, 23, pp.96~98 (2000)
  61. Whang, HJ, "Carotenoid pigment of Pumpkin cultivated in Korea", Korean J. Food & Nutr, 12, pp.508~512 (1999)
  62. Wills, RB, "Composition of Australian foods. Vegetable Fruits", Food Technology in Australia, 39, pp.388~ (1987)

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