호밀 염색체 도입 밀 계통 선발을 위한 RAPD, GISH 및 FISH분석

Abstract

Rye (Secale cereale L.) is known as an important source of useful genes for the improvement of resistance to rust, powdery mildew and tolerance to drought and cold on wheat (Triticum aestivum L.). We describe the identification and utilization of rye genome- specific DNA markers, OPH20-1494 bp and OPC10-1012 bp, by RAPD analysis. These markers were used to detect rye chromatin among 467 BC1F6 lines derived from the backcross of the F1 hybrid of wheat cv. Olmil and rye cv. Paldanghomill. Thirty-two lines out of 467 BC1F6 population possessed the part of rye genome. Moreover GISH and FISH analysis confirmed the presence of rye chromosome fragment on wheat background in these lines. Therefore these rye-specific markers can be used effectively in the breeding program for introgression of rye chromatin into wheat.