제초제 저항성 Ab벼와 해충저항성 Bt벼의 성분분석
Composition Analysis of Herbicide Tolerant Ab Rice and Insect-Resistant Bt Rice
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초록
본 연구에서는 제초제 저항성인 Ab벼와 해충저항성 Bt벼, 이들의 모본인 동진벼의 현미에서 일반성분, 아미노산, 무기물, 지방산, 비타민 및 항영양소를 분석하여 유전자변형 벼(Ab, Bt)의 영양성분 조성 차이를 비교하고자 수행하였다. 그결과 무기물의 철분이 모본인 동진벼와 유전자 변형 벼(Ab, Bt) 사이에 약 2배의 차이를 나타내었지만 codex 범위 내에존재하였고, 비타민 류에서는 Bt벼와 동진벼 간의 유의적인차이가 나타나지 않은 반면 Ab벼의 비타민 B1, B7, E에서 유의적인 차이를 보였다. 또한 트립신 저해제는 시료 모두에서0.1 TIU/mg 미만으로 극소량 검출되었다. 총 46가지 분석 성분 중 17가지가 함량 및 조성에 유의적인 차이를 보였으나 이러한 차이는 이들의 모본인 동진벼, OECD 표준기술서, 그리고 일반 상업화 품종의 범위 내에 존재해 영양학적 측면에서실질적으로 차이가 없는 것으로 판단된다.
Abstract
In order to assess the substantial equivalence of two varieties of genetically modified rice, herbicide-tolerant Ab rice and the insect-resistant Bt rice, to the non-GM Dongjin-byeo cultivar. We analyzed the compositions and contents of the proximate, amino acids, minerals, fatty acids, vitamins, and anti-nutrients in their unpolished grains using t-test (p<0.05). A comparison of fatty acids compositions showed that the levels of stearic acid and arachidonic acid in Ab rice and those of myristic, palmitic, oleic, linoleic, and gadoleic acid in Bt rice were different significantly from the corresponding levels in Dongjin-byeo. Vitamin content did not differ between Bt and Dongjin-byeo, but the content of vitamins B1, B7, and E in Ab rice differed from that in Dongjin-byeo. Iron content in Ab and Bt rice was 2 times higher than that in Dongjin-byeo, although it was within the reference range set by Codex. The amount of the anti-nutrient trypsin inhibitor was 0.1 TIU/mg in the unpolished grain of all three rice varieties examined. Of the 47 components analyzed, 17 were significantly different among the three rice varieties; however, most of these differences were within the Codex reference range for commercial rice. Overall, it was confirmed that both Bt and Ab rice are substantially equivalent to the Dongjin-byeo and other commercial varieties of rice.
참고문헌 (35)
- AOAC, "Fat (Crude) or ether extract in meat. AOAC official methods 960.39: Gaithersburg", ~ (2000)
- AOAC, "Determination of lead, calcium, copper, iron, zinc, sodium, magnesium, phosphorus, potassium, manganese in food. AOAC official methods 999.11:Gaithersburg", ~ (2000)
- AOAC, "Ash of flour. AOAC official methods 923.03: Gaithersburg", ~ (2005)
- AOAC, "Nitrogen (total) in fertilizers. AOAC Official methods 955.04: Gaithersburg", ~ (2005)
- AOAC, "Protein efficiency ratio. AOAC official methods 982.30: Gaithersburg", ~ (2005)
- AOCS, "In official methods and recommended practices of the American oil chemists. Official method Ba 12-75. Champaign", ~
- AOCS, "Fatty acid composition by gas chromatography. In official methods and recommended practices of the American oil Chemists. Method Ce 1-62. Champaign", 1~62 (1997)
- Betz FS, "Safety and advantages of Bacillus thuringiensis-protected plants to control insect pests", Regul. Toxicol. Pharmacol, 32, pp.156~173 (2000)
- Cho HS, "Development of “basta” resistant Chinese cabbage by transfer of bar gene", Korean J. Breed. Sci, 33, pp.324~331 (2001)
- 최정숙, "쌀 품종별 백미와 현미의 영양성분 조성 비교", 한국식품영양과학회지, 31(5), pp.885~892 (2002), 한국식품영양과학회
- Crapo, PA, "Postprandial plasma-glucose and –insulin responses to different complex carbohydrate", Diabetes, 26, pp.1178~ (1977)
