SSR 분자마커를 이용한 튀김옥수수 자식계통 집단의 구조 및 association 분석
Analysis of Population Structure and Association Mapping among Popcorn Inbred Lines using SSR markers
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초록
In this study, genetic diversity and population structure among 79 popcorn inbred lines were investigated using 10morphological traits and 50 simple sequence repeat (SSR) markers. From population structure analysis, the 79 maize inbred lines were divided into five groups: I, II, III, IV, and admixed. Among them, 4 inbred lines were assigned to group I, 17 inbred lines were assigned to group II, and 6 and 22 inbred lines were respectively assigned to groups III and IV. The rest 30 inbred lines were contained in the admixed group. Association mapping analysis for popcorn inbred lines was performed to assess the marker-trait associations between the 50 SSR markers and the 10 morphological traits. At a significance level of 0.01, we found 92 marker-trait associations using the Q GLM model. While, using the Q+K MLM model, we found 6 marker-trait associations at a significance level of 0.01. Finally, this study's assessment of population structure and association mapping of 79 popcorn inbred lines will be helpful useful for popcorn breeding programs such activities as planning crosses for hybrid and line development at Maize Experiment Station, Gangwon Agricultural Research and Extension Services.
Abstract
In this study, genetic diversity and population structure among 79 popcorn inbred lines were investigated using 10morphological traits and 50 simple sequence repeat (SSR) markers. From population structure analysis, the 79 maize inbred lines were divided into five groups: I, II, III, IV, and admixed. Among them, 4 inbred lines were assigned to group I, 17 inbred lines were assigned to group II, and 6 and 22 inbred lines were respectively assigned to groups III and IV. The rest 30 inbred lines were contained in the admixed group. Association mapping analysis for popcorn inbred lines was performed to assess the marker-trait associations between the 50 SSR markers and the 10 morphological traits. At a significance level of 0.01, we found 92 marker-trait associations using the Q GLM model. While, using the Q+K MLM model, we found 6 marker-trait associations at a significance level of 0.01. Finally, this study's assessment of population structure and association mapping of 79 popcorn inbred lines will be helpful useful for popcorn breeding programs such activities as planning crosses for hybrid and line development at Maize Experiment Station, Gangwon Agricultural Research and Extension Services.
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