93-11 × 밀양352 약배양 집단을 이용한 벼 출수기 QTL분석
QTL Analysis of Heading Date Using 93-11 × Milyang352 Doubled Haploid Lines in Rice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Rice doubled haploid (DH) lines derived from crosses of the indica cultivar 93-11 and japonica line Milyang352 were used in genetic mapping and QTL analysis studies of days to heading (DTH), an important trait that controls rice yield and biomass. QTL mapping was conducted using the inclusive composite interval mapping method. We used 234 single-nucleotide polymorphic markers in the whole-genome region, including 100 KASP markers and 134 Fluidigm markers, to construct a genetic map. DH populations were raised in Milyang, Korea, over three different periods. Two major DTH QTLs, qDTH3-1 and qDTH7, were detected under natural conditions in Milyang, and explained 14.88%~24.56% and 24.20%~37.39% of the phenotypic variation, respectively, in 93-11 ×Milyang352 DH populations. During three different rice cultivation periods, qDTH3-1 and qDTH7 were repeatedly detected with significant logarithm of the odds scores and phenotypic variability explained. The findings of this study will make a valuable contribution to breeding high-yielding and early-maturing rice in Korea.
Abstract
Rice doubled haploid (DH) lines derived from crosses of the indica cultivar 93-11 and japonica line Milyang352 were used in genetic mapping and QTL analysis studies of days to heading (DTH), an important trait that controls rice yield and biomass. QTL mapping was conducted using the inclusive composite interval mapping method. We used 234 single-nucleotide polymorphic markers in the whole-genome region, including 100 KASP markers and 134 Fluidigm markers, to construct a genetic map. DH populations were raised in Milyang, Korea, over three different periods. Two major DTH QTLs, qDTH3-1 and qDTH7, were detected under natural conditions in Milyang, and explained 14.88%~24.56% and 24.20%~37.39% of the phenotypic variation, respectively, in 93-11 ×Milyang352 DH populations. During three different rice cultivation periods, qDTH3-1 and qDTH7 were repeatedly detected with significant logarithm of the odds scores and phenotypic variability explained. The findings of this study will make a valuable contribution to breeding high-yielding and early-maturing rice in Korea.
참고문헌 (31)
- 천경성, "Single Nucleotide Polymorphism (SNP) Discovery and Kompetitive Allele-Specific PCR (KASP) Marker Development with Korean Japonica Rice Varieties", Plant Breeding and Biotechnology, 6(4), pp.391~403 (2018), 한국육종학회[DOI]
- Doi K, "Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1", Genes Dev, 18, pp.926~936 (2004)
- Dong Y, "Mapping of QTLs for leaf developmental behavior in rice(Oryza sativa L. )", Euphytica, 138, pp.169~175 (2004)
- Fujino K, "A novel genotype DATTO5developed using the five genes exhibits the fastest heading date designed in rice", Korean J Breed Sci, 70, pp.193~199 (2020)
- Gao H, "Days to heading 7, a major quantitative locus determining photoperiod sensitivity and regional adaptation in rice", Proc Natl Acad Sci USA, 111, pp.16337~16342 (2014)
- Hittalmani S, "Molecular mapping of quantitative trait loci for plant growth, yield and yield related traits across three diverse locations in a doubled haploid rice population", Euphytica, 125, pp.207~214 (2002)
- Hori K, "Hd16, a gene for casein kinase I, is involved in the control of rice flowering time by modulating the day-length response", Plant J, 76, pp.36~46 (2013)
- 지현소, "벼의 밀양23호", 기호벼 재조합자식 유전집단을 이용한 PCR 기반 DNA 마커들로 구성된 분자유전자지도 작성, 2012, pp.44(3), 273~281[DOI]
- Kabange NR, "Indentification of a novel QTL for chlorate resistance rice(Oryza sativa L)", Agriculture, 10, pp.1~16 (2020)
- Komiya R, "Hd3a and RFT1 are essential for flowering in rice", Development, 135, pp.767~774 (2008)
- Kwon CT, "Natural variation in Early flowering1 contributes to early flowering in japonica rice under long days", Plant Cell Environ, 37, pp.101~112 (2014)
- Lee BS, "A Study of Natural Seasons in Korea", J Geol Soc, 14, pp.1~11 (1979)
- 이소명, "QTL Analysis for Fe and Zn Concentrations in Rice Grains Using a Doubled Haploid Population Derived from a Cross Between Rice (Oryza sativa) Cultivar 93-11 and Milyang 352", Plant Breeding and Biotechnology, 8(1), pp.69~76 (2020), 한국육종학회[DOI]
- Lin HX, "RFLP mapping of major genes and minor genes for heading date in rice", Rice Genet Newsl, 12, pp.253~255 (1995)
- Lin S, "Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines", Theor Appl Genet, 96, pp.997~1003 (1998)
- Maheswaran M, "Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population", Theor Appl Genet, 94, pp.39~45 (1997)
- Meng L, "QTL IciMapping : integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations", Crop J, 3, pp.269~283 (2015)
- Mo Y, "Characterization of QTLs and candidate genes for days to heading in rice recombinant inbred lines", Genes, 11, pp.562~ (2020)
- Murakami M, "The evolutionarily conserved OsPRR quintet : rice pseudo-response regulators implicated in circadian rhythm", Plant Cell Physiol, 44, pp.1229~1236 (2003)
- Murray M, "Rapid isolation of high molecular weight plant DNA", Nucleic Acids Res Spec Publ, 8, pp.4321~4326 (1980)
- Seo J, "Characterization of the common Japonica-originated genomic regions in the high-yielding varieties developed from inter-subspecific crosses in temperate rice(Oryza sativa L. )", Genes, 11, pp.562~ (2020)
- Takahashi Y, "Hd6, a rice quantitative trait locus involved in photoperiod sensitivity, encodes the α subunit of protein kinase CK2", Proc Natl Acad Sci USA, 98, pp.7922~7927 (2001)
- Takeuchi Y, "Developing isogenic lines of Japanese rice cultivar ‘Koshihikari’ with early and late heading", Jpn Agric Res Q, 45, pp.15~22 (2011)
- Wang P, "Improving rice yield and quality by QTL pyramiding", Mol Breed, 29, pp.903~913 (2012)
- Wei X, "DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously", Plant Physiol, 153, pp.1747~1758 (2010)
- Xiao J, "Identification of QTLs affecting traits of agronomic importance in a recombinant inbred population derived from a subspecific rice cross", Theor Appl Genet, 92, pp.230~244 (1996)
- Xue W, "Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice", Nat Genet, 40, pp.761~767 (2008)
- Yamamoto T, "Fine mapping of quantitative trait loci Hd-1, Hd-2 and Hd-3, controlling heading date of rice, as single Mendelian factors", Theor Appl Genet, 97, pp.37~44 (1998)
- Yang G, "Development of a core SNP arrays based on the KASP method for molecular breeding of rice", Rice, 12, pp.21~ (2019)
- Yang Z, "Analysis of epistasis among QTLs on heading date based on single segment substitution lines in rice", Sci Rep, 8, pp.1~8 (2018)
- Yano M, "Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS", Plant Cell, 12, pp.2473~2483 (2000)