무선발 형질전환 Bt 벼 현미의 주요 영양성분 분석
Comparative Nutritional Analysis for Marker-Free Transgenic Bt rice and Non-Transgenic Counterparts
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
본 연구는 Bacillus thuringiensis 유래의 살충성 mCry1Ac 유전자를 무선발 형질전환 방법으로 일미 벼에 도입하여 개발된 마커프리 형질전환 Bt 벼 2계통의 일반성분 및 주요성분(무기질, 아미노산) 함량을 확인하여 모본벼 및 다른 일반품종과 함량차이를 비교 분석 함으로서 형질전환 벼의 영양성분 동등성 여부를 확인하고자 수행되었다. 영양성분 분석결과 GM 벼 현미의 일반성분 조성 중 식이섬유 함량과 일부 무기질 함량이 모본 벼인 일미와 비교하여 다소 유의적 차이가 있었지만 일반품종에서 나타나는 함량범위 안에 포함되는 수치이며, 아미노산 성분과 대부분의 일반성분 및 무기질의 함량은 전반적으로 모본과 유의적 차이가 없었다. 따라서 형질전환 벼에서 관찰된 일부 성분 차이는 Bt 유전자의 도입 효과가 아닌 재배 환경 및 토양성분의 차이에서 기인된 것으로 형질전환에 의한 비의도적 영양성분 변화는 없는 것으로 판단된다.
Abstract
The selectable marker-free rice plants containing mcry1Ac insecticidal gene isolated from Bacillus thuringiensis (Bt)were generated using a non-selection approach by Agrobacterium tumefaciens-mediated transformation. The nutritional compositionof two lines of transgenic rice plants (RTB5 and RTB11) was compared with that of its non-transgenic counterpart. The resultsshowed that, except for small differences in dietary fiber and some minerals, there was no significant difference between transgenicrice and conventional counterpart variety with respect to their nutrient composition. Most of measured levels of nutrients werewithin the range of values reported for other commercial cultivars, showing substantial equivalency. Therefore, the insertion oftransgenes did not affect the nutritional composition of transgenic RTB5 and RTB11 rice grains.
참고문헌 (18)
- AOAC, "Official methods of analysis of the Association of Official Analytical Chemists" (1990), Association of Official Analytical Chemists
- Hoon Choi, "Comparative Nutritional Analysis for Genetically Modified Rice, Iksan483 and Milyang204, and Nontransgenic Counterparts", Applied Biological Chemistry, 55(1), pp.19~26 (2012), 한국응용생명화학회
- Damalas CA, "Pesticide exposure, safety issues, and risk assessment indicators", Int. J. Environ. Res. Pub. Health, 8, pp.1402~1419 (2011)
- EFSA, "Scientific opinion on statistical considerations for the safety evaluation of GMOs", EFSA J, 8, pp.1250~1259 (2010)
- Global Status of Commercialized Biotech, "GM Crops: 2013", James C., 2013, ISAAA Brief No. 46
- KFDA, "Korean Food Standards Codex", Korea Food & Drug Administration, ~ (2011)
- Kim EH, "Chloroplast-targeted expression of synthetic cry1Ac in transgenic rice as an alternative strategy for increased pest protection", Planta, 23, pp.397~405 (2009)
- 이근표, "형질전환작물의 위해성평가와 품종등록", Journal of Plant Biotechnology, 35(1), pp.13~21 (2008), 한국식물생명공학회
- 이소영, "형질전환 해충저항성 벼의 주요영양성분 및 항영양소 분석", 한국육종학회지, 46(4), pp.400~407 (2014), 한국육종학회[DOI]
- 이영택, "유전자변형 베타-카로틴 강화 쌀의 주요 영양성분 분석", 한국식품영양과학회지, 39(1), pp.105~109 (2010), 한국식품영양과학회
- Oberdoerfer RB, "Rice (Oryza sativa L.) containing the bar gene is compositionally equivalent to the nontransgenic counterpart", J. Agri. Food Chem, 53, pp.1457~1465 (2005)
- OECD, "Series on the safety of novel food and feed No. 10. consensus document on compositional considerations for new varieties of rice (Oryza sativa): key food and feed nutrients and anti-nutrients", ~ (2004)
- 박수윤, "Comparison of the Nutritional Compositions of Insect-Resistant and Glufosinate-Tolerant Rice and Conventional Rice", Journal of Applied Biological Chemistry, 56(1), pp.5~9 (2013), 한국응용생명화학회
- Song BH, "Distribution of amino acids and fatty acids within the degermed brown rice kernel", J. Korean Agric. Chem. Soc, 31, pp.7~12 (1988)
- 우희종, "국내외 상업화 GM 작물의 유전요소 분석", Journal of Plant Biotechnology, 36(2), pp.106~114 (2009), 한국식물생명공학회
- 우희종, "무선발 형질전환 Bt벼의 도입유전자 구조 분석", Journal of Plant Biotechnology, 40(3), pp.135~140 (2013), 한국식물생명공학회
- Hee-Jong Woo, "Strategies for Developing Marker-free Transgenic Plants", Biotechnology and Bioprocess Engineering, 16(6), pp.1053~1064 (2011), 한국생물공학회
- Xin L, "Comparative analysis of nutritional composition between herbicide-tolerant rice with bar gene and its non-transgenic counterpart", J. Food Compos. Anal, 21, pp.535~539 (2008)