Microplate법에 의한 수박 라이코핀 고함유 유전자원 선발
Screening of Lycopene-rich Germplasms using Microplate Method in Watermelon (Citrullus Lanatus (thunb.) Matsum. & Nakai)
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초록
Recent researches focused on breeding lycopene–rich watermelon varieties are active since lycopene content inwatermelon is 60% higher than in tomato. We developed a new microplate method using a UV spectrophotometer to selectgenetic resources of watermelon containing high lycopene content. The microplate method showed more accurate analysis (about97% positive correlation) than HPTLC, suggesting the microplate method is a reliable analytical technique for the rapid andsimple selection of watermelon resources. Lycopene contents of 96 watermelon accessions using the new microplate method wasa wide variation of 3.2-98.3 ㎍.g-1FW to assess, allowing selection of 15 high-lycopene watermelon accessions. Lycopene contentsof the 15 watermelon accessions was 4.4∼9.6 fold higher than lycopene content of a commercial cultivar ‘Speedplus’. Theseresults suggest that the watermelon accessions selected using a novel microplate method can be used for breeding of lycopene-richcultivars.
Abstract
Recent researches focused on breeding lycopene–rich watermelon varieties are active since lycopene content inwatermelon is 60% higher than in tomato. We developed a new microplate method using a UV spectrophotometer to selectgenetic resources of watermelon containing high lycopene content. The microplate method showed more accurate analysis (about97% positive correlation) than HPTLC, suggesting the microplate method is a reliable analytical technique for the rapid andsimple selection of watermelon resources. Lycopene contents of 96 watermelon accessions using the new microplate method wasa wide variation of 3.2-98.3 ㎍.g-1FW to assess, allowing selection of 15 high-lycopene watermelon accessions. Lycopene contentsof the 15 watermelon accessions was 4.4∼9.6 fold higher than lycopene content of a commercial cultivar ‘Speedplus’. Theseresults suggest that the watermelon accessions selected using a novel microplate method can be used for breeding of lycopene-richcultivars.
참고문헌 (17)
- Andola HC, "High Performance Thin Layer Chromatography(HPTLC) : A modern analytical tool for biological analysis", Nature & Science, 8, pp.58~61 (2010)
- Arias R, "Correlation of lycopene measured by HPLC with the L*, a*, b* color readings of a hydroponic tomato and the relationship of maturity with color and lycopene content", J. Agric. Food Chem, 48, pp.1697~1702 (2000)
- Di Mascio P, "Lycopene as the most effective biological carotenoid singlet oxygen quencher", Arch. Biochem. Biophys, 274, pp.532~538 (1989)
- Fish, WW, "The effects of frozen storage conditions on lycopene stability in watermelon tissue", J. Agric. Food Chem, 51, pp.3582~3585 (2003)
- Gerster H., "The potential role of lycopene for human health", J. Am. Coll. Nutr, 16, pp.109~126 (1997)
- Giovannucci E, "Intake of carotenoids and retinol in relation to risk of prostate cancer", J. Natl. Cancer 1nst, 87, pp.1767~1776 (1995)
- Giovannucci E., "Tomatoes. tomato-based products, lycopene, and cancer: review of the epidemiological literature", J. Natl. Cancer 1nst, 91, pp.317~331 (1999)
- Gerster H., "The potential role of lycopene for human health", J. Am. Coll. Nutr, 16, pp.109~126 (1997)
- Hasler CM, "Functional foods: their role in disease prevention and health promotion", Food Technol, 52, pp.63~69 (1998)
- Olives Barbaa AI, "Application of a UV–vis detection-HPLC method for a rapid determination of lycopene and β-carotene in vegetables", Food Chem, 95, pp.328~336 (2006)
- Perkins-Veazie P, "Lycopene content differs among red-fleshed watermelon cultivars", J. Sci. Food Agric, 81, pp.983~987 (2001)
- Rao AV, "Bioavailability and in vitro antioxidant properties of lycopene from tomato products and their possible role in the prevention of cancer", Nutr. Cancer, 31, pp.199~203 (1998)
- Stahl W, "Lycopene: A biologically important carotenoid for humans?", Arch. Biochem. Biophys, 336, pp.1~9 (1996)
- Tee ES, "Carotenoid composition and content of Malaysian vegetables and fruits by the AOAC and HPLC methods", Food Chem, 41, pp.309~339 (1991)
- Vasta JD, "Analysis of lycopene in nutritional supplements by silica gel high-performance thin-layer chromatography with visible-mode densitometry", Acta Chromatographica, 20, pp.673~683 (2008)
- 김수, "고온에 안정적인 고추 TSWV(Tomato spotted wilt virus) 저항성 유전자원 탐색", 한국육종학회지, 44(4), pp.559~566 (2012), 한국육종학회[DOI]