호밀(Secale cereale L.)의 핵형분석과 rDNA의 Physical Mapping
Karyotype Analysis and rDNA Physical Mapping in Rye (Secale cereale L.)
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참고문헌
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초록
지닌 호밀을 Gimsa C-분염법과 FISH기법을 이용하여 구성이질 염색질과 5S와 18S-26S rRNA 유전자의 염색체상의위치를 확인하고자 본 실험을 수행하였다. 그 결과를 요약하면 아래와 같다. 1. C-분염의 분석 결과 7쌍의 호밀의 염색체 중에서 1, 2,3과 7번 염색체는 중부염색체 이었고, 4, 5과 6번 염색체는차중부염색체 이었다. 1번 염색체는 2차 협착부위로 동원체를 지니고 있었다. 2. FISH 분석에서 5S rDNA 유전자는 4개의 염색체에서표지되었으며 2차협착으로 부수체가 존재하는 1번 염색체의부수체의 말단과 5번 염색체의 중간에 표지되었고, 18S-26S rDNAs 유전자는 1개의 염색체에 표지되었으며 이 염색체는2차협착으로 부수체가 존재하는 1번 염색체의 인 형성체 부위에 표지되었다. 1번 염색체에는 5S 와 18S-26S rDNAs 유전자가 표지되었고 5번 염섹체에는 5S rDNA 유전자만이 표지되었다.
Abstract
This study was carried out to determine the chromosomal localization of the 5S and 18S-26S ribosomal DNA(rDNA)genes by means of fluorescence in situ hybridization(FISH) techniques, and the constitutive heterochromatin detected by means of Gimsa C-banding technique in rye(Secale cereale L.). The somatic chromosomes number was 2n=14. The karyotype consists of four pairs of metacentrics(chromosomes 1, 2, 3, and 7) and three pairs of submetacentrics(chromosomes 4, 5, and 6). Secondary constrictions appeared in the short arm of chromosome 1. The 5S rDNA genes have been located on two pairs of chromosomes 1 and 5, and 18S-26S rDNAs genes have been located on one pair of chromosome 1. 5S rDNA genes were detected on the distal region of the secondary constrictions in nucleolus organizer regions(NOR) in chromosome 1, and other detected on the intercalary region in the short arm of chromosome 5.
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