- Eliseu S, "Transformation of potato (Solanum tuberosum) cv. Mantiqueira using Agrobacterium tumefaciens and evaluation of herbicide resistance", Plant Cell Rep, 13, pp.666~670 (1994)
- Graham B, "Global impact of biotech crops: income and production effects, 1996-2007", AgBioForum, 12, pp.184~208 (2009)
- HeinemannRJB, "Comparative study of nutrient composition of commercial brown, parboiled and milled rice from Brazil", J. Food Comp. Anal, 18, pp.287~296 (2005)
- Korea Food and Drug Administration, "Food Standard Code. Korea Food & Drug Administration", ~ (2011)
- 김형진, "유전자변형 생물체의 위해성평가", 한국독성학회지, 19(1), pp.1~12 (2003), 한국독성학회
- Kim JK, "Determination of lipophilic compounds in genetically modified rice using gas chromatography-time-of-flight mass spectrometry", J. Food Comp. Anal, 25, pp.31~38 (2012)
- Kim JK, "Stable isotope dilution gas chromatography-time-of-flight mass spectrometry method for determining niacin in rice", Cereal Chem, 88, pp.397~399 (2011)
- 김재광, "Unintended polar metabolite profiling of carotenoid-biofortified transgenic rice reveals substantial equivalence to its non-transgenic counterpart", Plant Biotechnology Reports, 7(1), pp.121~128 (2013), 한국식물생명공학회
- 권오윤, "국내 주요 쌀 품종별 영양성분 및 주요 항영양인자 분석", 한국식품조리과학회지, 22(6), pp.949~956 (2006), 한국식품조리과학회
- 이지현, "조리방법에 따른 가금류의 부위별 지용성 영양성분 함량 변화 조사: 콜레스테롤, 레티놀 및 알파-토코페롤", 한국식품영양과학회지, 44(2), pp.234~241 (2015), 한국식품영양과학회
- 이소영, "형질전환 해충저항성 벼의 주요영양성분 및 항영양소 분석", 한국육종학회지, 46(4), pp.400~407 (2014), 한국육종학회[DOI]
- 이영택, "형질전환 가뭄저항성 벼 현미의 주요 영양성분 분석", 한국식품영양과학회지, 42(5), pp.730~735 (2013), 한국식품영양과학회
- Liu Z, "Grain phytic acid content in japonica rice as affected by cultivar and environment and its relation to protein content", Food Chem, 89, pp.49~52 (2005)
- Mendelson M, "Are Bt safe?", Nat. Biotechnol, 21, pp.1003~1009 (2003)
- Nehra NS, "Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs", Plant J, 5, pp.285~297 (1994)
- OECD, "Environment, health and safety publications series on the safety of novel foods and feeds. No. 10. Consensus document on compositional considerations for new carieties of rice (Oryza sativa): Key food and feed nutrients and anti-nutrients", ~ (2004)
- Parengam M, "Study of nutrients and toxic minerals in rice and legumes by instrumental neutron activation analysis and graphite furnace atomic absorption spectrophotometer", J. Food Comp. Anal, 23, pp.340~345 (2010)
- 박수윤, "Comparative Analysis of Nutritional Composition between the Disease-resistant Rice Variety OsCK1 and Conventional Comparators", Food Science and Biotechnology, 24(1), pp.225~231 (2015), 한국식품과학회[DOI]
- Soo-Yun Park, "Compositional comparative analysis between insect-resistant rice (Oryza sativa L.) with a synthetic cry1Ac gene and its non-transgenic counterpart", Plant Biotechnology Reports, 6(1), pp.29~37 (2012), 한국식물생명공학회[DOI]
- Schnepf HE, "Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli", Proc. Natl. Acad. Sci. USA, 78, pp.2893~2897 (1981)
- Son SM, "Rice based meal for prevention of obesity and chronic disease", Korean J. Community Nutr, 6, pp.862~867 (2001)
- 원용재, "형질전환 벼 이용 품종 조기개발 체계 확립", Journal of Plant Biotechnology, 31(1), pp.7~11 (2004), 한국식물생명공학회
- 우희종, "Comparison of the nutritional compositions of oxidative stress-tolerant transgenic rice and conventional rice", Journal of Plant Biotechnology, 41(4), pp.206~211 (2014), 한국식물생명공학회