SSR 분자마커를 이용한 색소옥수수 계통들에 대한 계통유연관계 및 집단구조 분석
Analysis of the Genetic Relationship and Population Structure for Colored Maize Maize Lines Using SSR Markers
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
In this study, genetic diversity, genetic relationships, and population structure were investigated among colored maize lines (12 colored normal maize lines and 12 colored waxy maize lines) and non-colored maize lines (2 normal maize inbred lines and 5 waxy maize inbred lines) using 50 SSR markers. A total of 282 alleles were identified at all the loci with an average of 4.84 and a range between 2 and 11 alleles per locus. The major allele frequency varied from 0.23 to 0.90 with an average of 0.45. The genetic diversity values varied from 0.17 to 0.86 with an average of 0.67. To evaluate the population structure,STRUCTURE 2.2 program was employed to confirm genetic structure. The 31 colored and non-colored maize lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II, III, IV, V, and admixed group. The cluster tree generated using the described SSR markers recognized three major groups at 27.4% genetic similarity. Group I included 10maize lines (2 normal maize lines, 2 waxy maize lines, 6 colored normal maize lines), and Group II included 20 maize lines (3 waxy maize lines, 5 colored normal maize lines, 12 colored waxy maize lines). Group III consist of only 1 colored normal maize inbred line. The present study has demonstrated the utility of SSR analysis for the study of genetic diversity, genetic relationships, and the population structure among colored and non-colored maize lines.
Abstract
In this study, genetic diversity, genetic relationships, and population structure were investigated among colored maize lines (12 colored normal maize lines and 12 colored waxy maize lines) and non-colored maize lines (2 normal maize inbred lines and 5 waxy maize inbred lines) using 50 SSR markers. A total of 282 alleles were identified at all the loci with an average of 4.84 and a range between 2 and 11 alleles per locus. The major allele frequency varied from 0.23 to 0.90 with an average of 0.45. The genetic diversity values varied from 0.17 to 0.86 with an average of 0.67. To evaluate the population structure,STRUCTURE 2.2 program was employed to confirm genetic structure. The 31 colored and non-colored maize lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II, III, IV, V, and admixed group. The cluster tree generated using the described SSR markers recognized three major groups at 27.4% genetic similarity. Group I included 10maize lines (2 normal maize lines, 2 waxy maize lines, 6 colored normal maize lines), and Group II included 20 maize lines (3 waxy maize lines, 5 colored normal maize lines, 12 colored waxy maize lines). Group III consist of only 1 colored normal maize inbred line. The present study has demonstrated the utility of SSR analysis for the study of genetic diversity, genetic relationships, and the population structure among colored and non-colored maize lines.
참고문헌 (33)
- Akagi H, "Highly polymorphic microsatellites of rice consist of AT repeats, and a classification of closely related cultivars with these microsatellite loci", Theor. Appl. Genet, 94, pp.61~67 (1997)
- Blair MW, "Genetic diversity, seed size association and population structure of a core collection of common beans (phaseolus vulgaris L.)", Theor. Appl. Genet, 119, pp.955~972 (2009)
- Bostein D, "Construction of a genetic linkage map in man using restriction fragment length polymorphisms", Am. J. Genet, 32, pp.314~331 (1980)
- Cevallos-Casals BA, "Stability of anthocyanin-based aqueous extracts of Andean purple corn and red-fleshed sweet potato compared to synthetic and natural colorants", Food Chem, 86, pp.69~77 (2004)
- Cömertpay G, "Diversity Assessment of Turkish Maize Landraces Based on Fluorescent Labelled SSR Markers", Plant Mol. Biol. Rep, 30, pp.261~274 (2012)
- D’hoop BB, "Population structure and linkage disequilibrium unraveled in tetraploid potato", Theor. Appl. Genet, 121, pp.1151~1170 (2010)
- Dellaporta SL, "A simple and rapid method for plant DNA preparation. Version II", Plant Mol Biol Rep, 1, pp.19~21 (1983)
- Dice LR, "Measures of the amount of ecologic association between species", Ecology, 26, pp.297~302 (1945)
- Enoki H, "SSR analysis of genetic diversity among maize inbred lines adapted to cold regions of Japan", Theor. Appl. Genet, 104, pp.1270~1277 (2002)
- Evanno G, "Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study", Mol. Ecol, 14, pp.2611~2620 (2005)
- pyridine (PhIP)-associated colorectal carcinogenesis in male F344 rats pretreated with 1,2- dimethylhydrazine, "Cancer Letters", 2001, 17~25
- Hallauer AR, "Corn breeding, In Corn and Corn Improvement. 3rd ed", pp.463~564 (1988), Agron., Monogr
- Van Inghelandt D, "Population and genetic diversity in a commercial maize breeding program assessed with SSR and SNP markers", Theor. Appl. Genet, 120, pp.1289~1299 (2010)
- Jin L, "Genetic diversity and population structure of a rice germplasm for association mapping", Theor. Appl. Genet, 121, pp.475~487 (2010)
- 김민경, "Identification and quantification of anthocyanin pigments in colored rice", Nutrition Research and Practice, 2(1), pp.46~49 (2008), 한국영양학회
- Labate JA, "Molecular and historical aspects of Corn Belt dent diversity", Crop Sci, 43, pp.80~91 (2003)
- Laborda PR, "Tropical maize germplasm: what can we say about its genetic diversity in the light of molecular markers?", Theor. Appl. Genet, 111, pp.1288~1299 (2005)
- Li J, "Purple corn anthocyanins retard diabetesassociated glomerulosclerosis in mesangial cells and db", db mice, 2011, ~
- Li J, "Population structure and genetic diversity in elite sugar beet germplasm investigated with SSR markers", Euphytica, 175, pp.35~42 (2010)
- Liu K, "PowerMarker: an integrated analysis environment for genetic marker analysis", Bioinformatics, 21, pp.2128~2129 (2005)
- Liu KJ, "Genetic structure and diversity among maize inbred lines as inferred from DNA microsatellites", Genetics, 165, pp.2117~2128 (2003)
- Matsuoka Y, "Microsatellites in Zea—variability, patterns of mutations, and use for evolutionary studies", Theor. Appl. Genet, 104, pp.436~450 (2002)
- Pejic I, "Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSR, and AFLPs", Theor. Appl. Genet, 97, pp.1248~1255 (1998)
- Pritchard JK, "Documentation for STRUCTURE software: Version 2", ~ (2003)
- Qi-Lun Y, "Constructing a core collection for maize (Zea mays L.) landrace from Wuling mountain region in China", Agr. Sci. China, 7(12), pp.1423~1432 (2008)
- Reif JC, "Genetic structure and diversity of European flint maize populations determined with SSR analysis of individual and bulks", Theor. Appl. Genet, 111, pp.906~913 (2005)
- Rohlf FJ, "NTSYS- pc: Numerical taxonomy and multivariate analysis system. Version: 2.02" (1998), Exeter Software
- Senior ML, "Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system", Crop Sci, 38, pp.1088~1098 (1998)
- Smith JSC, "An evaluation of the utility of SSR loci as molecular marker in maize (Zea mays L.): comparison with RFLPs and pedigree", Theor. Appl. Genet, 95, pp.163~173 (1997)
- Stich B, "Linkage disequilibrium in European elite maize germplasm investigated with SSRs", Theor. Appl. Genet, 111, pp.723~730 (2005)
- Wang ML, "Genetic diversity and population structure analysis of accessions in the US historic sweet sorghum collection", Theor. Appl. Genet, 120, pp.13~23 (2009)
- Wang R, "Population structure and linkage disequilibrium of a mini core set of maize inbred lines in China", Theor. Appl. Genet, 117, pp.1141~1153 (2008)
- Yoshimoto M, "Antimutagenicity of Deacylated Anthocyanins in Purple-fleshed Sweetpotato", Biosci. Biotechnol. Biochem, 65(7), pp.1652~1655 (2001)